fasta alignment

#!c:/Python26/python.exe

import cgi
import os
import cgitb; cgitb.enable()
print 'Content-type: text/html\n\n'
from index import HtmlTemplate

t = HtmlTemplate()
t.Header()
t.Body()


form=cgi.FieldStorage()
a=(form["dfile"].filename)
(name,ext) = os.path.splitext(a)
import sys, subprocess
from Bio import AlignIO
from Bio import SeqIO
from Bio.Align.Applications import ClustalwCommandline
cline = ClustalwCommandline("clustalw", infile=a, seqnos="ON")
child = subprocess.call(str(cline), shell=(sys.platform!="win32"))
alignfile=name+'.aln'
alignment =AlignIO.read(alignfile, "clustal")
print "<b><u> Result Analysis</u></b></br>"
print "Input file name : "+a
print "<br>"
print "Total Number of Records: %i" % len(alignment)
handle=open(alignfile,'rU')
for seql in SeqIO.parse(handle, "clustal"):
    seq_length = len(seql.seq)
print "<br>"
print "Total number of Sequences: %i" % seq_length
print "<br>"


print "<b><u> Alignment Result</u></b></br>"
print "<pre>"
f = open(alignfile)
for line in f.readlines():
    print line
print "</pre>"
print "</br>"

print "<b><u> Guide Tree Result</u></b></br>"
print "<pre>"
f = open(name + '.dnd')
for line in f.readlines():
    print line
print "</pre>"

t.Footer()