dict_items = dict()

with open('config.txt', 'r') as r:
    list_strings = r.read().splitlines()

for string in list_strings:
    splited = string.split(' - ')
    splited = [i.replace('"', '') for i in splited]
    if len(splited) == 2:
        dict_items[splited[0]] = splited[1]

print(dict_items)
# {'One': '1024', 'Two': '1234'}