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- - Function of DNA
- ○ The information in a segment of DNA lies in the sequence of bases that exists along it length
- ○ A section of DNA that codes for one particular protein is called a gene
- - Protein Manufacture
- Codon - D Small structure in the cytoplasm where mRNA is translated into an amino acid chain. - 2
- Ribosome - A A base only found in RNA, which replaces the base thymine found in DNA. - 4
- Polypeptide - E A strand of mRNA is produced by complimentary pairing of bases with one strand of DNA in the nucleus - 5
- Uracil - B Alternative name for a base pair triplet. - 1
- Transcription - C A chain of amino acids that will form part of a protein.
- - 3
- - Function of protein
- ○ Proteins determine physical and metabolic traits and regulate growth and development
- § Structural proteins - e.g. dystrophin (in muscle), collagen (in connective tissue)
- § Enzymes - e.g. amylase, catalase
- § Hormones - insulin, glucagon, growth hormone
- § Transport molecules - haemoglobin
- - Research task
- ○ Find a protein that interests you
- ○ I.E - Proteins to do with
- § Muscle recovery
- § Cosmetics (skin proteins)
- § Beauty (Hair)
- ○ Create a quick summary
- ○ Keratin
- § Keratin is a major protein in skin, hair and nails. Keratin acts as an external protective protein in the outer layer of a hair strand and it's used as a structural protein in the inner core of hair which helps strengthen the hair
- - Proteins
- ○ Proteins are also polymers
- § They are made of amino acids (Monomers)
- § A long chain of amino acids make up a protein
- § There are 20 different amino acids
- - Codons or triplets
- ○ A codon is a group of three nucleotides
- ○ Codons code for specific amino acids
- - Why only 20 amino acids
- ○ Some amino acids are coded for by multiple codons
- § Serine is coded for by 6 different codons
- ○ Decreases the chance of a 'mutation' having an effect
- - Essential Amino Acids
- ○ Some amino acids can't be manufactured by our bodies fast enough to meet demand
- ○ We must acquire these amino acids through our diet
- § Histidine
- § Phenylalanine
- § Isoleucine
- § Lysine
- § Methionine
- § Leucine
- § Threonine
- § Tryptophan
- § Valine
- - RNA - Ribonucleic acid
- ○ Contains the sugar ribosome rather than deoxyribose
- ○ Found in the nucleus as well as in the cytosol and ribosomes
- ○ Three forms: mRNA, tRNA and rRNA
- ○ Single stranded
- ○ Contains Uracil instead of Thymine
- ○ RNA is SINGLE stranded
- - Protein Synthesis
- ○ RNA is a link between DNA and proteins
- § Transcription
- § Translations
- □ Instructions for code are sent from nucleus, are made in the ribosomes and then the proteins are made then sent to the Golgi to be packaged and processed
- □ Transcription makes three types of RNA
- □ Transcription copies DNA to make a strand of RNA
- § -DNA --- RNA
- - Transcription Overview
- ○ Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make RNA molecule
- ○ Transcription is performed by enzymes called RNA Polymerases, which link nucleotides to form an RNA strand (using a DNA strand as a template)
- ○ Transcription has three stages
- § Initiation (START)
- § Elongation (EXTEND)
- § And termination (STOP)
- § Think of these as traffic lights - Initiation is green and Termination is red
- - Transcription
- ○ Takes place in the nucleus
- § 1 - DNA unzips (double stranded DNA separate) - one strand is used as a template (Helicase Enzyme)
- § 2 - Complementary bases attach to the strand being copied - C joins to G and so on
- § 3 - Thymine (Base T) is not present and a different base, U, joins with A in the way that T would've done
- § 4 - This forms a strand of messenger RNA (mRNA)
- § 5 - Messenger RNA is small enough to move out of nucleus and so it travels to the ribosomes in the cytoplasm
- ○ Main enzymes involved in transcription in RNA polymerase
- ○ Uses a single-stranded DNA template to make RNA
- ○ RNA polymerase builds an RNA strand in the 5' to 3' direction (Adding to the 3' end)
- ○ RNA polymerase "walks" along one strand of DNA
- ○ RNA adds a matching nucleotide to the 3' end of the RNA strand
- ○ Three types of RNA
- § Messenger RNA (mRNA) carries the message that will be translated to form a protein
- § Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made
- § Transfer RNA (tRNA) translates amino acids from the cytoplasm
- - Coding
- ○ AGTACGTCA
- ○ UCAUGCAGU
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