<script type="text/javascript" src="../Scripts/jquery-1.2.6.js"></script>

<script type="text/javascript" src="/Scripts/jquery-1.2.6.js"></script>

<img src="~/Content/MyImage.jpg">

<script type="text/javascript" src="<%=Url.Content("~/Scripts/jquery-1.2.6.js")%>"></script>

<%=Html.ScriptInclude("~/Content/Script/jquery.1.2.6.js")%>

<a href="@Url.Content("~/Home")">Application home page</a>

<a href="~/Home">Application home page</a>

<script src="<%=ResolveUrl("~/Scripts/jquery-1.2.6.min.js") %>" type="text/javascript"></script>

using System;
using System.IO;
using System.Text;
using System.Text.RegularExpressions;
using System.Web;

namespace Demo
{
    public class PathRewriter : Stream
    {
        Stream filter;
        HttpContext context;
        object writeLock = new object();
        StringBuilder sb = new StringBuilder();

        Regex eofTag = new Regex("</html>", RegexOptions.IgnoreCase | RegexOptions.Compiled);
        Regex rootTag = new Regex("/_AppRoot_", RegexOptions.IgnoreCase | RegexOptions.Compiled);

        public PathRewriter(Stream filter, HttpContext context)
        {
            this.filter = filter;
            this.context = context;
        }

        public override void Write(byte[] buffer, int offset, int count)
        {
            string temp;

            lock (writeLock)
            {
                temp = Encoding.UTF8.GetString(buffer, offset, count);
                sb.Append(temp);

                if (eofTag.IsMatch(temp))
                    RewritePaths();
            }
        }

        public void RewritePaths()
        {
            byte[] buffer;
            string temp;
            string root;

            temp = sb.ToString();
            root = context.Request.ApplicationPath;
            if (root == "/") root = "";

            temp = rootTag.Replace(temp, root);
            buffer = Encoding.UTF8.GetBytes(temp);
            filter.Write(buffer, 0, buffer.Length);
        }

        public override bool CanRead
        {
            get { return true; }
        }

        public override bool CanSeek
        {
            get { return filter.CanSeek; }
        }

        public override bool CanWrite
        {
            get { return true; }
        }

        public override void Flush()
        {
            return;
        }

        public override long Length
        {
            get { return Encoding.UTF8.GetBytes(sb.ToString()).Length; }
        }

        public override long Position
        {
            get { return filter.Position; }
            set { filter.Position = value; }
        }

        public override int Read(byte[] buffer, int offset, int count)
        {
            return filter.Read(buffer, offset, count);
        }

        public override long Seek(long offset, SeekOrigin origin)
        {
            return filter.Seek(offset, origin);
        }

        public override void SetLength(long value)
        {
            throw new NotImplementedException();
        }
    }

    public class PathFilterModule : IHttpModule
    {
        public void Dispose()
        {
            return;
        }

        public void Init(HttpApplication context)
        {
            context.ReleaseRequestState += new EventHandler(context_ReleaseRequestState);
        }

        void context_ReleaseRequestState(object sender, EventArgs e)
        {
            HttpApplication app = sender as HttpApplication;
            if (app.Response.ContentType == "text/html")
                app.Response.Filter = new PathRewriter(app.Response.Filter, app.Context);
        }
    }
}

<a href="@Url.Content("~/Home")">Application home page</a>

protected void Application_BeginRequest(object sender, EventArgs e)
 {
     Request.ServerVariables.Remove("IIS_WasUrlRewritten");
 }

<script type="text/javascript" src="/MyProject/Scripts/jquery-1.2.6.js"></script>

<a href=@Helper.Root()/about">About Us</a>

public static string Root()
{
    if (HttpContext.Current.Request.Url.Host == "localhost")
    {
        return "";
    }
    else
    {
        return "/productionroot";
    }
}