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Jul 16th, 2018
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  1. [mem_sam_pe] paired reads have different names:
  2. "HISEQ:90:C3UNJACXX:1:1107:16388:61141",
  3. "HISEQ:90:C3UNJACXX:3:1303:2008:58095"
  4. [mem_sam_pe] [mem_sam_pe] paired reads have different names:
  5. "HWI-ST1437:64:C3UM1ACXX:4:1214:3833:62731",
  6. "HWI-ST1437:64:C3UM1ACXX:4:1202:18519:45906"
  7.  
  8. # align
  9. bwa mem -R <read_group1> <ref.fa> <file1_1.fq> <file1_2.fq> -o <file_aln.sam>
  10. # convert
  11. samtools view -Sb <file_aln.sam> > <file_aln.bam> # sort samtools sort <file_aln.bam> -o <file_alnSRT.bam>
  12. # select mapped
  13. samtools view -h -F 4 <file_alnSRT.bam> -o <file_alnMAP.bam>
  14. # convert to fastq
  15. samtools fastq -1 <filemap_1.fq.gz> -2 <filemap_1.fq.gz> <file_alnMAP.bam>
  16. # re-align
  17. bwa mem -R <read_group> <ref2.fa> <filemap_1.fq.gz> <filemap_1.fq.gz> | samtools sort -o <file_realign.bam>
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