test.py

from tkinter import *

def azzera():
    for widget in test.winfo_children():
        widget.place_forget()

def start():
    azzera()
    #qua metti la parte per tuo test

test=Tk()
test.title("TEMPS-A TEST")
test.geometry("500x500")
test.resizable(0,0)

L1=Label(text="BENVENUTO\n",font= ("Courier Now", 11, "bold"))
L1.place(relx=0.5,y=180,anchor='center')
L2=Label(text="----------\nClicca qui",font= ("Courier Now", 11))
L2.place(relx=0.5,y=200,anchor='center')
B1=Button(test,text="START",font=("Courier Now", 11),command=start)
B1.place(relx=0.5,y=250,anchor='center')

test.mainloop()