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- • Question about Treadmilling (F-Actin, +/-, etc.)
- • How to skip lag phase for F-Actin formation (add preformed F-Actin, or Arp2/3 complex + WASP/NWASP/VCA/ActA + Ligands (if WASP/NWASP) + capping protein + severing protein).
- • Non-oncogenic cycle of HPV
- • How HPV can be integrated without causing oncogenesis (disruption to E6 or E7; or no disruption to E2).
- • 2 experiments to quantify Protein X using antibody (Treatment or FACS/fluorescent cell sorting or centrifugal elusion; followed by ELISA, or Western Blot, or relative quantitative MSMS).
- • Regulation of Protein X
- • ???
- • Diagram/steps for insulin induced glucose uptake.
- • ½ page on machine learning.
- • Diagram/steps for regulation involving allosteric and post-translational modification (glycogen synthesis).
- • IFN-I signalling diagram
- Theory of Practical
- • Plot [Ca2+] vs ATPase activity.
- • Which is the dependent (activity) vs independent variable ([Ca2+]?
- • Why do we use log axis?
- • Describe graph/parameters.
- • Mono- vs Polyclonal Abs
- • Design competition ELISA for IL6.
- • Some people have defective IL6, ELISA still showed presence, why? (Can be non-functional and still have an epitope that folds same way. Ab could be off-target.)
- • Redesign sandwich ELISA for IL6 (2nd anti-IL6-Biotin MAb).
- Exp2 (3972)
- • ???
- • Why do we use primary & secondary Abs, instead of just sticking the enzyme on the primary? (Cost – you don’t have to do a transgenic expression of the Ab-Enzyme fusion, just generate the Ab in the animal you have the secondary Ab for.)
- • Loaded various concentrations of NOS2 to see what should be used for standard, and loaded cell extract. Probed both with polyclonal Ab. Why does my figure look like shit?
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