SWS1

HUMAN - 348 amino acids, 414 nm.
http://www.ncbi.nlm.nih.gov/protein/P03999.1
>gi|129203|sp|P03999.1|OPSB_HUMAN RecName: Full=Short-wave-sensitive opsin 1; AltName: Full=Blue cone photoreceptor pigment; AltName: Full=Blue-sensitive opsin; Short=BOP

MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLN
YILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVAGLVTGWSLAFLAFERYIVICKPF
GNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSESYTWFLFIFCFI
VPLSLICFSYTQLLRALKAVAAQQQESATTQKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGL
DLRLVTIPSFFSKSACIYNPIIYCFMNKQFQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN
                                                        |  |  |
        1 mrkmseeefy lfknissvgp wdgpqyhiap vwafylqaaf mgtvfligfp lnamvlvatl 43,46,49
                            |     |      |                       |   |
       61 rykklrqpln yilvnvsfgg fllcifsvfp vfvascngyf vfgrhvcale gflgtvaglv 78,83,90,111,115
      121 tgwslaflaf eryivickpf gnfrfsskha ltvvlatwti gigvsippff gwsrfipegl
      181 qcscgpdwyt vgtkyrsesy twflfifcfi vplslicfsy tqllralkav aaqqqesatt
      241 qkaerevsrm vvvmvgsfcv cyvpyaafam ymvnnrnhgl dlrlvtipsf fsksaciynp
      301 iiycfmnkqf qacimkmvcg kamtdesdtc ssqktevstv sstqvgpn

MOUSE - 346 amino acids, 359 nm.
http://www.ncbi.nlm.nih.gov/protein/P51491.1
>gi|1709475|sp|P51491.1|OPSB_MOUSE RecName: Full=Short-wave-sensitive opsin 1; Short=S opsin; AltName: Full=Blue cone photoreceptor pigment; AltName: Full=Blue-sensitive opsin; Short=BOP; AltName: Full=Short wavelength-sensitive cone opsin

MSGEDDFYLFQNISSVGPWDGPQYHLAPVWAFRLQAAFMGFVFFVGTPLNAIVLVATLHYKKLRQPLNYI
LVNVSLGGFLFCIFSVFTVFIASCHGYFLFGRHVCALEAFLGSVAGLVTGWSLAFLAFERYVVICKPFGS
IRFNSKHALMVVLATWIIGIGVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIIP
LSLICFSYSQLLRTLRAVAAQQQESATTQKAEREVSHMVVVMVGSFCLCYVPYAALAMYMVNNRNHGLDL
RLVTIPAFFSKSSCVYNPIIYCFMNKQFRACILEMVCRKPMADESDVSGSQKTEVSTVSSSKVGPH 38,46,49,50,52,57
                                                  |        |  ||  |    |
        1 msgeddfylf qnissvgpwd gpqyhlapvw afrlqaafmg fvffvgtpln aivlvatlhy
       61 kklrqplnyi lvnvslggfl fcifsvftvf iaschgyflf grhvcaleaf lgsvaglvtg
      121 wslaflafer yvvickpfgs irfnskhalm vvlatwiigi gvsippffgw srfipeglqc
      181 scgpdwytvg tkyrseyytw flfifcfiip lslicfsysq llrtlravaa qqqesattqk
      241 aerevshmvv vmvgsfclcy vpyaalamym vnnrnhgldl rlvtipaffs ksscvynpii
      301 ycfmnkqfra cilemvcrkp madesdvsgs qktevstvss skvgph


ALIGNMENT    |||| ||    |              |      |       V  V| V    |       43,46,49
HUMAN  1    MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATL  60
            M    E++FYLF+NISSVGPWDGPQYH+APVWAF LQAAFMG VF +G PLNA+VLVATL
MOUSE  1    M--SGEDDFYLFQNISSVGPWDGPQYHLAPVWAFRLQAAFMGFVFFVGTPLNAIVLVATL  58

            |                V    V      V  |   |   |         V   V
HUMAN  61   RYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVAGLV  120	78,83,90,111,115
             YKKLRQPLNYILVNVS GGFL CIFSVF VF+ASC+GYF+FGRHVCALE FLG+VAGLV
MOUSE  59   HYKKLRQPLNYILVNVSLGGFLFCIFSVFTVFIASCHGYFLFGRHVCALEAFLGSVAGLV  118

                         |       ||  |     |      |
HUMAN  121  TGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSRFIPEGL  180
            TGWSLAFLAFERY+VICKPFG+ RF+SKHAL VVLATW IGIGVSIPPFFGWSRFIPEGL
MOUSE  119  TGWSLAFLAFERYVVICKPFGSIRFNSKHALMVVLATWIIGIGVSIPPFFGWSRFIPEGL  178

                              |           |         |    | |
HUMAN  181  QCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKAVAAQQQESATT  240
            QCSCGPDWYTVGTKYRSE YTWFLFIFCFI+PLSLICFSY+QLLR L+AVAAQQQESATT
MOUSE  179  QCSCGPDWYTVGTKYRSEYYTWFLFIFCFIIPLSLICFSYSQLLRTLRAVAAQQQESATT  238

                    |          |       |                    |     | |
HUMAN  241  QKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNP  300
            QKAEREVS MVVVMVGSFC+CYVPYAA AMYMVNNRNHGLDLRLVTIP+FFSKS+C+YNP
MOUSE  239  QKAEREVSHMVVVMVGSFCLCYVPYAALAMYMVNNRNHGLDLRLVTIPAFFSKSSCVYNP  298

                      |   ||   | | |    |||           ||   |
HUMAN  301  IIYCFMNKQFQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN  348
            IIYCFMNKQF+ACI++MVC K M DESD   SQKTEVSTVSS++VGP+
MOUSE  299  IIYCFMNKQFRACILEMVCRKPMADESDVSGSQKTEVSTVSSSKVGPH  346

| - a residue that is different
V - one of the 8 residues for spectral tuning

The 8 needed mutations for spectral tuning (numbers for human sequence) (mutations are human > mouse)
T43F
L46F
F49T
F78L
L83F
P90T
G111A
T115S

Human sequence with 8 substituted amino acids from the mouse sequence which tunes the human SWS1 opsin gene to 360 nm.
MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGFVFFIGTPLNAMVLVATL
RYKKLRQPLNYILVNVSLGGFLFCIFSVFTVFVASCNGYFVFGRHVCALEAFLGSVAGLV
TGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSRFIPEGL
QCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKAVAAQQQESATT
QKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNP
IIYCFMNKQFQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN


DNA Sequence - http://www.ncbi.nlm.nih.gov/nuccore/1469901

        1 ttctctccag ttccaagctt gcatcatgaa gatggtgtgt gggaaggcca tgacagatga
       61 atccgacaca tgcagctccc agaaaacaga agtttctact gtctcgtcta cccaagttgg
      121 ccccaactga ggacccaata ttggcctgtt tgcaacagct agaattaaat tttactttta
      181 agtaagtttc tattgtctcc gtcagaaacc aaactactaa aaacacaaaa aagatggtaa
      241 aaggagtgat ggcagtttgg ggagtcaatt tttcattttc ttactattgc cttcttgcct
      301 acaaagctac tgtttccact ggtctatttc agaccaccca aaggccattt caacaatcat
      361 cagtttctac tcct

ttc acc aca g - FFT
t tca cca cag - SPQ
tt cac cac agg - HHR
aag tgg tgt cc - KWC
a agt ggt gtc c - SGV
aa gtg gtg tcc - VVS