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- PRELAB QUESTIONS:
- 1. Explain the difference in structure and function between a protein, a lipid and a nucleic acid.
- 2. Explain the process of DNA extraction. What occurs at each step of the process?
- 3. Explain the effect soaps have on lipids.
- 4. What is a co-factor?
- 5. What is a buffer? How does it work?
- 6. Define the following terms
- 1. Restriction fragment length polymorphism
- 2. restriction enzyme
- 3. ligase .
- 4. PCR
- 5. human genome
- 7. What must be used to create the DNA fragments for the electrophoresis? Explain how these enzymes function. How are these fragments separated?
- 8. Explain the process of restriction fragment analysis. How accurate is it and why?
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