read1 0 -0.4 -0.5 0.2 0.5 read2 0 -0.4 -0.5 0.2 0.5 0.8 read3 0 -0.4 -0.5 0.2 0.5 read4 0 -0.4 -0.5 0.2 0.5 0.8 read5 0 -0.4 -0.5 0.2 0.5 0.8