cd /home/debian/ap

# Start a virtualenv
source venv-ap/bin/activate

# This needs to happen inside the virtualenv and takes ~20 seconds
crossbar start

# Outside the virtualenv, perhaps in a different command line window
python3 /home/debian/myscript.py

[Unit]
Description=Start CB
After=network.target

[Service]
Type=simple
User=debian
ExecStartPre=source /home/debian/ap/venv-ap/bin/activate
ExecStart=cd /home/debian/ap/ && crossbar start
Restart=always

[Install]
WantedBy=multi-user.target