IAPV/dsRNA in Honeybees Experiment

IAPV Experiment:
Part 1
1) Inject five honeybees with the IAPV virus (write down the time and day you do this) leaving another five alone (control).
2) Watch the bees for one week, making note of all symptoms and behaviors of the virus in the bees
3) Make note of the bees' mortality rate with IAPV compared to the date you injected the virus
4) Compare the experimental bees with the control bees (mortality rate), write down observations and conclusions

Part 2
1) Inject five honeybees with IAPV, leaving the five control bees alone
2) Feed dsRNA to the five experimental bees on a constant basis every day for the week
3) Make note of the bees' mortality rate compared to the date you injected the virus
4) Compare the experimental bees' mortality rate and the control bees' mortality rate, and write down observations and conclusions about the effects that the dsRNA had on the IAPV.



Notes:
IAPV infection results in more significant changes in gene expression in adult bees than in brood
High genetic diversity exists between different strains of IAPV