Errore Script Python

[root@localhost denovo_28June]# python
Python 2.7.15 |Anaconda, Inc.| (default, Dec 14 2018, 19:04:19)
[GCC 7.3.0] on linux2
Type "help", "copyright", "credits" or "license" for more information.
>>> import re
>>> with open ('FD.fastq', 'r' ) as f:
...     content = f.read()
... content_new = re.sub('(@[0-9A-Z]]*) [^\\r\\n]*', r'\1/1', content, flags = re.M)
  File "<stdin>", line 3
    content_new = re.sub('(@[0-9A-Z]]*) [^\\r\\n]*', r'\1/1', content, flags = re.M)
              ^
SyntaxError: invalid syntax
>>> f = open('output.txt', 'w')
>>> f.write(content_new)
Traceback (most recent call last):
  File "<stdin>", line 1, in <module>
NameError: name 'content_new' is not defined
>>> f.close()