[[File:Cholesteroad.PNG|thumb|right|250x375px|What riveting gameplay.]]
The gimmick of Cholesteroad is that you are challenged with defeating an endless swarm of Cholesterons, yellow enemies which basically act as punching bags for Mario and Luigi. Small ones are encountered at the beginning of your rally, then are temporarily replaced with larger ones as you approach a rank. The rank thresholds for each special attack are as follows;

{| style="text-align: center; width: 80%; margin: 0 auto; border-collapse: collapse;" border="1" cellpadding="1" cellspacing="1"
|-style="background: #ABC;"
!width="10%"|Item
!width="10%"|B Rank
!width="10%"|A Rank
|-
|[[Green Shell]]
|100 Points
|150 Points
|-
|[[Yoo Who Cannon]]
|80 Points
|120 Points
|-
|[[Super Bouncer]]
|20 Points
|30 Points
|-
|[[Spin Pipe]]
|20 Points
|30 Points
|-
|[[Magic Window]]
|70 Points
|120 Points
|}