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IAPV/dsRNA in Honeybees Experiment

a guest May 20th, 2019 50 Never
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  1. IAPV Experiment:
  2. Part 1
  3. 1) Inject five honeybees with the IAPV virus (write down the time and day you do this) leaving another five alone (control).
  4. 2) Watch the bees for one week, making note of all symptoms and behaviors of the virus in the bees
  5. 3) Make note of the bees' mortality rate with IAPV compared to the date you injected the virus
  6. 4) Compare the experimental bees with the control bees (mortality rate), write down observations and conclusions
  7.  
  8. Part 2
  9. 1) Inject five honeybees with IAPV, leaving the five control bees alone
  10. 2) Feed dsRNA to the five experimental bees on a constant basis every day for the week
  11. 3) Make note of the bees' mortality rate compared to the date you injected the virus
  12. 4) Compare the experimental bees' mortality rate and the control bees' mortality rate, and write down observations and conclusions about the effects that the dsRNA had on the IAPV.
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  16. Notes:
  17. IAPV infection results in more significant changes in gene expression in adult bees than in brood
  18. High genetic diversity exists between different strains of IAPV
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