BitmapSource img = BitmapFrame.Create(uri);

BitmapSource img = BitmapFrame.Create(uri,BitmapCreateOptions.None,BitmapCacheOption.OnLoad);

MemoryStream data = new MemoryStream(File.ReadAllBytes(file));
BitmapSource bitmap = BitmapFrame.Create(data);

Stream stream = File.OpenRead(filename);
Bitmap template = new Bitmap(stream); // or (Bitmap) Bitmap.FromStream(stream)
stream.Close();