Ian Aranha                                                                            Junior Software Engineer

Seattle, WA 98177                                            206-420-0209_|_ianorama@gmail.com_|_linkedin.com/in/ianaranha_

Self-motivated, result driven web/software developer with a strong understanding of web development and a passion to develop well-written code. Track record of success in independent and team-driven environments, building strong team dynamics to effectively communicate and achieve shared goals.  Great communication skills, and the ability to thrive in a fast-paced environment. Looking to work with fun and creative team to transform good ideas into great products.

**Technical Skills**

OPERATING SYSTEMS: Mac OSX, Windows 7.0

LANGUAGES: JavaScript, Python, SQL, C#, HTML, CSS, Bootstrap

FRAMEWORK: AngularJS, Express.js, Socket.io, Django, Flask, ASP.NET Core, MVC, React, Redux

DATABASE: MySQL, SQLite, NoSQL, MongoDB, Building ERDs

Others: GitHub, jQuery, node.js, Bower, NPM, Microsoft Office, Mac Productivity, Google Documents, Responsive Web Design Projects

**Web Development Experience**

**Coding Dojo |** Full – Stack Web Development Student | Seattle | 2017 - 2018 **       **

Completed an immersive web development program with more than 1,000 hours spent learning and developing web applications using Python, MEAN, JavaScript,  and C# ASP.NET Core MVC framework.

**Projects** :

- Trip Planner (Python) – A trip planning application with share feature among registered users
  - Structured the database and implemented the front end design plus deploying the app using Amazon AWS (EC2) within 4.5 hours
  - Developed using Python, Django, MySQL, HTML, CSS
- Wedding Planner(C#/.Net) - An event planning application which allows user to keep track of guests attending.
  - Structured the database and implemented the front end design plus deploying the app using Amazon AWS (EC2) within 4.5 hours
  - Developed using C#, DotNet,  MySQL, HTML, CSS
- PacMan (JavaScript/CSS/HTML)
  - A basic functional pacman game developed using JavaScript.
- Functional Stopwatch and Stack using C#/.Net
- Responsive Web Design projects:
  - Tribute Page
  - Survey Form
  - Product Landing Page
  - Technical Documentation Page
  - Personal Portfolio page
- Algorithm Challenges, Data structures.
- Experience with GitHub, Cloud Computing : Amazon Web Services and Microsoft Azure.

**P**** rofessional Experience**

Sanofi – Genzyme| Lynnwood, WA | 2003 to 2017

**SENIOR DEVELOPMENT ASSOCIATE**

Spearheaded development, transfer, and characterization of downstream process capture step for purifying Leukine, a therapeutic peptide expressed by yeast. Screened various chromatography resins during process development, including Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity and Size-Exclusion modes.  Coordinated development of Tangential Flow Filtration and Depth Filtration methods.Integrated extensive knowledge of ICH guidelines to devise quality control assays.Maintained comprehensive documentation of all processes, preparing development reports and batch records to secure continuous adherence to established quality standards and regulatory requirements.

_Key Accomplishments:_

- Led the design, operation and development of Leukine capture step using scaled-down models. Oversaw transfer and scale-up of process to production for Engineering and Conformance runs. **Led collection of data** during transfer to demonstrate product quality, quantity and consistency. Used Microsoft Excel to demonstrate product yield and to calculate % Yield.   **Used statistical methods** to demonstrate equivalency of production process and scaled-down models.
- Using statistical software,  developed a Quality by Design approach to process characterization. Analyzed operating parameters to characterize process operating space and identify failure points; utilized scaled-down models, JMP statistical software, and Design of Experiments (DOE) to fully characterize the Leukine production capture step.
- Collaborated with various team members to advance purification steps, resulting in 99.8% pure GM-CSF.
- Developed quantitative assay to measure proteolytic activity in Bulk Drug Substance (BDS); created 1/200th scale model to investigate and isolate contaminating proteases to significantly improve substance shelf-life.
- Prioritized process improvement experiments, replacing existing Waters HPLC assay with Waters UPLC assay with resultant processing time reduction from one hour to 25 minutes per sample.
- Authored batch records for entire downstream purification process for PD lab. Batch records used as template starting point for official at-scale downstream production batch records.  Authored Development reports for use in CMC filings with regulatory authorities. Summarized results of experiments in PowerPoint for senior management to drive business decisions.

**ADDITIONAL EXPERIENCE       **

**DEVELOPMENT ASSOCIATE** | Targeted Genetics Corporation| Seattle, WA

- Conceptualized and developed production processes for innovative biotechnology company, acknowledged for delivering high-quality results within designated research parameters and against strict deadlines.
- Devised production processes for purification of recombinant Adeno-Associated Virus (rAAV) vectors, creating scale models for purification of rAAV forCystic Fibrosis and Rheumatoid Arthritis; approved by FDA for human clinical trials.
- Completed cell-based infectivity assay, DNA slot blot assay, SDS-PAGE and Western Blots for assessing vector purity.
- Served as lead downstream operator, orchestrating generation of purified research vector to conduct pipeline testing of viral vectors in animal models.

**EDUCATION & CREDENTIALS**

**Coding Dojo        Seattle, WA  2017 - 2018 |** Full Stack Web Development {Python, MEAN, C#, ASP.NET Core}

**Bachelor of Science in Biochemistry, Zoology Minor** | UNIVERSITY OF BOMBAY | Mumbai, India

**Associate of Science in Biotechnology** | SEATTLE CENTRAL COLLEGE | Seattle, WA

**PATENTS,  PUBLICATIONS & PRESENTATIONS**

**US**** Patent ****US**** 6,995,006 ****B2**** : ****Methods**** For ****Generating**** High ****Titer**** Helper-free ****Preparations**** Of ****Released**** Recombinant ****AAV**** Vectors. **Edward M. Atkinson, Victor P. Fung, Perry C. Wilkins, Ryan K. Takeya, Thomas C. Reynolds** , ****Ian L. Aranha**** ;** Assignee: Targeted Genetics Corporation, Seattle, WA.

**Process**** Development ****for**** Manufacturing ****of**** Gene ****Therapy**** Vectors: ****Recovery**** of ****Adenovirus**** and ****Recombinant**** Adeno-Associated ****Virus**** Vectors. **E. Morrey Atkinson,** Ian Aranha**, Daryn Debelak, Thomas C. Reynolds and Victor P. Fung, Targeted Genetics Corp., Seattle; WA; USA.

Presented at Recovery of Biological Products - VIII; Tucson; AZ, 20-25 October 1996.

**Development**** of ****a**** Protease ****Assay**** for ****Leukine**** and ****the**** Use ****of**** the ****Assay**** to ****Characterize**** the ****Reduction**** of ****Protease**** in ****the**** Purification ****Process**. **I. Aranha,** J.Yost, J. Robinson.

Presented at 2013 Manufacturing Innovation Summit - Genzyme, Framingham; MA; USA.