Kumars Pandas task

import pandas as pd

source_path = "replace-example.csv" # change to your path

df = pd.read_csv(source_path, sep='\t')
subset = df[["Gene", "Annotation", "No. isolates"]]

df = df.where(~df.notna(), 1) # fills all not null with 1
df = df.fillna(0) # fills all null with 0

df[["Gene", "Annotation", "No. isolates"]] = subset
df