for y in [TRA,TRB]:
            CloneSizes = y['nSeqCDR3'].value_counts()
            freq = CloneSizes.value_counts() / total_TCR
            plt.plot(freq,colors2[j])
            if j % 2 == 0:
                legend_text.append('TRA'+ x + '_' + pt)
            else:
                legend_text.append('TRB'+ x + '_' + pt)
            j += 1