Pop the seeds on a pan

Weigh and grind seeds without shell to a fine powder using coffee grinder or mortar and pestle.

[ Note: Baking this powder at 300 ºF for aprox. 2 hours has shown to vaporize the other alkaloids not targeted ]

1/3 of sodium (bi)carbonate or calcium (hidro)oxide

Add 97% alcohol in your tube and let it sit for an hour to allow reaction to complete. We are making a crude bufotenine freebase at this step, which contains bufotenine along with undesired, nausea producing compounds, as well as plant oils and fats

Bufotenine is very heat tolerant and the water could safely be boiled away if you are in a hurry.

If the right consistency of paste was achieved in the previous step, then the sodium carbonate will have formed its hydrated state and we will be left with a hard dry cake. An ammonia smell will be apparent, indicating that the freebasing reaction has occurred. If an excess of water was used then just leave the jar uncovered and let the water evaporate away. Heat and a fan will speed up this operation. Bufotenine is very heat tolerant and the water could safely be boiled away if you are in a hurry.

1. Grind up the hard cake then cover with anhydrous acetone. The bufotenine will dissolve in the acetone forming a yellow solution while leaving behind any unused sodium carbonate behind. Pour off the acetone solution into a collection jar and repeat the process by adding more acetone for a total of three extractions.
2. Filter the combined three acetone extractions, through a cotton ball loosely stuffed in the narrow part of a funnel, to obtain a clear yellow acetone solution of crude bufotenine freebase.
3. Add the contents of a 500 mg fumaric acid capsule directly to this solution and observe an instantaneous crashing out of bufotenine fumarate. This is a defatting step and effectively removes the plant oils and fats. Let this sit for a while to allow the reaction to complete. The bufotenine fumarate will stick to the sides and bottom of the jar and the waste acetone containing the unwanted oils can be poured off. Allow any remaining acetone to evaporate away until its odour is gone.
   [Note: the fumaric acid is added as a powder; as it dissolves in the acetone, it reacts with the freebase bufotenine forming bufotenine fumarate which precipitates out of solution, leaving room for more fumaric acid to dissolve. ]
4. Add enough water (about 50 ml) to dissolve the bufotenine fumarate then add about a gram of sodium carbonate powder directly to this solution. Like magic a caramel coloured lump of freebase bufotenine will form. This lump should be flattened out while adding an excess of water, to allow complete penetration by the sodium carbonate. Let this reaction continue for a while to completion, then pour off the water and dry out the caramel bufotenine.
   [ Note: the powdered sodium carbonate dissolves in the water rapidly, causing the resulting bufotenine freebase oils to gather together into a clump of soft resinous material as it is pushed away from the more dense basic solution.
5. Completely dissolve the impure bufotenine in 5 mL of acetone then add 20 ml of naphtha ( 1 part acetone : 4 parts naphtha ). This will crash out the unwanted nausea causing compounds leaving pure bufotenine in an almost clear solution. Allow the reaction sufficient time to complete.
   [Note: a 1:4 ratio of acetone:naphtha works to clean out the nausea causing compounds. In this method, all the impure bufo is completely dissolved in acetone before adding the naphtha. When the naphtha is added, then the impurities are precipitated.
   This is different from the previous methods where the cosolvents are premixed before selectively dissolving the bufo, and ensures that all the bufo is extracted from the leftovers. ]
6. Use the medicine dropper to siphon off the clear bufotenine solution to another jar, leaving behind the nausea nasties.
7. Evaporate the solvent to obtain light tan, reasonably pure bufotenine freebase.

Bufotenin boiling point 320 °C