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- Go to http://genome-test.soe.ucsc.edu/cgi-bin/hgTables to get Tilapia genome table
- Clade>Vertebrate Genome>Nile Tilapia Assembly>Nov. 2016(ASM185804v2/oreNil3)
- group>Mapping and Sequencing track>Assemple
- And get the 'output' file like this:
- #bin chrom chromStart chromEnd ix type frag fragStart fragEnd strand
- 585 chrM 0 16627 1 O NC_013663.1 0 16627 +
- 1 chrLG1 0 38372991 1 O MKQE01000001.1 0 38372991 +
- 1 chrLG2 0 35256741 1 O MKQE01000012.1 0 35256741 +
- 1 chrLG4 0 38038224 1 O MKQE01000018.1 0 38038224 +
- 1 chrLG5 0 34628617 1 O MKQE01000019.1 0 34628617 +
- 1 chrLG6 0 44571662 1 O MKQE01000020.1 0 44571662 +
- 1 chrLG7 0 62059223 1 O MKQE01000021.1 0 62059223 +
- 1 chrLG8 0 30802437 1 O MKQE01000022.1 0 30802437 +
- 1 chrLG9 0 27519051 1 O MKQE01000023.1 0 27519051 +
- 1 chrLG10 0 32426571 1 O MKQE01000002.1 0 32426571 +
- 1 chrLG11 0 36466354 1 O MKQE01000003.1 0 36466354 +
- 1 chrLG12 0 41232431 1 O MKQE01000004.1 0 41232431 +
- 1 chrLG13 0 32337344 1 O MKQE01000005.1 0 32337344 +
- 1 chrLG14 0 39264731 1 O MKQE01000006.1 0 39264731 +
- 1 chrLG15 0 36154882 1 O MKQE01000007.1 0 36154882 +
- 1 chrLG16 0 43860769 1 O MKQE01000008.1 0 43860769 +
- 1 chrLG17 0 40919683 1 O MKQE01000009.1 0 40919683 +
- 1 chrLG18 0 37007722 1 O MKQE01000010.1 0 37007722 +
- 1 chrLG19 0 31245232 1 O MKQE01000011.1 0 31245232 +
- 1 chrLG20 0 36767035 1 O MKQE01000013.1 0 36767035 +
- 1 chrLG22 0 37011614 1 O MKQE01000014.1 0 37011614 +
- 1 chrLG23 0 44097196 1 O MKQE01000015.1 0 44097196 +
- 1 chrLG3a 0 14041792 1 O MKQE01000016.1 0 14041792 +
- 1 chrLG3b 0 54508961 1 O MKQE01000017.1 0 54508961 +
- Then you have to turn this genome table file into this format:(or just use my format)
- chr - M M 0 16627 chrM
- chr - LG1 1 0 38372991 chr1
- chr - LG2 2 0 35256741 chr2
- chr - LG3a 3a 0 14041792 chr3
- chr - LG3b 3b 0 54508961 lum70chr3
- chr - LG4 4 0 38038224 chr4
- chr - LG5 5 0 34628617 chr5
- chr - LG6 6 0 44571662 chr6
- chr - LG7 7 0 62059223 chr7
- chr - LG8 8 0 30802437 chr8
- chr - LG9 9 0 27519051 chr9
- chr - LG10 10 0 32426571 chr10
- chr - LG11 11 0 36466354 chr11
- chr - LG12 12 0 41232431 chr12
- chr - LG13 13 0 32337344 chr13
- chr - LG14 14 0 39264731 chr14
- chr - LG15 15 0 36154882 chr15
- chr - LG16 16 0 43860769 chr16
- chr - LG17 17 0 40919683 chr17
- chr - LG18 18 0 37007722 chr18
- chr - LG19 19 0 31245232 chr19
- chr - LG20 20 0 36767035 chr20
- chr - LG22 22 0 37011614 chr22
- chr - LG23 23 0 44097196 chr23
- Save these text as karyotype.nile.tilapia.oreNil3.txt
- put it into /$anaconda_install_path/data/karyotype/
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