from threading import Thread
from Tkinter import *
from time import sleep


class _timeout(Thread):
    def __init__(self, finestra):
        Thread.__init__(self)
        self.finestra = finestra

    def run(self):
        sleep(5)
        print('asd')
        self.finestra.destroy()


class AppBase:
    def __init__(self, titolo):
        self.finestra = Tk()
        self.finestra.title(titolo)
        quadro1 = Frame(self.finestra)
        my_thread = _timeout(self.finestra)
        my_thread.start()
        self.finestra.mainloop()

AppBase('test multi')