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  1. *** sample.sam 2018-12-14 14:38:05.192150444 +0100
  2. --- D8_Bird8_42-3p.sam 2018-12-14 09:54:48.044613052 +0100
  3. ***************
  4. *** 166580,166588 ****
  5. FCC4MT3ACXX:7:2313:6760:33545# 147 PRELSG_09_v1 153282 255 75M = 153188 -169 AAAAAAATGGAAGAGGAATATATATTGATAGGAAATTAACAAAATATAAATGTACTTGGAAAGACGATAAAGTTT BDFEFDCEACEHA:GEED;@GGIIIIHGGBGEGGGGFDDGIGGGGHHGCIIGB?JIIGDD:8:?GFAEHBFHEAH NH:i:1 HI:i:1 AS:i:144 nM:i:0 MD:Z:75
  6. FCC4MT3ACXX:7:2105:17732:30026# 163 PRELSG_09_v1 153780 255 5S70M = 153836 130 ATGGGTAAAAAAGAAGGAAATTAAAGATTTATTTTGTTCAAGTTCTTACGATTCTTCATTATCAGATTCAAGTCT CHHDH:?BGIBGIG@BGBG@BGIEFGHIIGHIHIIGCHHHHF=EHFFFFBADCCEDCDCCCCDCDDDDDDEDACC NH:i:1 HI:i:1 AS:i:132 nM:i:3 MD:Z:70
  7. FCC4MT3ACXX:7:2105:17732:30026# 83 PRELSG_09_v1 153836 255 26M1I48M = 153780 -130 TCAGATTCAAGGCTCAATAAAATCTTAAAAAAAAAAAAAAAATAATAATAAAAATAAAAAAAAGAGACAATACAT ##########################BBDBDDDDDDHJJIGIGHGGIHAIIIIHCIJIJJGIGIIJHGGGDHIIG NH:i:1 HI:i:1 AS:i:132 nM:i:3 MD:Z:11T4T3T53
  8. ! FCC4MT3ACXX:7:2301:5901:17976# 147 PRELSG_09_v1 153992 1 25M137386N50M = 94300 -197153 AAGGGAAAAAAAAAAAAAAAAAAAAATTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA #################################DDB@@;7505BDDDDDDDDDDDDDDDDDDDHIJJJJJJJJII NH:i:3 HI:i:1 AS:i:131 nM:i:0 MD:Z:2500000043
  9. FCC4MT3ACXX:7:2301:5901:17976# 403 PRELSG_09_v1 153992 1 34M70796N41M = 94300 -130563 AAGGGAAAAAAAAAAAAAAAAAAAAATTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA #################################DDB@@;7505BDDDDDDDDDDDDDDDDDDDHIJJJJJJJJII NH:i:3 HI:i:2 AS:i:130 nM:i:8 MD:Z:25C0A0A0C0A0G0A0T42
  10. ! FCC4MT3ACXX:7:2301:5901:17976# 403 PRELSG_09_v1 153992 1 25M137385N50M = 94300 -197152 AAGGGAAAAAAAAAAAAAAAAAAAAATTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA #################################DDB@@;7505BDDDDDDDDDDDDDDDDDDDHIJJJJJJJJII NH:i:3 HI:i:3 AS:i:130 nM:i:0 MD:Z:25000000042
  11. FCC4MT3ACXX:7:2111:2622:77765# 99 PRELSG_09_v1 154313 0 10S65M = 224822 70567 GAAGCTTCAATTAGAATAAAACAAATACTTGCTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GHEIEIIIJIHJFIIHIJIIJJFFHIIJEHIJJIJIGHJJJGGFDDBBBBBBDDDBDDDDDB59<<>BDBDDDDB NH:i:8 HI:i:1 AS:i:101 nM:i:9 MD:Z:6G2T2G2A1T0C0A0T0A43
  12. FCC4MT3ACXX:7:2111:2622:77765# 355 PRELSG_09_v1 154313 0 10S65M = 224823 70568 GAAGCTTCAATTAGAATAAAACAAATACTTGCTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GHEIEIIIJIHJFIIHIJIIJJFFHIIJEHIJJIJIGHJJJGGFDDBBBBBBDDDBDDDDDB59<<>BDBDDDDB NH:i:8 HI:i:2 AS:i:101 nM:i:9 MD:Z:6G2T2G2A1T0C0A0T0A43
  13. FCC4MT3ACXX:7:2111:2622:77765# 355 PRELSG_09_v1 154313 0 10S65M = 224824 70569 GAAGCTTCAATTAGAATAAAACAAATACTTGCTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GHEIEIIIJIHJFIIHIJIIJJFFHIIJEHIJJIJIGHJJJGGFDDBBBBBBDDDBDDDDDB59<<>BDBDDDDB NH:i:8 HI:i:3 AS:i:101 nM:i:9 MD:Z:6G2T2G2A1T0C0A0T0A43
  14. --- 166580,166588 ----
  15. FCC4MT3ACXX:7:2313:6760:33545# 147 PRELSG_09_v1 153282 255 75M = 153188 -169 AAAAAAATGGAAGAGGAATATATATTGATAGGAAATTAACAAAATATAAATGTACTTGGAAAGACGATAAAGTTT BDFEFDCEACEHA:GEED;@GGIIIIHGGBGEGGGGFDDGIGGGGHHGCIIGB?JIIGDD:8:?GFAEHBFHEAH NH:i:1 HI:i:1 AS:i:144 nM:i:0 MD:Z:75
  16. FCC4MT3ACXX:7:2105:17732:30026# 163 PRELSG_09_v1 153780 255 5S70M = 153836 130 ATGGGTAAAAAAGAAGGAAATTAAAGATTTATTTTGTTCAAGTTCTTACGATTCTTCATTATCAGATTCAAGTCT CHHDH:?BGIBGIG@BGBG@BGIEFGHIIGHIHIIGCHHHHF=EHFFFFBADCCEDCDCCCCDCDDDDDDEDACC NH:i:1 HI:i:1 AS:i:132 nM:i:3 MD:Z:70
  17. FCC4MT3ACXX:7:2105:17732:30026# 83 PRELSG_09_v1 153836 255 26M1I48M = 153780 -130 TCAGATTCAAGGCTCAATAAAATCTTAAAAAAAAAAAAAAAATAATAATAAAAATAAAAAAAAGAGACAATACAT ##########################BBDBDDDDDDHJJIGIGHGGIHAIIIIHCIJIJJGIGIIJHGGGDHIIG NH:i:1 HI:i:1 AS:i:132 nM:i:3 MD:Z:11T4T3T53
  18. ! FCC4MT3ACXX:7:2301:5901:17976# 147 PRELSG_09_v1 153992 1 25M137386N50M = 94300 -197153 AAGGGAAAAAAAAAAAAAAAAAAAAATTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA #################################DDB@@;7505BDDDDDDDDDDDDDDDDDDDHIJJJJJJJJII NH:i:3 HI:i:1 AS:i:131 nM:i:0 MD:Z:25�0�0�0�0�0�0�43
  19. FCC4MT3ACXX:7:2301:5901:17976# 403 PRELSG_09_v1 153992 1 34M70796N41M = 94300 -130563 AAGGGAAAAAAAAAAAAAAAAAAAAATTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA #################################DDB@@;7505BDDDDDDDDDDDDDDDDDDDHIJJJJJJJJII NH:i:3 HI:i:2 AS:i:130 nM:i:8 MD:Z:25C0A0A0C0A0G0A0T42
  20. ! FCC4MT3ACXX:7:2301:5901:17976# 403 PRELSG_09_v1 153992 1 25M137385N50M = 94300 -197152 AAGGGAAAAAAAAAAAAAAAAAAAAATTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA #################################DDB@@;7505BDDDDDDDDDDDDDDDDDDDHIJJJJJJJJII NH:i:3 HI:i:3 AS:i:130 nM:i:0 MD:Z:25�0�0�0�0�0�0�0�42
  21. FCC4MT3ACXX:7:2111:2622:77765# 99 PRELSG_09_v1 154313 0 10S65M = 224822 70567 GAAGCTTCAATTAGAATAAAACAAATACTTGCTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GHEIEIIIJIHJFIIHIJIIJJFFHIIJEHIJJIJIGHJJJGGFDDBBBBBBDDDBDDDDDB59<<>BDBDDDDB NH:i:8 HI:i:1 AS:i:101 nM:i:9 MD:Z:6G2T2G2A1T0C0A0T0A43
  22. FCC4MT3ACXX:7:2111:2622:77765# 355 PRELSG_09_v1 154313 0 10S65M = 224823 70568 GAAGCTTCAATTAGAATAAAACAAATACTTGCTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GHEIEIIIJIHJFIIHIJIIJJFFHIIJEHIJJIJIGHJJJGGFDDBBBBBBDDDBDDDDDB59<<>BDBDDDDB NH:i:8 HI:i:2 AS:i:101 nM:i:9 MD:Z:6G2T2G2A1T0C0A0T0A43
  23. FCC4MT3ACXX:7:2111:2622:77765# 355 PRELSG_09_v1 154313 0 10S65M = 224824 70569 GAAGCTTCAATTAGAATAAAACAAATACTTGCTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GHEIEIIIJIHJFIIHIJIIJJFFHIIJEHIJJIJIGHJJJGGFDDBBBBBBDDDBDDDDDB59<<>BDBDDDDB NH:i:8 HI:i:3 AS:i:101 nM:i:9 MD:Z:6G2T2G2A1T0C0A0T0A43
  24. ***************
  25. *** 269512,269518 ****
  26. FCC4MT3ACXX:7:1203:4704:43923# 403 PRELSG_12_v1 1145305 0 70M5S = 1145148 -227 ATTTGAACTATTTGACTCACTTGAATCATTTGAATCATATGAATCACCTGAATATGATGAAATAGATATTCCCAT ####@>@EEDC:D@7)=;=7CA=.)>EHGHEGA=G>CIHAHCBD?/FB<99**GIGGDB:?DHFC<D?DD3@FAG NH:i:6 HI:i:4 AS:i:131 nM:i:6 MD:Z:7T0C1C4A3T18T31
  27. FCC4MT3ACXX:7:1203:4704:43923# 403 PRELSG_12_v1 1145305 0 70M5S = 1145121 -254 ATTTGAACTATTTGACTCACTTGAATCATTTGAATCATATGAATCACCTGAATATGATGAAATAGATATTCCCAT ####@>@EEDC:D@7)=;=7CA=.)>EHGHEGA=G>CIHAHCBD?/FB<99**GIGGDB:?DHFC<D?DD3@FAG NH:i:6 HI:i:5 AS:i:131 nM:i:6 MD:Z:7T0C1C4A3T18T31
  28. FCC4MT3ACXX:7:1203:4704:43923# 403 PRELSG_12_v1 1145305 0 70M5S = 1145094 -281 ATTTGAACTATTTGACTCACTTGAATCATTTGAATCATATGAATCACCTGAATATGATGAAATAGATATTCCCAT ####@>@EEDC:D@7)=;=7CA=.)>EHGHEGA=G>CIHAHCBD?/FB<99**GIGGDB:?DHFC<D?DD3@FAG NH:i:6 HI:i:6 AS:i:131 nM:i:6 MD:Z:7T0C1C4A3T18T31
  29. ! FCC4MT3ACXX:7:2306:14353:11414# 99 PRELSG_12_v1 1145351 255 67M8S = 1145416 135 CCTGAATATGATGAAATAGATATTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT IIIIGCHIIIHIIGDGHIH4C?F>DFGIDBAFIIIIIHFDDDDDDDDDDD<@<<<&09>>BDB@BBB@####### NH:i:1 HI:i:1 AS:i:128 nM:i:3 MD:Z:2923C0C12
  30. FCC4MT3ACXX:7:1104:18310:22638# 147 PRELSG_12_v1 1145381 255 37M38S = 1145298 -120 TTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTCTTATCTTGTGATGAAATACTACTTTCACTATCCCAT B<9<3BB@9>@BB<<DDBBCA>2(&@B?:DCDFIJJIIHCGGCGJHFBD??BH@BF<DBBGH?B<GD?C9=F?:? NH:i:1 HI:i:1 AS:i:100 nM:i:5 MD:Z:37
  31. FCC4MT3ACXX:7:2201:14317:58284# 163 PRELSG_12_v1 1145397 255 75M = 1145462 140 TTTTTTTCCTTTTTTTTTTTTCTCCATTATGTTCTACTTTATTCGAATGATTCTCTTTCTTGTCATAACTTAAAT JIJJJJJIJJJJJJJGIHDDD9:>CCAB@C>CCAC:@@A@34>:<A?@CCCD3::@CA@:@@>:(:@(:AC:@## NH:i:1 HI:i:1 AS:i:148 nM:i:0 MD:Z:75
  32. FCC4MT3ACXX:7:2306:14353:11414# 147 PRELSG_12_v1 1145416 255 70M5S = 1145351 -135 TTCTCCATTATGTTCTGCTTTATTCGAATGATTCTCTTTCTTGTCATAACTTAAATCTTCTATTTTATCTCCCAT ?AA>:CC>3CCC?A@>3EECFEFEFHHHFHEGIIIJJJIIJJJIJIHD?9FCIJIGGCEJIIGJJIEEIHIHHIG NH:i:1 HI:i:1 AS:i:128 nM:i:3 MD:Z:16A53
  33. --- 269512,269518 ----
  34. FCC4MT3ACXX:7:1203:4704:43923# 403 PRELSG_12_v1 1145305 0 70M5S = 1145148 -227 ATTTGAACTATTTGACTCACTTGAATCATTTGAATCATATGAATCACCTGAATATGATGAAATAGATATTCCCAT ####@>@EEDC:D@7)=;=7CA=.)>EHGHEGA=G>CIHAHCBD?/FB<99**GIGGDB:?DHFC<D?DD3@FAG NH:i:6 HI:i:4 AS:i:131 nM:i:6 MD:Z:7T0C1C4A3T18T31
  35. FCC4MT3ACXX:7:1203:4704:43923# 403 PRELSG_12_v1 1145305 0 70M5S = 1145121 -254 ATTTGAACTATTTGACTCACTTGAATCATTTGAATCATATGAATCACCTGAATATGATGAAATAGATATTCCCAT ####@>@EEDC:D@7)=;=7CA=.)>EHGHEGA=G>CIHAHCBD?/FB<99**GIGGDB:?DHFC<D?DD3@FAG NH:i:6 HI:i:5 AS:i:131 nM:i:6 MD:Z:7T0C1C4A3T18T31
  36. FCC4MT3ACXX:7:1203:4704:43923# 403 PRELSG_12_v1 1145305 0 70M5S = 1145094 -281 ATTTGAACTATTTGACTCACTTGAATCATTTGAATCATATGAATCACCTGAATATGATGAAATAGATATTCCCAT ####@>@EEDC:D@7)=;=7CA=.)>EHGHEGA=G>CIHAHCBD?/FB<99**GIGGDB:?DHFC<D?DD3@FAG NH:i:6 HI:i:6 AS:i:131 nM:i:6 MD:Z:7T0C1C4A3T18T31
  37. ! FCC4MT3ACXX:7:2306:14353:11414# 99 PRELSG_12_v1 1145351 255 67M8S = 1145416 135 CCTGAATATGATGAAATAGATATTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT IIIIGCHIIIHIIGDGHIH4C?F>DFGIDBAFIIIIIHFDDDDDDDDDDD<@<<<&09>>BDB@BBB@####### NH:i:1 HI:i:1 AS:i:128 nM:i:3 MD:Z:29�23C0C12
  38. FCC4MT3ACXX:7:1104:18310:22638# 147 PRELSG_12_v1 1145381 255 37M38S = 1145298 -120 TTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTCTTATCTTGTGATGAAATACTACTTTCACTATCCCAT B<9<3BB@9>@BB<<DDBBCA>2(&@B?:DCDFIJJIIHCGGCGJHFBD??BH@BF<DBBGH?B<GD?C9=F?:? NH:i:1 HI:i:1 AS:i:100 nM:i:5 MD:Z:37
  39. FCC4MT3ACXX:7:2201:14317:58284# 163 PRELSG_12_v1 1145397 255 75M = 1145462 140 TTTTTTTCCTTTTTTTTTTTTCTCCATTATGTTCTACTTTATTCGAATGATTCTCTTTCTTGTCATAACTTAAAT JIJJJJJIJJJJJJJGIHDDD9:>CCAB@C>CCAC:@@A@34>:<A?@CCCD3::@CA@:@@>:(:@(:AC:@## NH:i:1 HI:i:1 AS:i:148 nM:i:0 MD:Z:75
  40. FCC4MT3ACXX:7:2306:14353:11414# 147 PRELSG_12_v1 1145416 255 70M5S = 1145351 -135 TTCTCCATTATGTTCTGCTTTATTCGAATGATTCTCTTTCTTGTCATAACTTAAATCTTCTATTTTATCTCCCAT ?AA>:CC>3CCC?A@>3EECFEFEFHHHFHEGIIIJJJIIJJJIJIHD?9FCIJIGGCEJIIGJJIEEIHIHHIG NH:i:1 HI:i:1 AS:i:128 nM:i:3 MD:Z:16A53
  41. ***************
  42. *** 307047,307056 ****
  43. FCC4MT3ACXX:7:2105:5964:7144# 339 PRELSG_13_v1 1170699 3 70M5S = 1170532 -237 TTCATCGGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCGTCGCCCAT CCBDDDDDDDDCADDDDDDDEFDCCDDDDDDCDDDEEEDEFFFFFFFGHHHHJJJJJJIIHEJIJJJJJJJJJIJ NH:i:2 HI:i:2 AS:i:141 nM:i:1 MD:Z:66A3
  44. FCC4MT3ACXX:7:2111:4162:2811# 99 PRELSG_13_v1 1170699 3 5S70M = 1170887 263 TTATCTTCATCTGTTTCACTTTCTTCAATATCTTCTTTTCCATCTTCAATAATTTCTTCATTATCTTTTTCATCG JJIGIIIJIIJJIHHJIEGGHIIJJIG@EDHIJIGIIIIJJIJJJIGGHGIIIIHGIGIIBHEHFHFHHFFDDDD NH:i:2 HI:i:1 AS:i:129 nM:i:7 MD:Z:6G4T22T10C24
  45. FCC4MT3ACXX:7:2313:16909:82298# 403 PRELSG_13_v1 1170699 3 70M5S = 1170587 -182 TTCATCGGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCACCGCCCAT ::<<9B<@:CA:+4(DDDDDCCA@CA?9?BA>@@DC>;>>;BA@CBC@HCA7?774GIIIIGCB-@FHDBGCIIH NH:i:2 HI:i:2 AS:i:141 nM:i:1 MD:Z:67T2
  46. ! FCC4MT3ACXX:7:2115:15127:71640# 163 PRELSG_13_v1 1170704 3 74M1S = 1170928 294 CGGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTGTTTCACCGGTTTCATTTT JIJD@HEHGHIJI<BFFIJJI@FIEHEFGGHIJJIJJIDHHIJIJJJIIIIIIJII4CEEEHHHEF=ACCEEEFE NH:i:2 HI:i:1 AS:i:135 nM:i:3 MD:Z:56T5T60T3
  47. ! FCC4MT3ACXX:7:1202:14259:28598# 403 PRELSG_13_v1 1170705 1 70M5S = 1170680 -95 GGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCATCGTTATTTCCCAT BDCDCA>ACCDDDDEEEDDEDEDFFFFDEFGGHAFIGGGEGIIIIIIIHIJJJIJJJIIICJIGFDGEGFGDHGG NH:i:3 HI:i:2 AS:i:138 nM:i:2 MD:Z:64G1T11
  48. ! FCC4MT3ACXX:7:1202:14259:28598# 403 PRELSG_13_v1 1170705 1 70M5S = 1170617 -158 GGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCATCGTTATTTCCCAT BDCDCA>ACCDDDDEEEDDEDEDFFFFDEFGGHAFIGGGEGIIIIIIIHIJJJIJJJIIICJIGFDGEGFGDHGG NH:i:3 HI:i:3 AS:i:138 nM:i:2 MD:Z:64G1T11
  49. ! FCC4MT3ACXX:7:2115:17199:58163# 339 PRELSG_13_v1 1170709 1 69M6S = 1170646 -132 TTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCACCGGTTTCATTTTCCCAT C>4>(CCC@>AEDCCCCA?@BCCAACECFFFFFFHHGHHHIIIGIGIHEGGHGGEHGEGDD?4@FC9::)?;@HA NH:i:3 HI:i:3 AS:i:137 nM:i:2 MD:Z:57T60T3
  50. FCC4MT3ACXX:7:2209:4641:95092# 99 PRELSG_13_v1 1170714 1 9M158N66M = 1170950 311 TTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCACCGGTTTCATTTTCTTTCTTTTC IJJJIIIEIBGGJJJJIJJIJJGIJJJGGHIHIIIJIJJIHHJJJIJJJIGIGGIE=BEFDEDEEEEEEEDDDDD NH:i:3 HI:i:1 AS:i:136 nM:i:2 MD:Z:75
  51. FCC4MT3ACXX:7:2113:15881:6256# 339 PRELSG_13_v1 1170780 3 70M5S = 1170305 -545 AGTCTTTTTTCATTTTCATTGTTTTTTAATTTGTCTAATTCATTAAGTAAATATCCATCCATTAATTCTTCCCAT CCB@B?CCCCCCC>C@CC=9<>?=7>CEE@;BHEGGEGIHGGHGGHGFDGCIDHIGHEFBFGGGDF>GD;CCHGG NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:70
  52. FCC4MT3ACXX:7:2113:15894:6267# 339 PRELSG_13_v1 1170782 3 2S68M5S = 1170305 -545 ATTCTTTTTTCATTTTCATTGTTTTTTAATTTGTCTAATTCATTAAGTAAATATCCATCCATTAATTCTTCCCAT #########C@;(-A;(;663,3)?<EC=77==)@@8)@FC=)=8==??8?0*@CCFB4B:*B:93:FD3IIIIG NH:i:2 HI:i:2 AS:i:139 nM:i:1 MD:Z:68
  53. --- 307047,307056 ----
  54. FCC4MT3ACXX:7:2105:5964:7144# 339 PRELSG_13_v1 1170699 3 70M5S = 1170532 -237 TTCATCGGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCGTCGCCCAT CCBDDDDDDDDCADDDDDDDEFDCCDDDDDDCDDDEEEDEFFFFFFFGHHHHJJJJJJIIHEJIJJJJJJJJJIJ NH:i:2 HI:i:2 AS:i:141 nM:i:1 MD:Z:66A3
  55. FCC4MT3ACXX:7:2111:4162:2811# 99 PRELSG_13_v1 1170699 3 5S70M = 1170887 263 TTATCTTCATCTGTTTCACTTTCTTCAATATCTTCTTTTCCATCTTCAATAATTTCTTCATTATCTTTTTCATCG JJIGIIIJIIJJIHHJIEGGHIIJJIG@EDHIJIGIIIIJJIJJJIGGHGIIIIHGIGIIBHEHFHFHHFFDDDD NH:i:2 HI:i:1 AS:i:129 nM:i:7 MD:Z:6G4T22T10C24
  56. FCC4MT3ACXX:7:2313:16909:82298# 403 PRELSG_13_v1 1170699 3 70M5S = 1170587 -182 TTCATCGGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCACCGCCCAT ::<<9B<@:CA:+4(DDDDDCCA@CA?9?BA>@@DC>;>>;BA@CBC@HCA7?774GIIIIGCB-@FHDBGCIIH NH:i:2 HI:i:2 AS:i:141 nM:i:1 MD:Z:67T2
  57. ! FCC4MT3ACXX:7:2115:15127:71640# 163 PRELSG_13_v1 1170704 3 74M1S = 1170928 294 CGGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTGTTTCACCGGTTTCATTTT JIJD@HEHGHIJI<BFFIJJI@FIEHEFGGHIJJIJJIDHHIJIJJJIIIIIIJII4CEEEHHHEF=ACCEEEFE NH:i:2 HI:i:1 AS:i:135 nM:i:3 MD:Z:56T5T6�0T3
  58. ! FCC4MT3ACXX:7:1202:14259:28598# 403 PRELSG_13_v1 1170705 1 70M5S = 1170680 -95 GGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCATCGTTATTTCCCAT BDCDCA>ACCDDDDEEEDDEDEDFFFFDEFGGHAFIGGGEGIIIIIIIHIJJJIJJJIIICJIGFDGEGFGDHGG NH:i:3 HI:i:2 AS:i:138 nM:i:2 MD:Z:64G1T1�1
  59. ! FCC4MT3ACXX:7:1202:14259:28598# 403 PRELSG_13_v1 1170705 1 70M5S = 1170617 -158 GGTTTTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCATCGTTATTTCCCAT BDCDCA>ACCDDDDEEEDDEDEDFFFFDEFGGHAFIGGGEGIIIIIIIHIJJJIJJJIIICJIGFDGEGFGDHGG NH:i:3 HI:i:3 AS:i:138 nM:i:2 MD:Z:64G1T1�1
  60. ! FCC4MT3ACXX:7:2115:17199:58163# 339 PRELSG_13_v1 1170709 1 69M6S = 1170646 -132 TTACTTTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCACCGGTTTCATTTTCCCAT C>4>(CCC@>AEDCCCCA?@BCCAACECFFFFFFHHGHHHIIIGIGIHEGGHGGEHGEGDD?4@FC9::)?;@HA NH:i:3 HI:i:3 AS:i:137 nM:i:2 MD:Z:57T6�0T3
  61. FCC4MT3ACXX:7:2209:4641:95092# 99 PRELSG_13_v1 1170714 1 9M158N66M = 1170950 311 TTCTTCAATATCTTCTTTTTCATCTTCAATCATTTCTTCATTATCTTTTTCACCGGTTTCATTTTCTTTCTTTTC IJJJIIIEIBGGJJJJIJJIJJGIJJJGGHIHIIIJIJJIHHJJJIJJJIGIGGIE=BEFDEDEEEEEEEDDDDD NH:i:3 HI:i:1 AS:i:136 nM:i:2 MD:Z:75
  62. FCC4MT3ACXX:7:2113:15881:6256# 339 PRELSG_13_v1 1170780 3 70M5S = 1170305 -545 AGTCTTTTTTCATTTTCATTGTTTTTTAATTTGTCTAATTCATTAAGTAAATATCCATCCATTAATTCTTCCCAT CCB@B?CCCCCCC>C@CC=9<>?=7>CEE@;BHEGGEGIHGGHGGHGFDGCIDHIGHEFBFGGGDF>GD;CCHGG NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:70
  63. FCC4MT3ACXX:7:2113:15894:6267# 339 PRELSG_13_v1 1170782 3 2S68M5S = 1170305 -545 ATTCTTTTTTCATTTTCATTGTTTTTTAATTTGTCTAATTCATTAAGTAAATATCCATCCATTAATTCTTCCCAT #########C@;(-A;(;663,3)?<EC=77==)@@8)@FC=)=8==??8?0*@CCFB4B:*B:93:FD3IIIIG NH:i:2 HI:i:2 AS:i:139 nM:i:1 MD:Z:68
  64. ***************
  65. *** 351784,351806 ****
  66. FCC4MT3ACXX:7:2202:12691:76822# 403 PRELSG_14_v1 538183 1 75M = 536529 -1729 ATGATGTATATGCTTGGTTAAAAAAAGCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGG CDCADDECDCDBB@CDCCDDFHHCIJJJJIIJJGJJJIIIGIHF<IIIGFIGCIJIIIIJJJJHHGIHHIHGJJI NH:i:3 HI:i:3 AS:i:132 nM:i:5 MD:Z:75
  67. FCC4MT3ACXX:7:1207:4218:34543# 339 PRELSG_14_v1 538191 1 75M = 538116 -150 TATGCTTGGTTAAAAAAAGCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATT DCDDBBDDDCDDDFDFFFHFHHHHHHCC;CGEGHHGCEHIJJJJJJJIIFJIIIHGJJJHGIHDJJJJJIGIJIH NH:i:3 HI:i:2 AS:i:111 nM:i:2 MD:Z:75
  68. FCC4MT3ACXX:7:1207:4218:34543# 339 PRELSG_14_v1 538191 1 75M = 536532 -1734 TATGCTTGGTTAAAAAAAGCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATT DCDDBBDDDCDDDFDFFFHFHHHHHHCC;CGEGHHGCEHIJJJJJJJIIFJIIIHGJJJHGIHDJJJJJIGIJIH NH:i:3 HI:i:3 AS:i:110 nM:i:2 MD:Z:75
  69. ! FCC4MT3ACXX:7:1108:11507:52888# 339 PRELSG_14_v1 538209 3 75M = 538119 -165 GCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATTGTAAGAATATTAAAAAAC CDECEDEECFCFDDFHHHAE<HIHEIHEJIGHGGIIHIIGHHEGJIIIJGHJGJJJJIIHIJIIIJIIJJJJIHJ NH:i:2 HI:i:2 AS:i:128 nM:i:7 MD:Z:704
  70. ! FCC4MT3ACXX:7:2202:14122:7950# 419 PRELSG_14_v1 538226 1 5S70M = 538310 159 ATGGGTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTAT JJJJIGHJIJIJJJHIIIJEIGHHIIJHIJJIIIIJJFHHHHHFFFFFFEEEEEEFEEDDDDDDDDDDDCCCDDD NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:5316
  71. ! FCC4MT3ACXX:7:1111:16766:58722# 355 PRELSG_14_v1 538249 1 75M = 538313 139 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC JIJJJJJJJIIJJJJJJJHJJJJJJJJJJJIJJJJJIHHHHHHFFFFFFFCCCCEEDCDDDDDDDDDDDD>ACDE NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  72. ! FCC4MT3ACXX:7:1115:13546:38280# 419 PRELSG_14_v1 538249 1 75M = 538296 122 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC IJJJJJJJJIIJJJJJJJGGEHIJJIIJJJJJJJJJJHHHHHHFFFFFFFEEEEEEDDDDDDCCDDDCDD:@CDE NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  73. ! FCC4MT3ACXX:7:1204:16908:41047# 419 PRELSG_14_v1 538249 1 75M = 538380 206 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC BAF4CE9C??60?GHCGCBD?8BGEFFAGH<F@EBACCACEEEC:>BBBDCC3@CED3>?CC>CCA>>CB##### NH:i:3 HI:i:2 AS:i:145 nM:i:1 MD:Z:3044
  74. ! FCC4MT3ACXX:7:1311:3395:37365# 419 PRELSG_14_v1 538249 1 75M = 538374 200 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC FEFFHGBHEG?DHG@DDGFFHD@D8>BBFGGIIIH3CA;CHAEB@B@CEFACCCCC>CD>@CDCCC5>C###### NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  75. ! FCC4MT3ACXX:7:1311:5482:46840# 419 PRELSG_14_v1 538249 1 75M = 538332 158 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC JIGGGHGIGHGHIIIEIE?BGIEHGEHIEHHIIJGAHHFGGHFFFDDC@DCCEDEEACDDDDDC@C>ACDCADDC NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  76. ! FCC4MT3ACXX:7:2104:13546:49655# 419 PRELSG_14_v1 538249 1 75M = 538297 123 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC ?@DEGB?FBBHAH@*90B9?BB94B8=F==FF@GBHGECCCAABCBDFED:;;>;(;>CD>CDDCCDCCA5:@AD NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  77. ! FCC4MT3ACXX:7:2107:12862:5600# 355 PRELSG_14_v1 538249 1 75M = 538350 176 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC :?CCHIGIBF?@FHHIIB?DGHEBFGG98C<FAAEGACEAACCC33?BD@>;>CCA>A@CAACC>>::<?((;>C NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  78. ! FCC4MT3ACXX:7:2111:4444:49407# 419 PRELSG_14_v1 538249 1 75M = 538298 124 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC EF9ECGDBH<?F@9??FD*8BF>DDF@DGGHICIEAA>?EAHFBCCDDCDC:CCCEA@CD@ACCACCA<A>>CDD NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  79. ! FCC4MT3ACXX:7:2111:8495:98708# 355 PRELSG_14_v1 538249 1 75M = 538316 142 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC IJIJJJIJJFHJJJJJJIFIIIIGHHGJIJFHGJIGEHHEHHHFFFFDDDCE>@CECDCCDCCCCDDDDD:>CDA NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  80. ! FCC4MT3ACXX:7:2209:3291:95633# 419 PRELSG_14_v1 538249 1 75M = 538331 157 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC EFHGCGHGIDHIJJJJJIHIIIJJJHIJIIJJJJJEIFFGHHHFFFFFFFCCEEEEDDDDCAADDDDC?A5>ACD NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  81. ! FCC4MT3ACXX:7:2310:19637:45515# 355 PRELSG_14_v1 538249 1 75M = 538328 154 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC EHEDDEFGGDAFD?BFDHFF8B4?BBGHICE@;BAC:AH>CEBBB3;@@A>AC>>ACCDCDDDD@@A(9?C(;;> NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:3044
  82. ! FCC4MT3ACXX:7:1109:12427:95030# 419 PRELSG_14_v1 538250 1 5S70M = 538322 147 ATGGGTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGT IJHJJ?DHIIJIIIBHGH<FIHDHIFHIIJIJJIDHIGHHFB)7?CCE>CEDDECCDDDDDDDDDDDDDDDCDD4 NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:2940
  83. ! FCC4MT3ACXX:7:2113:8515:32041# 355 PRELSG_14_v1 538250 1 5S70M = 538303 128 ATGGGTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGT JJJJJ:DGHGIIJHHHIJJIJJHIIIJIJJIGIHIGIHHHHFFFEFFFEEEEEECCCDDDCDDDDCCCDDDCCD@ NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:2940
  84. ! FCC4MT3ACXX:7:2311:17816:71982# 355 PRELSG_14_v1 538251 1 5S70M = 538350 174 ATGGGACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTA ?GEGIIGEGGHIIAHEH>DGGGHGEHIIHGGEAFIGEHE>BC?CDDECEECEECCCCCCCCCCCAC@:>:AC?>> NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:2841
  85. ! FCC4MT3ACXX:7:2213:3478:49379# 419 PRELSG_14_v1 538256 1 5S70M = 538302 121 ATGGGGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATACAC GFHGGJIJIJIGIGFGDDHBFHGCFGFGIJIHH@HEFFF>CBCC>CCCCCCDCD>@CCC@ACCC9ACB::@CDDD NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:2346
  86. FCC4MT3ACXX:7:1115:13546:38280# 339 PRELSG_14_v1 538296 1 75M = 538249 -122 ATAGAAAGATCACTGCAAAGAGGTATACACCCGAATGAGTTGAATATAGGTGTAACAAAAGCACAATCAATTAAA DDDDDCDDEDDDDEDDDCDDDEFECECDBEBHHJJIIJIJIGJIJJJJJJIJJJJJJJJIJJIJJJJIJJJIIII NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:75
  87. FCC4MT3ACXX:7:1115:13546:38280# 339 PRELSG_14_v1 538296 1 75M = 536665 -1706 ATAGAAAGATCACTGCAAAGAGGTATACACCCGAATGAGTTGAATATAGGTGTAACAAAAGCACAATCAATTAAA DDDDDCDDEDDDDEDDDCDDDEFECECDBEBHHJJIIJIJIGJIJJJJJJIJJJJJJJJIJJIJJJJIJJJIIII NH:i:3 HI:i:3 AS:i:147 nM:i:0 MD:Z:75
  88. FCC4MT3ACXX:7:2104:13546:49655# 339 PRELSG_14_v1 538297 1 75M = 538249 -123 TAGAAAGATCACTGCAAAGAGGTATACACCCGAATGAGTTGAATATAGGTGTAACAAAAGCACAATCAATTAAAA ###########@;ABA;5;5(;6(6/B?4A=A77=C=@=)4=CF@@;==)C=/(994?99*4GB?9BD<AFDBD< NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:75
  89. --- 351784,351806 ----
  90. FCC4MT3ACXX:7:2202:12691:76822# 403 PRELSG_14_v1 538183 1 75M = 536529 -1729 ATGATGTATATGCTTGGTTAAAAAAAGCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGG CDCADDECDCDBB@CDCCDDFHHCIJJJJIIJJGJJJIIIGIHF<IIIGFIGCIJIIIIJJJJHHGIHHIHGJJI NH:i:3 HI:i:3 AS:i:132 nM:i:5 MD:Z:75
  91. FCC4MT3ACXX:7:1207:4218:34543# 339 PRELSG_14_v1 538191 1 75M = 538116 -150 TATGCTTGGTTAAAAAAAGCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATT DCDDBBDDDCDDDFDFFFHFHHHHHHCC;CGEGHHGCEHIJJJJJJJIIFJIIIHGJJJHGIHDJJJJJIGIJIH NH:i:3 HI:i:2 AS:i:111 nM:i:2 MD:Z:75
  92. FCC4MT3ACXX:7:1207:4218:34543# 339 PRELSG_14_v1 538191 1 75M = 536532 -1734 TATGCTTGGTTAAAAAAAGCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATT DCDDBBDDDCDDDFDFFFHFHHHHHHCC;CGEGHHGCEHIJJJJJJJIIFJIIIHGJJJHGIHDJJJJJIGIJIH NH:i:3 HI:i:3 AS:i:110 nM:i:2 MD:Z:75
  93. ! FCC4MT3ACXX:7:1108:11507:52888# 339 PRELSG_14_v1 538209 3 75M = 538119 -165 GCAGGAAAATTTAAAGCTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATTGTAAGAATATTAAAAAAC CDECEDEECFCFDDFHHHAE<HIHEIHEJIGHGGIIHIIGHHEGJIIIJGHJGJJJJIIHIJIIIJIIJJJJIHJ NH:i:2 HI:i:2 AS:i:128 nM:i:7 MD:Z:70�4
  94. ! FCC4MT3ACXX:7:2202:14122:7950# 419 PRELSG_14_v1 538226 1 5S70M = 538310 159 ATGGGTACTCAGAGAACAGAAGGAGTATCTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTAT JJJJIGHJIJIJJJHIIIJEIGHHIIJHIJJIIIIJJFHHHHHFFFFFFEEEEEEFEEDDDDDDDDDDDCCCDDD NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:53�16
  95. ! FCC4MT3ACXX:7:1111:16766:58722# 355 PRELSG_14_v1 538249 1 75M = 538313 139 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC JIJJJJJJJIIJJJJJJJHJJJJJJJJJJJIJJJJJIHHHHHHFFFFFFFCCCCEEDCDDDDDDDDDDDD>ACDE NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  96. ! FCC4MT3ACXX:7:1115:13546:38280# 419 PRELSG_14_v1 538249 1 75M = 538296 122 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC IJJJJJJJJIIJJJJJJJGGEHIJJIIJJJJJJJJJJHHHHHHFFFFFFFEEEEEEDDDDDDCCDDDCDD:@CDE NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  97. ! FCC4MT3ACXX:7:1204:16908:41047# 419 PRELSG_14_v1 538249 1 75M = 538380 206 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC BAF4CE9C??60?GHCGCBD?8BGEFFAGH<F@EBACCACEEEC:>BBBDCC3@CED3>?CC>CCA>>CB##### NH:i:3 HI:i:2 AS:i:145 nM:i:1 MD:Z:30�44
  98. ! FCC4MT3ACXX:7:1311:3395:37365# 419 PRELSG_14_v1 538249 1 75M = 538374 200 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC FEFFHGBHEG?DHG@DDGFFHD@D8>BBFGGIIIH3CA;CHAEB@B@CEFACCCCC>CD>@CDCCC5>C###### NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  99. ! FCC4MT3ACXX:7:1311:5482:46840# 419 PRELSG_14_v1 538249 1 75M = 538332 158 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC JIGGGHGIGHGHIIIEIE?BGIEHGEHIEHHIIJGAHHFGGHFFFDDC@DCCEDEEACDDDDDC@C>ACDCADDC NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  100. ! FCC4MT3ACXX:7:2104:13546:49655# 419 PRELSG_14_v1 538249 1 75M = 538297 123 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC ?@DEGB?FBBHAH@*90B9?BB94B8=F==FF@GBHGECCCAABCBDFED:;;>;(;>CD>CDDCCDCCA5:@AD NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  101. ! FCC4MT3ACXX:7:2107:12862:5600# 355 PRELSG_14_v1 538249 1 75M = 538350 176 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC :?CCHIGIBF?@FHHIIB?DGHEBFGG98C<FAAEGACEAACCC33?BD@>;>CCA>A@CAACC>>::<?((;>C NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  102. ! FCC4MT3ACXX:7:2111:4444:49407# 419 PRELSG_14_v1 538249 1 75M = 538298 124 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC EF9ECGDBH<?F@9??FD*8BF>DDF@DGGHICIEAA>?EAHFBCCDDCDC:CCCEA@CD@ACCACCA<A>>CDD NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  103. ! FCC4MT3ACXX:7:2111:8495:98708# 355 PRELSG_14_v1 538249 1 75M = 538316 142 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC IJIJJJIJJFHJJJJJJIFIIIIGHHGJIJFHGJIGEHHEHHHFFFFDDDCE>@CECDCCDCCCCDDDDD:>CDA NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  104. ! FCC4MT3ACXX:7:2209:3291:95633# 419 PRELSG_14_v1 538249 1 75M = 538331 157 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC EFHGCGHGIDHIJJJJJIHIIIJJJHIJIIJJJJJEIFFGHHHFFFFFFFCCEEEEDDDDCAADDDDC?A5>ACD NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  105. ! FCC4MT3ACXX:7:2310:19637:45515# 355 PRELSG_14_v1 538249 1 75M = 538328 154 CTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATAC EHEDDEFGGDAFD?BFDHFF8B4?BBGHICE@;BAC:AH>CEBBB3;@@A>AC>>ACCDCDDDD@@A(9?C(;;> NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:30�44
  106. ! FCC4MT3ACXX:7:1109:12427:95030# 419 PRELSG_14_v1 538250 1 5S70M = 538322 147 ATGGGTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGT IJHJJ?DHIIJIIIBHGH<FIHDHIFHIIJIJJIDHIGHHFB)7?CCE>CEDDECCDDDDDDDDDDDDDDDCDD4 NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:29�40
  107. ! FCC4MT3ACXX:7:2113:8515:32041# 355 PRELSG_14_v1 538250 1 5S70M = 538303 128 ATGGGTACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGT JJJJJ:DGHGIIJHHHIJJIJJHIIIJIJJIGIHIGIHHHHFFFEFFFEEEEEECCCDDDCDDDDCCCDDDCCD@ NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:29�40
  108. ! FCC4MT3ACXX:7:2311:17816:71982# 355 PRELSG_14_v1 538251 1 5S70M = 538350 174 ATGGGACAACGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTA ?GEGIIGEGGHIIAHEH>DGGGHGEHIIHGGEAFIGEHE>BC?CDDECEECEECCCCCCCCCCCAC@:>:AC?>> NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:28�41
  109. ! FCC4MT3ACXX:7:2213:3478:49379# 419 PRELSG_14_v1 538256 1 5S70M = 538302 121 ATGGGGGATTTAATTGTAAGAATATTAAAAAACTATGAAGATTATATAGAAAGATCACTGCAAAGAGGTATACAC GFHGGJIJIJIGIGFGDDHBFHGCFGFGIJIHH@HEFFF>CBCC>CCCCCCDCD>@CCC@ACCC9ACB::@CDDD NH:i:3 HI:i:2 AS:i:142 nM:i:0 MD:Z:23�46
  110. FCC4MT3ACXX:7:1115:13546:38280# 339 PRELSG_14_v1 538296 1 75M = 538249 -122 ATAGAAAGATCACTGCAAAGAGGTATACACCCGAATGAGTTGAATATAGGTGTAACAAAAGCACAATCAATTAAA DDDDDCDDEDDDDEDDDCDDDEFECECDBEBHHJJIIJIJIGJIJJJJJJIJJJJJJJJIJJIJJJJIJJJIIII NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:75
  111. FCC4MT3ACXX:7:1115:13546:38280# 339 PRELSG_14_v1 538296 1 75M = 536665 -1706 ATAGAAAGATCACTGCAAAGAGGTATACACCCGAATGAGTTGAATATAGGTGTAACAAAAGCACAATCAATTAAA DDDDDCDDEDDDDEDDDCDDDEFECECDBEBHHJJIIJIJIGJIJJJJJJIJJJJJJJJIJJIJJJJIJJJIIII NH:i:3 HI:i:3 AS:i:147 nM:i:0 MD:Z:75
  112. FCC4MT3ACXX:7:2104:13546:49655# 339 PRELSG_14_v1 538297 1 75M = 538249 -123 TAGAAAGATCACTGCAAAGAGGTATACACCCGAATGAGTTGAATATAGGTGTAACAAAAGCACAATCAATTAAAA ###########@;ABA;5;5(;6(6/B?4A=A77=C=@=)4=CF@@;==)C=/(994?99*4GB?9BD<AFDBD< NH:i:3 HI:i:2 AS:i:147 nM:i:0 MD:Z:75
  113. ***************
  114. *** 361039,361059 ****
  115. FCC4MT3ACXX:7:1115:2199:74558# 419 PRELSG_14_v1 1344538 0 75M = 1344748 285 TATTATTAAAATAAAATAGAGATAAAAGCGAAAAAAATGATAAAAAATTAAATTATAAAAATTATAAATTTAATT :E4,ACFFH4CB+++1@*:**:9?*:9?B)8@<((@/@7DCH3=AHDB(;>:;>C@CD3@3;::@########## NH:i:7 HI:i:5 AS:i:140 nM:i:4 MD:Z:29T8T9G21G4
  116. FCC4MT3ACXX:7:1115:2199:74558# 419 PRELSG_14_v1 1344538 0 75M = 1344749 286 TATTATTAAAATAAAATAGAGATAAAAGCGAAAAAAATGATAAAAAATTAAATTATAAAAATTATAAATTTAATT :E4,ACFFH4CB+++1@*:**:9?*:9?B)8@<((@/@7DCH3=AHDB(;>:;>C@CD3@3;::@########## NH:i:7 HI:i:6 AS:i:140 nM:i:4 MD:Z:29T8T9G21G4
  117. FCC4MT3ACXX:7:1115:2199:74558# 419 PRELSG_14_v1 1344538 0 75M = 1344750 287 TATTATTAAAATAAAATAGAGATAAAAGCGAAAAAAATGATAAAAAATTAAATTATAAAAATTATAAATTTAATT :E4,ACFFH4CB+++1@*:**:9?*:9?B)8@<((@/@7DCH3=AHDB(;>:;>C@CD3@3;::@########## NH:i:7 HI:i:7 AS:i:140 nM:i:4 MD:Z:29T8T9G21G4
  118. ! FCC4MT3ACXX:7:2304:9891:48808# 147 PRELSG_14_v1 1344575 255 75M = 1344534 -116 TTATAAAAAATGAAATTATAAAAATTATAAATTGAATTTAGTAAAAAGAAGAAAAAGGAGAAAAAAGAAAAAAAA EECDDDEEEEEDEDEDDDDDDEEEEEFDDEEEEEEECCEFFFFFFGHHHHHIGDIJJJIJIJJJJJJJJJJJJJI NH:i:1 HI:i:1 AS:i:139 nM:i:4 MD:Z:4114A0A1A6A8
  119. ! FCC4MT3ACXX:7:1113:15419:69479# 163 PRELSG_14_v1 1344614 0 4S71M = 1344744 205 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:1 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  120. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344745 206 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:2 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  121. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344746 207 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:3 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  122. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344747 208 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:4 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  123. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344748 209 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:5 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  124. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344749 210 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:6 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  125. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344750 211 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:7 AS:i:123 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A32
  126. ! FCC4MT3ACXX:7:2101:20957:58520# 99 PRELSG_14_v1 1344614 0 9S66M = 1344744 205 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:1 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  127. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344745 206 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:2 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  128. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344746 207 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:3 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  129. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344747 208 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:4 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  130. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344748 209 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:5 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  131. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344749 210 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:6 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  132. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344750 211 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:7 AS:i:118 nM:i:10 MD:Z:23A4G6A1A1A4A1A2A4A0A27
  133. FCC4MT3ACXX:7:1309:19594:11678# 355 PRELSG_14_v1 1344617 0 1S68M6S = 1344738 194 AAATATGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTTCA IIJJJJJJJJJJJJIJIJIJHFDDDDBDDDDDDDDDDDDDDDDDDBBBBDBBBDDDDDDDBDDBB########## NH:i:9 HI:i:7 AS:i:131 nM:i:4 MD:Z:2A1A32G30
  134. FCC4MT3ACXX:7:1104:2104:61679# 355 PRELSG_14_v1 1344618 3 9S66M = 1344743 200 CTGAAGAAGAAAAAAAGAAAAAAAACAGACAAATAAGTAGAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA EIIHEC@HDFIIGGDHGBHDEEGHFFFECCBAC35>A@CC:>CCCDDCDDBDD@<@BBDBDDD@<359>BBD955 NH:i:2 HI:i:2 AS:i:121 nM:i:9 MD:Z:4G11A1A1A3A2A0A1A4A30
  135. FCC4MT3ACXX:7:1205:7103:37898# 355 PRELSG_14_v1 1344618 1 20S55M = 1344659 110 AAGGGCACTTGCTGAAGAAGAAAAAAAGAAAAAAAACAGACAAATAAGTAGAAAATGAAAAAAAAAAAAAAAAAA JJJJJJJJGIJJIJIJIJJIHGHJJJJJJJJIGHDDDDDDDDDDDDDDBDCCDDDDDDDDDBBDDDB>BBBDDDD NH:i:4 HI:i:2 AS:i:102 nM:i:10 MD:Z:4G11A1A1A3A2A0A1A4A19
  136. --- 361039,361059 ----
  137. FCC4MT3ACXX:7:1115:2199:74558# 419 PRELSG_14_v1 1344538 0 75M = 1344748 285 TATTATTAAAATAAAATAGAGATAAAAGCGAAAAAAATGATAAAAAATTAAATTATAAAAATTATAAATTTAATT :E4,ACFFH4CB+++1@*:**:9?*:9?B)8@<((@/@7DCH3=AHDB(;>:;>C@CD3@3;::@########## NH:i:7 HI:i:5 AS:i:140 nM:i:4 MD:Z:29T8T9G21G4
  138. FCC4MT3ACXX:7:1115:2199:74558# 419 PRELSG_14_v1 1344538 0 75M = 1344749 286 TATTATTAAAATAAAATAGAGATAAAAGCGAAAAAAATGATAAAAAATTAAATTATAAAAATTATAAATTTAATT :E4,ACFFH4CB+++1@*:**:9?*:9?B)8@<((@/@7DCH3=AHDB(;>:;>C@CD3@3;::@########## NH:i:7 HI:i:6 AS:i:140 nM:i:4 MD:Z:29T8T9G21G4
  139. FCC4MT3ACXX:7:1115:2199:74558# 419 PRELSG_14_v1 1344538 0 75M = 1344750 287 TATTATTAAAATAAAATAGAGATAAAAGCGAAAAAAATGATAAAAAATTAAATTATAAAAATTATAAATTTAATT :E4,ACFFH4CB+++1@*:**:9?*:9?B)8@<((@/@7DCH3=AHDB(;>:;>C@CD3@3;::@########## NH:i:7 HI:i:7 AS:i:140 nM:i:4 MD:Z:29T8T9G21G4
  140. ! FCC4MT3ACXX:7:2304:9891:48808# 147 PRELSG_14_v1 1344575 255 75M = 1344534 -116 TTATAAAAAATGAAATTATAAAAATTATAAATTGAATTTAGTAAAAAGAAGAAAAAGGAGAAAAAAGAAAAAAAA EECDDDEEEEEDEDEDDDDDDEEEEEFDDEEEEEEECCEFFFFFFGHHHHHIGDIJJJIJIJJJJJJJJJJJJJI NH:i:1 HI:i:1 AS:i:139 nM:i:4 MD:Z:41�14A0A1A6A8
  141. ! FCC4MT3ACXX:7:1113:15419:69479# 163 PRELSG_14_v1 1344614 0 4S71M = 1344744 205 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:1 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  142. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344745 206 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:2 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  143. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344746 207 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:3 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  144. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344747 208 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:4 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  145. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344748 209 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:5 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  146. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344749 210 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:6 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  147. ! FCC4MT3ACXX:7:1113:15419:69479# 419 PRELSG_14_v1 1344614 0 4S71M = 1344750 211 AGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA IGGIJIIJJGGIIIGIIIIJJFHEECEEFFFFEEEEDEDDDDDDDDDDDDDBBBDDDDDDDDDDBDBBDDDBDBB NH:i:7 HI:i:7 AS:i:123 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A32
  148. ! FCC4MT3ACXX:7:2101:20957:58520# 99 PRELSG_14_v1 1344614 0 9S66M = 1344744 205 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:1 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  149. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344745 206 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:2 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  150. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344746 207 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:3 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  151. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344747 208 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:4 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  152. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344748 209 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:5 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  153. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344749 210 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:6 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  154. ! FCC4MT3ACXX:7:2101:20957:58520# 355 PRELSG_14_v1 1344614 0 9S66M = 1344750 211 GAGAATGACAGAAAATAGAACAAAAAAGAGAGAAAATAGAAGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA HFHGIJJJJJJJJJJJJJIJJJJJJJHHFFFFDEEEEDDDCCDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDD NH:i:7 HI:i:7 AS:i:118 nM:i:10 MD:Z:2�3A4G6A1A1A4A1A2A4A0A27
  155. FCC4MT3ACXX:7:1309:19594:11678# 355 PRELSG_14_v1 1344617 0 1S68M6S = 1344738 194 AAATATGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTTCA IIJJJJJJJJJJJJIJIJIJHFDDDDBDDDDDDDDDDDDDDDDDDBBBBDBBBDDDDDDDBDDBB########## NH:i:9 HI:i:7 AS:i:131 nM:i:4 MD:Z:2A1A32G30
  156. FCC4MT3ACXX:7:1104:2104:61679# 355 PRELSG_14_v1 1344618 3 9S66M = 1344743 200 CTGAAGAAGAAAAAAAGAAAAAAAACAGACAAATAAGTAGAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA EIIHEC@HDFIIGGDHGBHDEEGHFFFECCBAC35>A@CC:>CCCDDCDDBDD@<@BBDBDDD@<359>BBD955 NH:i:2 HI:i:2 AS:i:121 nM:i:9 MD:Z:4G11A1A1A3A2A0A1A4A30
  157. FCC4MT3ACXX:7:1205:7103:37898# 355 PRELSG_14_v1 1344618 1 20S55M = 1344659 110 AAGGGCACTTGCTGAAGAAGAAAAAAAGAAAAAAAACAGACAAATAAGTAGAAAATGAAAAAAAAAAAAAAAAAA JJJJJJJJGIJJIJIJIJJIHGHJJJJJJJJIGHDDDDDDDDDDDDDDBDCCDDDDDDDDDBBDDDB>BBBDDDD NH:i:4 HI:i:2 AS:i:102 nM:i:10 MD:Z:4G11A1A1A3A2A0A1A4A19
  158. ***************
  159. *** 522217,522224 ****
  160. FCC4MT3ACXX:7:1303:16317:30039# 147 PRELSG_00_v1_archived_contig_323 391 255 70M5S = 320 -141 CAATAGCACTAAATGTTTCCTTAATTTTCTCTTCACTTAGTGAACTATGAAATTTTTTTACCATTGATCTCCCAA CCA>5>:,C@D@:DDCDC;;CCCCEDDEEFFEDB;DHFEHHGHDJIGHFFFBGHEIHD?0HGFGHGGIJIIJIIG NH:i:1 HI:i:1 AS:i:143 nM:i:0 MD:Z:70
  161. FCC4MT3ACXX:7:2107:16674:27052# 99 PRELSG_00_v1_archived_contig_323 410 3 75M = 495 155 CTTAATTTTCTCTTCACTTAGTGAACTATGAAATTTTTTTACCATTGATCTGATATTTTCACTGTTCACATCAAC JJJJJJIJJJJJJJJIJJJJJGIFHIIFIJJIIIJJJJIJIGHGGIJIJHAEHEFFFFFFFFFDCEEDCEDDCCD NH:i:2 HI:i:2 AS:i:142 nM:i:0 MD:Z:75
  162. FCC4MT3ACXX:7:2204:3293:31223# 403 PRELSG_00_v1_archived_contig_323 437 3 70M5S = 354 -153 ATGAAATTTTTTTACCATTGATCTGATATTTTCACTGTTCACATCAACATTATATTTAGTTTGTACATAACCCAT DDACCDDDDEDC@@FFFFFEHHEHFHIHJJJIHCIJIIIHCHFGIHGHGFGJJIJIJIJJJIHFFIGGC:JJIIJ NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:70
  163. ! FCC4MT3ACXX:7:2303:6266:29057# 99 PRELSG_00_v1_archived_contig_323 481 3 75M = 564 154 CAACATTATATTTAGTTTGTACATAAGCTTTAAATAAATCTGAGGATTTTTTTATATCTTCTTTTTCTTGTAAAA JIJIGIJJJJJJIJJCGGHFHJJIIJJIIHIIGHHIHJGHIJHGGGGIIJHGEHHEFDFEDBDCCCCEECA;>;@ NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:4628
  164. ! FCC4MT3ACXX:7:2107:16674:27052# 147 PRELSG_00_v1_archived_contig_323 495 3 70M5S = 410 -155 GTTTGTACATAAGCTTTAAATAAATCTGAGGATTTTTTTATATCTTCTTTTTCTTGTAAAAATGATTTATCCCAT DDDEDCCDCA>5CDDCEEDFCDDDCDDEEECEDHJIJIIJJJJJIHHEB9JIIIIIIJGJJHIGIJIIHHJJHII NH:i:2 HI:i:2 AS:i:142 nM:i:0 MD:Z:3237
  165. FCC4MT3ACXX:7:1307:5059:82634# 99 PRELSG_00_v1_archived_contig_323 533 3 75M = 600 138 TATATCTTCTTTTTCTTGTAAAAATGATTTATGTATAATATCTAAAATTGGATTCTTAAATTTTCCCTCGTACCA IGIGIGIIIJJJIJJIJJIJJJJJIJGIIJJJJHJIIGICHGHHCEHJJJDFFHFEFEFFFECEDCEDDDDDB@D NH:i:2 HI:i:1 AS:i:144 nM:i:0 MD:Z:75
  166. FCC4MT3ACXX:7:2303:6266:29057# 147 PRELSG_00_v1_archived_contig_323 564 3 71M4S = 481 -154 TGTATAATATCTAAAATTGGATTCTTAAATTTTCCCTCGTACCAAGTTTTCACATCTTTTAACATAGATTCCCCA :CCBA@DD@CDDCC@;355:@>>@EDEECDB;9AE@DGHCCIIGHHCHF>FDIIIGGJIHGFEHDD:?:1IGHEF NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:71
  167. FCC4MT3ACXX:7:1307:5059:82634# 147 PRELSG_00_v1_archived_contig_323 600 3 71M4S = 533 -138 TCGTACCAAGTTTTCACATCTTTTAACATAGATTCATTTTTAAAACTATATGTTTCAAGTACACATATAATCCCA B@C>CCCDDEEDEA@@CB?@CC=)A;HEG@;JIGHAGIGGHIIHFIGICGHAHEBIIIHFBFBFBD??*F?BFGG NH:i:2 HI:i:1 AS:i:144 nM:i:0 MD:Z:71
  168. --- 522217,522224 ----
  169. FCC4MT3ACXX:7:1303:16317:30039# 147 PRELSG_00_v1_archived_contig_323 391 255 70M5S = 320 -141 CAATAGCACTAAATGTTTCCTTAATTTTCTCTTCACTTAGTGAACTATGAAATTTTTTTACCATTGATCTCCCAA CCA>5>:,C@D@:DDCDC;;CCCCEDDEEFFEDB;DHFEHHGHDJIGHFFFBGHEIHD?0HGFGHGGIJIIJIIG NH:i:1 HI:i:1 AS:i:143 nM:i:0 MD:Z:70
  170. FCC4MT3ACXX:7:2107:16674:27052# 99 PRELSG_00_v1_archived_contig_323 410 3 75M = 495 155 CTTAATTTTCTCTTCACTTAGTGAACTATGAAATTTTTTTACCATTGATCTGATATTTTCACTGTTCACATCAAC JJJJJJIJJJJJJJJIJJJJJGIFHIIFIJJIIIJJJJIJIGHGGIJIJHAEHEFFFFFFFFFDCEEDCEDDCCD NH:i:2 HI:i:2 AS:i:142 nM:i:0 MD:Z:75
  171. FCC4MT3ACXX:7:2204:3293:31223# 403 PRELSG_00_v1_archived_contig_323 437 3 70M5S = 354 -153 ATGAAATTTTTTTACCATTGATCTGATATTTTCACTGTTCACATCAACATTATATTTAGTTTGTACATAACCCAT DDACCDDDDEDC@@FFFFFEHHEHFHIHJJJIHCIJIIIHCHFGIHGHGFGJJIJIJIJJJIHFFIGGC:JJIIJ NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:70
  172. ! FCC4MT3ACXX:7:2303:6266:29057# 99 PRELSG_00_v1_archived_contig_323 481 3 75M = 564 154 CAACATTATATTTAGTTTGTACATAAGCTTTAAATAAATCTGAGGATTTTTTTATATCTTCTTTTTCTTGTAAAA JIJIGIJJJJJJIJJCGGHFHJJIIJJIIHIIGHHIHJGHIJHGGGGIIJHGEHHEFDFEDBDCCCCEECA;>;@ NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:46�28
  173. ! FCC4MT3ACXX:7:2107:16674:27052# 147 PRELSG_00_v1_archived_contig_323 495 3 70M5S = 410 -155 GTTTGTACATAAGCTTTAAATAAATCTGAGGATTTTTTTATATCTTCTTTTTCTTGTAAAAATGATTTATCCCAT DDDEDCCDCA>5CDDCEEDFCDDDCDDEEECEDHJIJIIJJJJJIHHEB9JIIIIIIJGJJHIGIJIIHHJJHII NH:i:2 HI:i:2 AS:i:142 nM:i:0 MD:Z:32�37
  174. FCC4MT3ACXX:7:1307:5059:82634# 99 PRELSG_00_v1_archived_contig_323 533 3 75M = 600 138 TATATCTTCTTTTTCTTGTAAAAATGATTTATGTATAATATCTAAAATTGGATTCTTAAATTTTCCCTCGTACCA IGIGIGIIIJJJIJJIJJIJJJJJIJGIIJJJJHJIIGICHGHHCEHJJJDFFHFEFEFFFECEDCEDDDDDB@D NH:i:2 HI:i:1 AS:i:144 nM:i:0 MD:Z:75
  175. FCC4MT3ACXX:7:2303:6266:29057# 147 PRELSG_00_v1_archived_contig_323 564 3 71M4S = 481 -154 TGTATAATATCTAAAATTGGATTCTTAAATTTTCCCTCGTACCAAGTTTTCACATCTTTTAACATAGATTCCCCA :CCBA@DD@CDDCC@;355:@>>@EDEECDB;9AE@DGHCCIIGHHCHF>FDIIIGGJIHGFEHDD:?:1IGHEF NH:i:2 HI:i:2 AS:i:143 nM:i:0 MD:Z:71
  176. FCC4MT3ACXX:7:1307:5059:82634# 147 PRELSG_00_v1_archived_contig_323 600 3 71M4S = 533 -138 TCGTACCAAGTTTTCACATCTTTTAACATAGATTCATTTTTAAAACTATATGTTTCAAGTACACATATAATCCCA B@C>CCCDDEEDEA@@CB?@CC=)A;HEG@;JIGHAGIGGHIIHFIGICGHAHEBIIIHFBFBFBD??*F?BFGG NH:i:2 HI:i:1 AS:i:144 nM:i:0 MD:Z:71
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