Aim: To Detemine if organisms will spontainoulsy generate from non living organic molecules in broth
Hypothis: The broth with the straight tube will develope organisims however the bent flasked broth will not
Theory: In the 1800's pasteur tested the validity of a widly accpeted theory of spontanious generation of organisms without parental organisms. In doing so he hoped to determine the cause of microbacterial growth on foods (ie cheese)
Equitment:
2x conical flask  400-500mL
2x Rubber stoppers with holes to suit glass tubes and 400-500mL flasks
2x Glass tubes 20cm length x 1cm or < diameter
200mL chiken broth
1x Bunsen burner
1x tripod
1x gauze matt
1x tongs
Method:
Collect equitment
Place 100mL of broth in each flask
Set up bunsen burner
Heat up and melt 1 glass tube into an S shape allowing enoguh space for the tube in the flask
Place glass tubes into rubber stoppers
Pour 100mL of broth into each flask using mesuring cylinder
Place stoppers with tubes inside of the flasks
set up tripod and gauze mat
heat flasks untill broth starts boiling
boil broth for 5 minutes to steralise it
Allow flasks to cool and place in a location where they will not be disturbed for 2 weeks
Record observations every 2 days
Repeat experiment to confirm observations and results
Results:

Day1:
Day3:
Day5:
Day7:
Day9:
Day11:
Day13:
Day 14:
Discussion:
Conclusion: