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bcl2fastq error

Dec 8th, 2021
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  1. /mnt/scgc_nfs/opt/common/anaconda/2.1.0/lib/python2.7/site-packages/matplotlib/__init__.py:872: UserWarning: axes.color_cycle is deprecated and replaced with axes.prop_cycle; please use the latter.
  2. warnings.warn(self.msg_depr % (key, alt_key))
  3. [2021-12-08 09:55:19 - INFO] Using /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX/SampleSheet.csv
  4. [2021-12-08 09:55:19 - INFO] Run complete.
  5. [2021-12-08 13:55:19 - INFO] Converting a subset to determine barcodes using: $>bcl2fastq --tiles 1105 --no-lane-splitting --runfolder-dir /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX --output-dir /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX/tmpcOExZh --barcode-mismatches 0 --loading-threads 12 --processing-threads 40 --writing-threads 12 --sample-sheet /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX/SampleSheet.csv.2021-12-08-1355-19.rc.csv
  6. Traceback (most recent call last):
  7. File "/mnt/scgc_nfs/opt/common/anaconda/2.1.0/bin/bcl_to_fastq", line 11, in <module>
  8. load_entry_point('bcl2fastq==1.3.0', 'console_scripts', 'bcl_to_fastq')()
  9. File "/mnt/scgc_nfs/opt/common/anaconda/2.1.0/lib/python2.7/site-packages/click/core.py", line 722, in __call__
  10. return self.main(*args, **kwargs)
  11. File "/mnt/scgc_nfs/opt/common/anaconda/2.1.0/lib/python2.7/site-packages/click/core.py", line 697, in main
  12. rv = self.invoke(ctx)
  13. File "/mnt/scgc_nfs/opt/common/anaconda/2.1.0/lib/python2.7/site-packages/click/core.py", line 895, in invoke
  14. return ctx.invoke(self.callback, **ctx.params)
  15. File "/mnt/scgc_nfs/opt/common/anaconda/2.1.0/lib/python2.7/site-packages/click/core.py", line 535, in invoke
  16. return callback(*args, **kwargs)
  17. File "build/bdist.linux-x86_64/egg/bcl2fastq.py", line 558, in bcl2fastq
  18. File "build/bdist.linux-x86_64/egg/bcl2fastq.py", line 209, in run_determination_step
  19. File "build/bdist.linux-x86_64/egg/bcl2fastq.py", line 162, in run_bcl2fastq
  20. File "/mnt/scgc_nfs/opt/common/anaconda/2.1.0/lib/python2.7/subprocess.py", line 190, in check_call
  21. raise CalledProcessError(retcode, cmd)
  22. subprocess.CalledProcessError: Command 'bcl2fastq --tiles 1105 --no-lane-splitting --runfolder-dir /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX --output-dir /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX/tmpcOExZh --barcode-mismatches 0 --loading-threads 12 --processing-threads 40 --writing-threads 12 --sample-sheet /mnt/scgc_raw/data/nextseq/180228_NB502014_0078_AHVL5JAFXX/SampleSheet.csv.2021-12-08-1355-19.rc.csv' returned non-zero exit status 1
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