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asherrar

GA/VG Error Log

May 8th, 2023
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  1. ## Output from one array process
  2.  
  3. GraphAligner bioconda 1.0.17b-
  4. Load graph from # graph location
  5. Build minimizer seeder from the graph
  6. Minimizer seeds, length 15, window size 20, density 10
  7. Seed cluster size 1
  8. Extend up to 5 seed clusters
  9. Alignment bandwidth 10, tangle effort 1000
  10. Clip alignment ends with identity < 66%
  11. X-drop DP score cutoff 14705
  12. Backtrace from 10 highest scoring local maxima per cluster
  13. write alignments to # gam file
  14. Align
  15. Alignment finished
  16. Input reads: 580260 (3658115898bp)
  17. Seeds found: 9491908149
  18. Seeds extended: 2887363
  19. Reads with a seed: 577823 (3658105894bp)
  20. Reads with an alignment: 577575 (3302469894bp)
  21. Alignments: 9417997 (3704266867bp) (8245932 additional alignments discarded)
  22. End-to-end alignments: 116749 (567688549bp)
  23. Alignment broke with some reads. Look at stderr output.
  24. surjecting back to graph
  25.  
  26. ## Errors from one array process
  27.  
  28. GraphAligner bioconda 1.0.17b-
  29. src/GraphAlignerBitvectorCommon.h:1118: Assertion 'previous.node(neighbor).endSlice.scoreEnd >= scoreHere-(eq?0:1)' failed. Read: m140612_020550_42156_c100652082550000001823118110071460_s1_p0/127893/42129_54356. Seed: 0+,0,0,0
  30. src/GraphAlignerBitvectorCommon.h:1118: Assertion 'previous.node(neighbor).endSlice.scoreEnd >= scoreHere-(eq?0:1)' failed. Read: m140612_082500_42156_c100652082550000001823118110071461_s1_p0/17608/11349_18935. Seed: 0+,0,0,0
  31. src/GraphAlignerBitvectorCommon.h:1118: Assertion 'previous.node(neighbor).endSlice.scoreEnd >= scoreHere-(eq?0:1)' failed. Read: m140614_092436_42156_c100652052550000001823118110071490_s1_p0/83855/20478_34565. Seed: 0+,0,0,0
  32. src/GraphAlignerBitvectorCommon.h:1118: Assertion 'previous.node(neighbor).endSlice.scoreEnd >= scoreHere-(eq?0:1)' failed. Read: m141004_003611_42156_c100717212550000001823136902281550_s1_p0/120532/0_11601. Seed: 0+,0,0,0
  33. warning:[vg::get_sequence_dictionary] No reference-sense paths available in the graph; falling back to generic paths.
  34. warning[vg::Surjector]: Refusing to perform very large alignment against 102712 bp strand split subgraph for read m140612_020550_42156_c100652082550000001823118110071460_s1_p0/641/0_28728; suppressing further warnings.
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