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  1. - Function of DNA
  2. ○ The information in a segment of DNA lies in the sequence of bases that exists along it length
  3. ○ A section of DNA that codes for one particular protein is called a gene
  4. - Protein Manufacture
  5. Codon - D Small structure in the cytoplasm where mRNA is translated into an amino acid chain. - 2
  6.    
  7. Ribosome - A A base only found in RNA, which replaces the base thymine found in DNA. - 4
  8.    
  9. Polypeptide - E A strand of mRNA is produced by complimentary pairing of bases with one strand of DNA in the nucleus - 5
  10.  
  11. Uracil - B Alternative name for a base pair triplet. - 1
  12.    
  13. Transcription - C A chain of amino acids that will form part of a protein.
  14.    - 3
  15.  
  16. - Function of protein
  17. ○ Proteins determine physical and metabolic traits and regulate growth and development
  18. § Structural proteins - e.g. dystrophin (in muscle), collagen (in connective tissue)
  19. § Enzymes - e.g. amylase, catalase
  20. § Hormones - insulin, glucagon, growth hormone
  21. § Transport molecules - haemoglobin
  22. - Research task
  23. ○ Find a protein that interests you
  24. ○ I.E - Proteins to do with
  25. § Muscle recovery
  26. § Cosmetics (skin proteins)
  27. § Beauty (Hair)
  28. ○ Create a quick summary
  29. ○ Keratin
  30. § Keratin is a major protein in skin, hair and nails. Keratin acts as an external protective protein in the outer layer of a hair strand and it's used as a structural protein in the inner core of hair which helps strengthen the hair
  31. - Proteins
  32. ○ Proteins are also polymers
  33. § They are made of amino acids (Monomers)
  34. § A long chain of amino acids make up a protein
  35. § There are 20 different amino acids
  36. - Codons or triplets
  37. ○ A codon is a group of three nucleotides
  38. ○ Codons code for specific amino acids
  39. - Why only 20 amino acids
  40. ○ Some amino acids are coded for by multiple codons
  41. § Serine is coded for by 6 different codons
  42. ○ Decreases the chance of a 'mutation' having an effect
  43. - Essential Amino Acids
  44. ○ Some amino acids can't be manufactured by our bodies fast enough to meet demand
  45. ○ We must acquire these amino acids through our diet
  46. § Histidine
  47. § Phenylalanine
  48. § Isoleucine
  49. § Lysine
  50. § Methionine
  51. § Leucine
  52. § Threonine
  53. § Tryptophan
  54. § Valine
  55. - RNA - Ribonucleic acid
  56. ○ Contains the sugar ribosome rather than deoxyribose
  57. ○ Found in the nucleus as well as in the cytosol and ribosomes
  58. ○ Three forms: mRNA, tRNA and rRNA
  59. ○ Single stranded
  60. ○ Contains Uracil instead of Thymine
  61. ○ RNA is SINGLE stranded
  62. - Protein Synthesis
  63. ○ RNA is a link between DNA and proteins
  64. § Transcription
  65. § Translations
  66. □ Instructions for code are sent from nucleus, are made in the ribosomes and then the proteins are made then sent to the Golgi to be packaged and processed
  67. □ Transcription makes three types of RNA
  68. □ Transcription copies DNA to make a strand of RNA
  69. § -DNA --- RNA
  70. - Transcription Overview
  71. ○ Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make RNA molecule
  72. ○ Transcription is performed by enzymes called RNA Polymerases, which link nucleotides to form an RNA strand (using a DNA strand as a template)
  73. ○ Transcription has three stages
  74. § Initiation (START)
  75. § Elongation (EXTEND)
  76. § And termination (STOP)
  77. § Think of these as traffic lights - Initiation is green and Termination is red
  78. - Transcription
  79. ○ Takes place in the nucleus
  80. § 1 - DNA unzips (double stranded DNA separate) - one strand is used as a template (Helicase Enzyme)
  81. § 2 - Complementary bases attach to the strand being copied - C joins to G and so on
  82. § 3 - Thymine (Base T) is not present and a different base, U, joins with A in the way that T would've done
  83. § 4 - This forms a strand of messenger RNA (mRNA)
  84. § 5 - Messenger RNA is small enough to move out of nucleus and so it travels to the ribosomes in the cytoplasm
  85. ○ Main enzymes involved in transcription in RNA polymerase
  86. ○ Uses a single-stranded DNA template to make RNA
  87. ○ RNA polymerase builds an RNA strand in the 5' to 3' direction (Adding to the 3' end)
  88. ○ RNA polymerase "walks" along one strand of DNA
  89. ○ RNA adds a matching nucleotide to the 3' end of the RNA strand
  90. ○ Three types of RNA
  91. § Messenger RNA (mRNA) carries the message that will be translated to form a protein
  92. § Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made
  93. § Transfer RNA (tRNA) translates amino acids from the cytoplasm
  94. - Coding
  95. ○ AGTACGTCA
  96. ○ UCAUGCAGU
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