var testef = [{
"Id": 1,
"Latitude": 38.726177,
"Longitude": -9.180716
},
{
"Id": 2,
"Latitude": 38.716177,
"Longitude": -9.170716
},
{
"Id": 3,
"Latitude": 38.736177,
"Longitude": -9.160716
},
 {
"Id": 4,
"Latitude": 38.729177,
"Longitude": -9.110716
 }];

 //traçando a rota
for(var k=0; k < testef.length; k++){
    var objk=testef[k];

    var mypath = new google.maps.LatLng(objk.Latitude,objk.Longitude);

    var teste = new google.maps.Polyline({
        path: mypath,
        geodesic: true,
        strokeColor: '#ff0000',
        strokeOpacity: 1.0,
        strokeWeight: 3
    });
}

  teste.setMap(map);