def application(environ, start_response):
    status = '200 OK'
    output = 'This is my Website!'

    response_headers = [('Content-type', 'text/plain'),
                        ('Content-Length', str(len(output)))]
    start_response(status, response_headers)

    return [output]

WSGIScriptAlias /wsgi "C:/wsgi_app/wsgi_app.py"
<Directory "C:/wsgi_app">
AllowOverride None
Options indexes FollowSymLinks MultiViews
Require all granted
</Directory>

WSGIScriptAlias /wsgi "C:/wsgi_app/wsgi_app.py"
<Directory "C:/wsgi_app">
AllowOverride None
Options none
Require all granted
</Directory>

[Wed Feb 10 16:17:49.293987 2016] [wsgi:warn] [pid 4448:tid 336] mod_wsgi: Compiled for Python/2.7.9+.
[Wed Feb 10 16:17:49.293987 2016] [wsgi:warn] [pid 4448:tid 336] mod_wsgi: Runtime using Python/2.7.9.
[Wed Feb 10 16:17:49.325187 2016] [mpm_winnt:notice] [pid 4448:tid 336] AH00354: Child: Starting 64 worker threads.
[Wed Feb 10 16:17:52.538793 2016] [wsgi:error] [pid 4448:tid 1040] [client ::1:54402] Target WSGI script not found or unable to stat: C:/wsgi_app/wsgi_app.py