from tkinter import *

def do():
    global top
    top = Toplevel(root)
    button=Button(top, text = "Click", command = pis).pack()
def pis():
    global top
    label = Label(top, text="You clicked").pack()
root = Tk()


button=Button(root, text = "Click", command = do).pack()

root.mainloop()

import os
os.environ['TCL_LIBRARY'] = "C:\Users\<username>\AppData\Local\Programs\Python\Python35-32\tcl\tcl8.6"
os.environ['TK_LIBRARY'] = "C:\Users\<username>\AppData\Local\Programs\Python\Python35-32\tcl\tk8.6"

base = None

if sys.platform == 'win32':
    base = "Win32GUI"

executables = [cx_Freeze.Executable("prog.py", base=base)]

cx_Freeze.setup(
    name = "SeaofBTC-Client",
    options = {"build_exe": {"packages":["tkinter"], "include_files":[]}},
    version = "0.01",
    description = "Sea of BTC trading application",
    executables = executables
    )