AutoAvailable 1.2 (GUI)

import time
import threading
from tkinter import *
import pyautogui
import random
import keyboard

root = Tk()
root.title("Loop Terminate")

time.sleep(0.5)

stop = False

def button_stop_command():
  # If the STOP button is pressed then terminate the loop
  global stop
  stop = True

def button_start_command():
  global stop
  stop = False
  time.sleep(2)
  pyautogui.hotkey('win', 'd')
  while True and not stop:
    MouseX = random.randint(0, 1300)
    MouseY = random.randint(0, 700)
    MouseSleep = random.randint(1, 5)
    pyautogui.moveTo(MouseX, MouseY, duration=1)
    pyautogui.click()
    time.sleep(MouseSleep)

def button_starter():
  t = threading.Thread(target=button_start_command)
  t.start()

# Button START
button_start = Button(root, text="START", padx=30, pady=20, command=button_starter)
button_start.grid(columnspan=1, row=1,column=0)

# Button STOP
button_stop = Button(root, text="STOP", padx=33, pady=20, command=button_stop_command)
button_stop.grid(row=2, column=0)

root.mainloop()