# correct reads
~/install/canu/canu-1.6/Linux-amd64/bin/canu overlapper=minimap
   genomeSize=100M minReadLength=100 minOverlapLength=30 -correct
   -p 4T1_BC06 -d 4T1_BC06
   -nanopore-raw workspace/pass/barcode06/fastq_runid_*.fastq
# align reads
~/install/minimap2/minimap2 -p 10
   -a ~/db/fasta/mmus/ucsc/mmus_ucsc_all_cdna.idx
   -x splice <(pv all/4T1_BC06/4T1_BC06.correctedReads.fasta.gz) |
  samtools sort > 4T1_BC06_all_vs_mmusAll.bam