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- > library("ChAMP")
- Loading required package: minfi
- Loading required package: BiocGenerics
- Loading required package: parallel
- Attaching package: ‘BiocGenerics’
- The following objects are masked from ‘package:parallel’:
- clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport, clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply, parSapply, parSapplyLB
- The following objects are masked from ‘package:stats’:
- IQR, mad, sd, var, xtabs
- The following objects are masked from ‘package:base’:
- anyDuplicated, append, as.data.frame, cbind, colMeans, colnames, colSums, do.call, duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect, is.unsorted, lapply,
- lengths, Map, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind, Reduce, rowMeans, rownames, rowSums, sapply, setdiff, sort, table,
- tapply, union, unique, unsplit, which, which.max, which.min
- Loading required package: GenomicRanges
- Loading required package: stats4
- Loading required package: S4Vectors
- Attaching package: ‘S4Vectors’
- The following object is masked from ‘package:base’:
- expand.grid
- Loading required package: IRanges
- Loading required package: GenomeInfoDb
- Loading required package: SummarizedExperiment
- Loading required package: Biobase
- Welcome to Bioconductor
- Vignettes contain introductory material; view with 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")', and for packages 'citation("pkgname")'.
- Loading required package: DelayedArray
- Loading required package: matrixStats
- Attaching package: ‘matrixStats’
- The following objects are masked from ‘package:Biobase’:
- anyMissing, rowMedians
- Attaching package: ‘DelayedArray’
- The following objects are masked from ‘package:matrixStats’:
- colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges
- The following object is masked from ‘package:base’:
- apply
- Loading required package: Biostrings
- Loading required package: XVector
- Attaching package: ‘Biostrings’
- The following object is masked from ‘package:DelayedArray’:
- type
- The following object is masked from ‘package:base’:
- strsplit
- Loading required package: bumphunter
- Loading required package: foreach
- foreach: simple, scalable parallel programming from Revolution Analytics
- Use Revolution R for scalability, fault tolerance and more.
- http://www.revolutionanalytics.com
- Loading required package: iterators
- Loading required package: locfit
- locfit 1.5-9.1 2013-03-22
- Setting options('download.file.method.GEOquery'='auto')
- Setting options('GEOquery.inmemory.gpl'=FALSE)
- Loading required package: ChAMPdata
- Loading required package: FEM
- Loading required package: AnnotationDbi
- Loading required package: Matrix
- Attaching package: ‘Matrix’
- The following object is masked from ‘package:S4Vectors’:
- expand
- Loading required package: marray
- Loading required package: limma
- Attaching package: ‘limma’
- The following object is masked from ‘package:BiocGenerics’:
- plotMA
- Loading required package: corrplot
- Loading required package: igraph
- Attaching package: ‘igraph’
- The following object is masked from ‘package:Biostrings’:
- union
- The following object is masked from ‘package:GenomicRanges’:
- union
- The following object is masked from ‘package:IRanges’:
- union
- The following object is masked from ‘package:S4Vectors’:
- union
- The following objects are masked from ‘package:BiocGenerics’:
- normalize, union
- The following objects are masked from ‘package:stats’:
- decompose, spectrum
- The following object is masked from ‘package:base’:
- union
- Loading required package: impute
- Loading required package: org.Hs.eg.db
- Loading required package: graph
- Attaching package: ‘graph’
- The following objects are masked from ‘package:igraph’:
- degree, edges, intersection
- The following object is masked from ‘package:Biostrings’:
- complement
- Loading required package: DMRcate
- Loading required package: DSS
- Loading required package: bsseq
- Attaching package: ‘bsseq’
- The following object is masked from ‘package:minfi’:
- getMeth
- Loading required package: splines
- Loading required package: DMRcatedata
- Loading required package: Illumina450ProbeVariants.db
- Loading required package: IlluminaHumanMethylationEPICmanifest
- Package loaded
- ___ _ _ __ __ ___
- / __| |_ /_\ | \/ | _ \
- | (__| ' \ / _ \| |\/| | _/
- \___|_||_/_/ \_\_| |_|_|
- ------------------------------
- ChAMP provides comprehensive integrated analysis pipeline for DNA methylation HumanMethylation Beadchip.
- You may use vignette("ChAMP") to view html version guildbook.
- If you have any question or suggestion about ChAMP, please email to [email protected].
- There were 15 warnings (use warnings() to see them)
- > testDir=system.file("extdata",package="ChAMPdata")
- > myLoad <- champ.load(testDir,arraytype="450K")
- [===========================]
- [<<<< ChAMP.LOAD START >>>>>]
- -----------------------------
- Loading data from C:/Program Files/R/R-3.4.0patched/library/ChAMPdata/extdata
- [read.metharray.sheet] Found the following CSV files:
- [1] "C:/Program Files/R/R-3.4.0patched/library/ChAMPdata/extdata/lung_test_set.csv"
- Loading required package: IlluminaHumanMethylation450kmanifest
- << Read DataSet Success. >>
- The fraction of failed positions per sample
- (You may need to delete samples with high proportion of failed probes
- ):
- Failed CpG Fraction.
- C1 0.0013429122
- C2 0.0022162171
- C3 0.0003563249
- C4 0.0002842360
- T1 0.0003831007
- T2 0.0011946152
- T3 0.0014953286
- T4 0.0015447610
- Filtering probes with a detection p-value above 0.01 in one or more samples has removed 2728 probes from the analysis. If a large number of probes have been removed, ChAMP suggests you to identify potentially bad samples.
- << Filter DetP Done. >>
- Error in (function (classes, fdef, mtable) :
- unable to find an inherited method for function 'assayData' for signature '"RGChannelSetExtended"'
- > sessionInfo()
- R version 3.4.0 Patched (2017-05-05 r72662)
- Platform: x86_64-w64-mingw32/x64 (64-bit)
- Running under: Windows >= 8 x64 (build 9200)
- Matrix products: default
- locale:
- [1] LC_COLLATE=English_United Kingdom.1252 LC_CTYPE=English_United Kingdom.1252 LC_MONETARY=English_United Kingdom.1252 LC_NUMERIC=C
- [5] LC_TIME=English_United Kingdom.1252
- attached base packages:
- [1] splines stats4 parallel stats graphics grDevices utils datasets methods base
- other attached packages:
- [1] IlluminaHumanMethylation450kmanifest_0.4.0 ChAMP_2.9.0 IlluminaHumanMethylationEPICmanifest_0.3.0 Illumina450ProbeVariants.db_1.13.0
- [5] DMRcate_1.13.0 DMRcatedata_1.13.0 DSS_2.17.0 bsseq_1.13.2
- [9] FEM_3.5.0 graph_1.55.0 org.Hs.eg.db_3.4.1 impute_1.51.0
- [13] igraph_1.0.1 corrplot_0.77 marray_1.55.0 limma_3.33.2
- [17] Matrix_1.2-10 AnnotationDbi_1.39.0 ChAMPdata_2.9.0 minfi_1.23.2
- [21] bumphunter_1.17.0 locfit_1.5-9.1 iterators_1.0.8 foreach_1.4.3
- [25] Biostrings_2.45.0 XVector_0.17.0 SummarizedExperiment_1.7.2 DelayedArray_0.3.4
- [29] matrixStats_0.52.2 Biobase_2.37.2 GenomicRanges_1.29.3 GenomeInfoDb_1.13.1
- [33] IRanges_2.11.1 S4Vectors_0.15.1 BiocGenerics_0.23.0 BiocInstaller_1.27.2
- loaded via a namespace (and not attached):
- [1] R.utils_2.5.0 IlluminaHumanMethylationEPICanno.ilm10b2.hg19_0.6.0 RSQLite_1.1-2
- [4] htmlwidgets_0.8 trimcluster_0.1-2 grid_3.4.0
- [7] BiocParallel_1.11.1 munsell_0.4.3 codetools_0.2-15
- [10] preprocessCore_1.39.0 statmod_1.4.29 colorspace_1.3-2
- [13] fastICA_1.2-0 knitr_1.15.1 robustbase_0.92-7
- [16] JADE_2.0-0 isva_1.9 GenomeInfoDbData_0.99.0
- [19] biovizBase_1.25.1 diptest_0.75-7 R6_2.2.0
- [22] doParallel_1.0.10 illuminaio_0.19.0 clue_0.3-53
- [25] AnnotationFilter_1.1.0 flexmix_2.3-14 bitops_1.0-6
- [28] reshape_0.8.6 assertthat_0.2.0 IlluminaHumanMethylation450kanno.ilmn12.hg19_0.6.0
- [31] scales_0.4.1 nnet_7.3-12 gtable_0.2.0
- [34] methylumi_2.23.0 sva_3.25.0 ensembldb_2.1.3
- [37] genefilter_1.59.0 rtracklayer_1.37.0 lazyeval_0.2.0
- [40] acepack_1.4.1 GEOquery_2.43.0 dichromat_2.0-0
- [43] checkmate_1.8.2 yaml_2.1.14 reshape2_1.4.2
- [46] GenomicFeatures_1.29.1 backports_1.0.5 httpuv_1.3.3
- [49] qvalue_2.9.0 Hmisc_4.0-3 tools_3.4.0
- [52] nor1mix_1.2-2 ggplot2_2.2.1 RColorBrewer_1.1-2
- [55] DNAcopy_1.51.0 siggenes_1.51.0 Rcpp_0.12.10
- [58] plyr_1.8.4 base64enc_0.1-3 progress_1.1.2
- [61] zlibbioc_1.23.0 purrr_0.2.2 RCurl_1.95-4.8
- [64] BiasedUrn_1.07 prettyunits_1.0.2 rpart_4.1-11
- [67] openssl_0.9.6 viridis_0.4.0 cluster_2.0.6
- [70] magrittr_1.5 data.table_1.10.4 goseq_1.29.0
- [73] mvtnorm_1.0-6 wateRmelon_1.21.0 whisker_0.3-2
- [76] ProtGenerics_1.9.0 missMethyl_1.11.0 RPMM_1.25
- [79] mime_0.5 xtable_1.8-2 XML_3.98-1.7
- [82] mclust_5.2.3 gridExtra_2.2.1 compiler_3.4.0
- [85] biomaRt_2.33.1 tibble_1.3.0 R.oo_1.21.0
- [88] htmltools_0.3.6 mgcv_1.8-17 Formula_1.2-1
- [91] tidyr_0.6.2 DBI_0.6-1 geneLenDataBase_1.13.0
- [94] MASS_7.3-47 fpc_2.1-10 permute_0.9-4
- [97] quadprog_1.5-5 R.methodsS3_1.7.1 Gviz_1.21.1
- [100] RefFreeEWAS_2.1 GenomicAlignments_1.13.1 registry_0.3
- [103] foreign_0.8-68 plotly_4.6.0 annotate_1.55.0
- [106] rngtools_1.2.4 pkgmaker_0.22 multtest_2.33.0
- [109] beanplot_1.2 ruv_0.9.6 doRNG_1.6.6
- [112] stringr_1.2.0 VariantAnnotation_1.23.0 digest_0.6.12
- [115] base64_2.0 htmlTable_1.9 dendextend_1.5.2
- [118] kernlab_0.9-25 curl_2.6 shiny_1.0.3
- [121] Rsamtools_1.29.0 gtools_3.5.0 modeltools_0.2-21
- [124] nlme_3.1-131 jsonlite_1.4 viridisLite_0.2.0
- [127] BSgenome_1.45.1 lattice_0.20-35 DEoptimR_1.0-8
- [130] httr_1.2.1 survival_2.41-3 GO.db_3.4.1
- [133] interactiveDisplayBase_1.15.0 shinythemes_1.1.1 prabclus_2.2-6
- [136] class_7.3-14 stringi_1.1.5 AnnotationHub_2.9.1
- [139] latticeExtra_0.6-28 memoise_1.1.0 dplyr_0.5.0
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