My roster that's far superior to Dave's

21 Characters
Final Boss is giant Gaben, Galactus style.
More of a general Steam fighter than an actual indie fighter

Gordon Freeman (HL2)
Scientist (Garry's Mod)
Heavy (TF2)
Bloodseeker (Dota 2)
Invoker (Dota 2)
Chell (Portal)
The Witch (L4D)
Quote (Cave Story)
Lemeza Kosugi (La Mulana)
Rico Rodriguez (Just Cause 2)
Meat Boy (Super Meat Boy)
The Clawed Girl (They Bleed Pixels)
Johnny Gat (Saints Row)
Commander Video (Bit Trip)
Recette Lemongrass (Recettear)
The Dovahkiin (Skyrim)
Shogun/Roman General (Total War)
Raz (Psychonauts)
The Castle Crashers (Castle Crashers)
Filia (Skullgirls)
J.C. Denton (Deus Ex)