Joltzdude139's 1 Life Rules:

1) If I die, the run is over. (ex. bleeding out, falling off cliff, etc)
2) Things other than the player can die. (ex. FL4K's pets, Zane's clone, etc)
3) Fight for your life is okay, but you cannot save/quit until you get a 2nd wind.
4) No traveling or save/quitting in the middle of danger. If you can wait 5 seconds without taking damage, you're good.
5) Guardian rank must be disabled. Once you reach mayhem mode, you can turn it on.
6) No glitches.
7) No using mailed items sent by friends.
8) No golden chest.
9) Read only or dashboard farming is allowed.