C:UsersAngelDesktopunodos-uno

from PyQt5.QtWidgets import QMainWindow,QApplication
from PyQt5 import QtGui
import pathlib
import os
from PyQt5 import uic


class Main(QMainWindow):
    def __init__(self):
        QMainWindow.__init__(self)
        uic.loadUi("ui/Prueba.ui",self)

        absolute = os.path.realpath("Animacion1.gif")
        print(absolute)
        self.movie = QtGui.QMovie(absolute)
        self.label.setMovie(self.movie)
        self.movie.start()

app = QApplication([])
m = Main()
m.show()
app.exec_()