from tkinter import *

class test:
    def __init__(self, root):
        self.variables = []
        for i in range(26):
            self.variables.append(StringVar())

        self.frames = []
        self.labels = []
        self.entrys = []
        for i in range(2):
            self.frames.append(Frame(root))
            for ii in range(26):
                char = str(chr(ord('A') + ii))
                if i == 0:
                    self.labels.append(Label(self.frames[0] , text = char))
                    self.labels[-1].grid(padx=0, pady=0, row=ii, column=i)
                else:
                    self.entrys.append(Entry(self.frames[1], textvariable =self.variables[ii]))
                    self.entrys[-1].grid(padx=0, pady=0, row=ii, column=i)
            self.frames[i].grid(row = 0,column=i)

root = Tk()
root.geometry("200x600+50+50")
T = test(root)
root.mainloop()