import win32gui

def get_pixel_colour(i_x, i_y):

    i_desktop_window_id = win32gui.GetDesktopWindow()
    i_desktop_window_dc = win32gui.GetWindowDC(i_desktop_window_id)
    long_colour = win32gui.GetPixel(i_desktop_window_dc, i_x, i_y)
    i_colour = int(long_colour)
    return (i_colour & 0xff), ((i_colour >> 8) & 0xff), ((i_colour >> 16) & 0xff)

print (get_pixel_colour(0, 0))