Python add record to MariaDB

#!/usr/bin/env python
# -*- coding: utf-8 -*-
import pymysql
# Open file with elail addresses : separate email @dress per line
file = open('my_text_file.txt','r')
fo = file.readlines()
# Connect to the database
connection = pymysql.connect(host='mariadb55.websupport.sk',
                 port=3310,
                             user='my_sql_user',
                             passwd='my_sql_pass',
                             db='my_sql_db',
                             charset='utf8mb4',
                             cursorclass=pymysql.cursors.DictCursor)
# Read email from lines and push to DB
for line in fo:
    print line.replace('\n','')
    with connection.cursor() as cursor:
        # Create a new record
        sql = "INSERT INTO email_unreg(email,kampan) VALUES (%s, %s)"
        cursor.execute(sql, (line,'Nedorucene'))
        connection.commit()
        print "OK"
print "Finish"