def Gameplay():
    width=80
    height=80
    y = 720/2+height/2
    x = 720/2+width/2
    speed=2

    enemyspawn = False
    while True:
        #controll character here
        if enemyspawn:
            enemyspawn=False
            print(enemyspawn) #Spawn enemy here later

        window.blit(bg, [0,0])
        pygame.draw.rect(window,(100,100,100),(x,y,width,height))
        pygame.display.update()


def EnemySpawn():
    enemyspawn = EnemySpawn() #idrk about this line

    while True:
        sleep(randint(1,5))
        enemyspawn=True
        print(enemyspawn)
    return enemyspawn

Gameplay = threading.Thread(target=Gameplay)
Gameplay.start()
EnemySpawn = threading.Thread(target=EnemySpawn)
EnemySpawn.start()

line 51, in EnemySpawn
    enemyspawn = Gameplay(enemyspawn)
UnboundLocalError: local variable 'enemyspawn' referenced before assignment

line 51, in EnemySpawn
    enemyspawn = EnemySpawn()
TypeError: 'Thread' object is not callable