img = cv2.imread(path, -1)
eye = img[349:307, 410:383]
img[30:180, 91:256] = eye

Traceback (most recent call last):
  File "test.py", line 13, in <module>
    img[30:180, 91:256] = eye
ValueError: could not broadcast input array from shape (0,0,3) into shape (150,165,3)

ValueError: could not broadcast input array from shape (0,0,3) into shape (150,165,3)

In [8]: import cv2
   ...: fn=r'D:DocumentsDesktop1.jpg'
   ...: img=cv2.imread(fn, -1)
   ...: roi=img[200:400, 200:300]

In [9]: roi.shape
Out[9]: (200, 100, 3)

In [10]: img2=img.copy()

In [11]: img2[:roi.shape[0], :roi.shape[1]]=roi

In [12]: cv2.imshow('img', img)
    ...: cv2.imshow('roi', roi)
    ...: cv2.imshow('img2', img2)
    ...: cv2.waitKey(0)
    ...: cv2.destroyAllWindows()

In [13]: img2[:100, :100]=roi
---------------------------------------------------------------------------
ValueError                                Traceback (most recent call last)
<ipython-input-13-85de95cf3ded> in <module>()
----> 1 img2[:100, :100]=roi

ValueError: could not broadcast input array from shape (200,100,3) into shape (100,100,3)

img[349:307, 410:383]

img[307,349, 383:413]