Primer3 Output

WARNING: Numbers in input sequence were deleted.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER       1549   20   59.13   50.00  3.00  1.00 10.00 tcaggttacggacaaggtga
RIGHT PRIMER      1851   20   59.84   45.00  5.00  3.00 11.00 aaaccacccaatttgtctgc
SEQUENCE SIZE: 2129
INCLUDED REGION SIZE: 2129

PRODUCT SIZE: 303, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

    1 atgaaaaagataaaaattgttccacttattttaatagttgtagttgtcgggtttggtata


   61 tatttttatgcttcaaaagataaagaaattaataatactattgatgcaattgaagataaa


  121 aatttcaaacaagtttataaagatagcagttatatttctaaaagcgataatggtgaagta


  181 gaaatgactgaacgtccgataaaaatatataatagtttaggcgttaaagatataaacatt


  241 caggatcgtaaaataaaaaaagtatctaaaaataaaaaacgagtagatgctcaatataaa


  301 attaaaacaaactacggtaacattgatcgcaacgttcaatttaattttgttaaagaagat


  361 ggtatgtggaagttagattgggatcatagcgtcattattccaggaatgcagaaagaccaa


  421 agcatacatattgaaaatttaaaatcagaacgtggtaaaattttagaccgaaacaatgtg


  481 gaattggccaatacaggaacagcatatgagataggcatcgttccaaagaatgtatctaaa


  541 aaagattataaagcaatcgctaaagaactaagtatttctgaagactatatcaaacaacaa


  601 atggatcaaaattgggtacaagatgataccttcgttccacttaaaaccgttaaaaaaatg


  661 gatgaatatttaagtgatttcgcaaaaaaatttcatcttacaactaatgaaacaaaaagt


  721 cgtaactatcctctagaaaaagcgacttcacatctattaggttatgttggtcccattaac


  781 tctgaagaattaaaacaaaaagaatataaaggctataaagatgatgcagttattggtaaa


  841 aagggactcgaaaaactttacgataaaaagctccaacatgaagatggctatcgtgtcaca


  901 atcgttgacgataatagcaatacaatcgcacatacattaatagagaaaaagaaaaaagat


  961 ggcaaagatattcaactaactattgatgctaaagttcaaaagagtatttataacaacatg


 1021 aaaaatgattatggctcaggtactgctatccaccctcaaacaggtgaattattagcactt


 1081 gtaagcacaccttcatatgacgtctatccatttatgtatggcatgagtaacgaagaatat


 1141 aataaattaaccgaagataaaaaagaacctctgctcaacaagttccagattacaacttca


 1201 ccaggttcaactcaaaaaatattaacagcaatgattgggttaaataacaaaacattagac


 1261 gataaaacaagttataaaatcgatggtaaaggttggcaaaaagataaatcttggggtggt


 1321 tacaacgttacaagatatgaagtggtaaatggtaatatcgacttaaaacaagcaatagaa


 1381 tcatcagataacattttctttgctagagtagcactcgaattaggcagtaagaaatttgaa


 1441 aaaggcatgaaaaaactaggtgttggtgaagatataccaagtgattatccattttataat


 1501 gctcaaatttcaaacaaaaatttagataatgaaatattattagctgattcaggttacgga
                                                      >>>>>>>>>>>>

 1561 caaggtgaaatactgattaacccagtacagatcctttcaatctatagcgcattagaaaat
      >>>>>>>>

 1621 aatggcaatattaacgcacctcacttattaaaagacacgaaaaacaaagtttggaagaaa


 1681 aatattatttccaaagaaaatatcaatctattaactgatggtatgcaacaagtcgtaaat


 1741 aaaacacataaagaagatatttatagatcttatgcaaacttaattggcaaatccggtact


 1801 gcagaactcaaaatgaaacaaggagaaactggcagacaaattgggtggtttatatcatat
                                     <<<<<<<<<<<<<<<<<<<<

 1861 gataaagataatccaaacatgatgatggctattaatgttaaagatgtacaagataaagga


 1921 atggctagctacaatgccaaaatctcaggtaaagtgtatgatgagctatatgagaacggt


 1981 aataaaaaatacgatatagatgaataacaaaacagtgaagcaatccgtaacgatggttgc


 2041 ttcactgttttattatgaattattaataagtgctgttacttctcccttaaatacaatttc


 2101 ttcattttcattgtatgttgaaagtgaca


KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3'   rep seq

 1 LEFT PRIMER       1545   21   59.98   47.62  5.00  0.00 10.00 tgattcaggttacggacaagg
   RIGHT PRIMER      1845   20   59.97   45.00  5.00  3.00 11.00 cccaatttgtctgccagttt
   PRODUCT SIZE: 301, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1543   21   60.13   47.62  7.00  0.00  9.00 gctgattcaggttacggacaa
   RIGHT PRIMER      1845   20   59.97   45.00  5.00  3.00 11.00 cccaatttgtctgccagttt
   PRODUCT SIZE: 303, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       1550   21   60.02   47.62  3.00  0.00 11.00 caggttacggacaaggtgaaa
   RIGHT PRIMER      1851   20   59.84   45.00  5.00  3.00 11.00 aaaccacccaatttgtctgc
   PRODUCT SIZE: 302, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1545   21   59.98   47.62  5.00  0.00 10.00 tgattcaggttacggacaagg
   RIGHT PRIMER      1851   20   59.84   45.00  5.00  3.00 11.00 aaaccacccaatttgtctgc
   PRODUCT SIZE: 307, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

Statistics
         con   too    in    in          no    tm    tm  high  high  high        high
         sid  many   tar  excl   bad    GC   too   too   any    3'   lib  poly   end
        ered    Ns   get   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left   16643     0     0     0  2226     0  9335  1128     0     1     0   172   111  3670
Right  16706     0     0     0  1748     0  9429  1278     0     3     0   166   109  3973
Pair Stats:
considered 1312, unacceptable product size 1303, high end compl 1, ok 8
primer3 release 1.1.4


(primer3_results.cgi release 0.4.0)