import win32com.client
import shutil

def updateSheet(Application, SourcePathName):
    Workbook = Application.Workbooks.open(SourcePathName)
    Workbook.RefreshAll()
    Workbook.Save()


Application = win32com.client.Dispatch("Excel.Application")
Application.Visible = 1

sourcePaths = ["c:/A/1/xls1", "c:/B/1/xls2", "c:/Q/45/xls3"]

for i in sourcePaths:
    updateSheet(Application, i)

Application.Quit()