histograme python

from tkinter import *
from PIL import Image
from PIL import ImageTk
import tkinter
import tkinter.filedialog
import tkinter.colorchooser
import cv2
import matplotlib.pyplot as plt
import  os
import random
import numpy as np
import io
from tkinter.colorchooser import askcolor

def is_grey_scale(img_path):
    img = Image.open(img_path).convert('RGB')
    w,h = img.size
    for i in range(w):
        for j in range(h):
            r,g,b = img.getpixel((i,j))
            if r != g != b: return False
    return True

def selectareImagine():
    global labelA, labelB,labelC,labelD, changedPhoto, pozaInitiala, s, hist, x, valoriHist, poza, lungimePanou, inaltimePanou
    cale = tkinter.filedialog.askopenfilename()
    if not is_grey_scale(cale):
        if len(cale) > 0:
            poza = cv2.imread(cale)
            h, w, _ = poza.shape
            regularizator = 0
            if h > 2000 or w > 2000:
                regularizator = 10
            elif h > 400 and h < 2000 or w > 400 and w < 2000:
                regularizator = 5
            else: regularizator = 2

            lungimePanou = w//regularizator
            inaltimePanou = h//regularizator

            poza = cv2.resize(poza, (lungimePanou, inaltimePanou))
            poza = cv2.cvtColor(poza, cv2.COLOR_BGR2RGB)

            pozaInitiala = poza.copy()
            imageCopy = pozaInitiala.copy()


            changedPhoto = Image.fromarray(imageCopy)
            changedPhoto = changedPhoto.convert('L')
            changedPhotoArray = np.array(changedPhoto)


            hist = cv2.calcHist([changedPhotoArray],[0],None,[256],[0,256])
            x = list(range(0, 256))
            valoriHist = [item for sublist in hist for item in sublist]
            buf = io.BytesIO()
            plt.plot(hist)

            plt.grid(axis='y', alpha=1, linewidth=slider.get())
            plt.grid(axis='x', alpha=1, linewidth=slider.get())
            plt.savefig(buf, format='png')
            im = Image.open(buf)
            im = np.array(im)
            buf.close()
            plt.clf()
            color = ['b','g','r']
            buf = io.BytesIO()
            for i, col in enumerate(color):
                histColor = cv2.calcHist([pozaInitiala],[i],None,[256],[0,256])
                plt.plot(histColor, color = col)


            plt.grid(axis='y', alpha=1, linewidth=slider.get())
            plt.grid(axis='x', alpha=1, linewidth=slider.get())
            plt.savefig(buf, format='png')
            im_color = Image.open(buf)
            im_color = np.array(im_color)

            buf.close()
            plt.clf()


            poza = Image.fromarray(poza)
            poza = ImageTk.PhotoImage(poza)
            poza_hist = Image.fromarray(im)
            poza_hist = ImageTk.PhotoImage(poza_hist)
            poza_hist_color = Image.fromarray(im_color)
            poza_hist_color = ImageTk.PhotoImage(poza_hist_color)
            changedPhoto = ImageTk.PhotoImage(changedPhoto)



            if labelA is None or labelB is None:
                labelA = Label(leftframe,image = poza, width = lungimePanou,height = inaltimePanou)#1
                labelA.image = poza
                labelA.pack(side="top", padx=10, pady=10)
                labelC = Label(rightframe,image=poza_hist_color, width = lungimePanou,height = inaltimePanou)#4
                labelC.image = poza_hist_color
                labelC.pack(side="top", padx=10, pady=10)
                labelB = Label(leftframe,image=changedPhoto, width = lungimePanou,height = inaltimePanou)#2
                labelB.image = changedPhoto
                labelB.pack(side="bottom", padx=10, pady=10)
                labelD = Label(rightframe,image=poza_hist, width = lungimePanou,height = inaltimePanou)#3
                labelD.image = poza_hist
                labelD.pack(side="bottom", padx=10, pady=10)

            else:
                labelA.configure(image = poza)
                labelB.configure(image = changedPhoto)
                labelC.configure(image = poza_hist_color)
                labelD.configure(image = poza_hist)
                labelA.image = poza
                labelB.image = changedPhoto
                labelC.image = poza_hist_color
                labelD.image = poza_hist
    return

def thickness_grid():
    global labelA, labelB, labelC, labelD, color_code_x_axis, r, g , b, checkVar


    buf = io.BytesIO()

    plt.plot(hist)
    (r, g, b), _ = color_code_y_axis
    plt.grid(axis='y', alpha=checkVar.get(), linewidth=slider.get(),color=(r/255,g/255,b/255,1))
    (r, g, b), _ = color_code_x_axis
    plt.grid(axis='x', alpha=checkVar.get(), linewidth=slider.get(),color=(r/255,g/255,b/255,1))
    plt.savefig(buf, format='png')
    im = Image.open(buf)
    im = np.array(im)
    buf.close()
    plt.clf()

    color = ['b','g','r']
    buf = io.BytesIO()
    for i, col in enumerate(color):
        histColor = cv2.calcHist([pozaInitiala],[i],None,[256],[0,256])
        plt.plot(histColor, color = col)


    (r, g, b), _ = color_code_y_axis
    plt.grid(axis='y', alpha=checkVar.get(), linewidth=slider.get(),color=(r/255,g/255,b/255,1))
    (r, g, b), _ = color_code_x_axis
    plt.grid(axis='x', alpha=checkVar.get(), linewidth=slider.get(),color=(r/255,g/255,b/255,1))
    plt.savefig(buf, format='png')
    im_color = Image.open(buf)
    im_color = np.array(im_color)

    buf.close()
    plt.clf()

    poza_hist = Image.fromarray(im)
    poza_hist = ImageTk.PhotoImage(poza_hist)
    poza_hist_color = Image.fromarray(im_color)
    poza_hist_color = ImageTk.PhotoImage(poza_hist_color)


    if labelA is None or labelB is None:
        labelA = Label(leftframe,image = poza, width = lungimePanou,height = inaltimePanou)#1
        labelA.image = poza
        labelA.pack(side="top", padx=10, pady=10)
        labelC = Label(rightframe,image=poza_hist_color, width = lungimePanou,height = inaltimePanou)#4
        labelC.image = poza_hist_color
        labelC.pack(side="top", padx=10, pady=10)
        labelB = Label(leftframe,image=changedPhoto, width = lungimePanou,height = inaltimePanou)#2
        labelB.image = changedPhoto
        labelB.pack(side="bottom", padx=10, pady=10)
        labelD = Label(rightframe,image=poza_hist, width = lungimePanou,height = inaltimePanou)#3
        labelD.image = poza_hist
        labelD.pack(side="bottom", padx=10, pady=10)

    else:
        labelA.configure(image = poza)
        labelB.configure(image = changedPhoto)
        labelC.configure(image = poza_hist_color)
        labelD.configure(image = poza_hist)
        labelA.image = poza
        labelB.image = changedPhoto
        labelC.image = poza_hist_color
        labelD.image = poza_hist
    return

def choose_color_input():
    global firstColor, color_code_x_axis, r, g , b
    firstColor = []
    color_code_x_axis = askcolor(title ="Choose color")
    if color_code_x_axis != (None,None):
        if color_y_axis["state"] == "disabled":
            color_y_axis["state"] = "normal"

def choose_color_y_axis():
    global secondColor , color_code_y_axis, r, g , b
    secondColor = []
    color_code_y_axis = askcolor(title ="Choose color")
    if color_code_y_axis != (None,None):
        if process["state"] == "disabled":
            process["state"] = "normal"
        if grid["state"] == "disabled":
            grid["state"] = "normal"


def gridOnOff():
    thickness_grid()

root = Tk()
root.title('Change Color Bucket')
labelA = None
labelB = None
pozaInitiala = None

bottomframe = Frame(root)
bottomframe.pack (side = BOTTOM)

leftframe = Frame(root)
leftframe.pack( side = LEFT )
rightframe = Frame(root)
rightframe.pack( side = RIGHT)


btn = Button(bottomframe, text="Upload", command = selectareImagine,height = 2)
btn.pack(side="bottom", fill="both", expand="no", padx="20")

color_input = Button(bottomframe, text = "Color input", command = choose_color_input,height = 2, width = 10)
color_input.pack( side = "bottom", fill = "both",padx="20", pady="10")

color_y_axis = Button(bottomframe, text = "Color y axis", command = choose_color_y_axis,height = 2, width = 10)
color_y_axis.pack( side = "bottom", fill = "both",padx="20")
color_y_axis["state"] = "disabled"

process = Button(bottomframe, text = "Process", command = thickness_grid ,height = 2, width=10)
process.pack( side = "bottom", fill = "both",padx="20", pady="10" )
process["state"] = "disabled"

slider = Scale(bottomframe, from_=0, to=10, orient=HORIZONTAL)
slider.set(0)
slider.pack(side = "bottom", fill = "both",padx="30" )


checkVar = IntVar()
grid = Checkbutton(bottomframe, text="Grid on/off" ,height = 2, width = 10, variable = checkVar , command = gridOnOff , onvalue = 1, offvalue = 0)
grid.pack( side = "bottom", fill = "both", padx = "20")
grid["state"] = "disabled"
root.mainloop()