estwin = [list1, list2, list3, list4]
output=([])
    estwin.reverse()
    for i in range(len(estwin) -1):
        difference = Diff(estwin[i], estwin[i+1])
        output.append(difference)
    return(output)

def Diff(li_bigger, li_smaller):
    c = [x for x in li_bigger if x not in li_smaller]
    return (c)