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Biology AS notes

Apr 11th, 2017
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  1. Biology AS notes
  2.  
  3. 1. Cell Structure
  4. Cell is the basic unit of all living organisms
  5. Tissue is a layer or group of cells of a similar type, which together perform a particular function
  6. Organ is a structure within a multicellular organism that is made up of different types of tissue working together to perform a particular function
  7. System is a group of organs/ distinct structures working together to achieve a particular function
  8. Vascular system a closed system of tubes of spaces conducting fluids. (Xylem and phloem in plants) (Blood vascular system (heart, arteries, veins and capillaries in animals)
  9. Organelle is a functionally and structurally distinct part of a cell
  10. Cytoplasm is the contents (protoplasm) of a cell, excluding the nucleus; consisting of the cytosol and the organelles
  11. Cell Surface Membrane ¬is a very thin membrane (7nm) surrounding all cells; it is partially permeable and controls the exchange of materials between the cell and its environment
  12. Nucleus is a relatively large organelle found in eukaryotic cells. The nucleus contains the cell’s DNA and therefore controls the activities of the cell
  13. Chromatin is the loosely coiled form of chromosome during interphase of the cell cycle; chromatin is made of DNA and proteins and is visible as loosely distributed patches or fibres in the nucleus when stained
  14. Nucleolus is visible as a densely stained body; its function is to manufacture ribosomes using information in its own DNA
  15. Nuclear envelope is two membranes, situated closely together, that surround the nucleus; the envelope is perforated with nuclear pores
  16. Nuclear pores control the exchange of material between the nucleus and nucleoplasm
  17. Nucleoplasm is the contents of the nucleus
  18. Mitochondrion is the organelle where aerobic respiration takes place
  19. Endoplasmic Reticulum (ER) a network of flattened sacs running through the cytoplasm of eukaryotic cells; molecules (particularly proteins) can be transported through the cell inside the sacs. ER is continuous with the outer membrane of the nuclear envelope. Rough Endoplasmic Reticulum (RER) is studded with ribosomes and organises synthesis of proteins and their transport in the cell, whereas Smooth Endoplasmic Reticulum manages the synthesis of lipids and steroids.
  20. Golgi apparatus is an organelle that consists of a stack of flattened sacs, constantly forming at one end and breaking up into Golgi vesicles at the other end; Golgi vesicles carry their contents to various parts of the cell, usually to the cell surface membrane for secretion; the Golgi apparatus collects, sorts and modifies proteins
  21. Ribosome is a tiny organelle found in a large number of cells (70s 20nm in prokaryotes and 80s 25nm in eukaryotes). Ribosomes are made of proteins and ribosomal RNA and consists of two subunits. They are the sites of protein synthesis
  22. Lysosome is a spherical organelle found in eukaryotic cells. It contains hydrolytic enzymes and has a variety of destructive function, such as removal of old cell organelles
  23. Centriole is one of two small, cylindrical structures found just outside the nucleus in animal cells that help control spindle formation during nuclear division
  24. Microtubules are tiny tubes made of proteins alpha and beta tubulin. Alpha and beta tubulin form dimers that are joined end to end to form long protofilaments. 13 such filaments line up alongside each other to form a cylinder with a hollow centre. Has 3 main functions; form cytoskeleton and provide mechanical support, form intracellular transport, and form spindle during cell division
  25. Cell wall is a wall surrounding prokaryote, plant or fungal cells. The wall containing strengthening material that protects the cells from mechanical damage, supports it and prevents it from bursting by osmosis if the cell is surrounded by a solution with higher water potential
  26. Plasmodesmata are pore like structures found in pant cell walls; that form a narrow thread of cytoplasm between neighbouring cells allowing the controlled passage of materials from one cell to another
  27. Vacuole is an organelle found in eukaryotic cells. A large permanent central vacuole is a typical feature of plant cell. Has a variety of functions including; storage of biochemical such as salts, sugars and waste products. Temporary phagocytic vacuoles may also form in animal cells
  28. Tonoplast is the partially permeable membrane that surrounds the plant vacuoles
  29. Chloroplast is the photosynthesis organelle in eukaryotes
  30. Chlorophyll is the green pigment responsible for light capture in algae and plants
  31. Granum is a stack of circular thylakoids in plant
  32. Thylakoids is a flattened, membrane-bound, fluid-filled sac, which is the site of the light-dependant reactions of photosynthesis in chloroplasts
  33. Protoctist a member of the protoctista kingdom
  34. Magnification = (Size of image) / (Actual size)
  35. Prokaryotes
  36. Average diameter: 0.5-5.0 micrometres
  37. DNA: naked, circular, lies freely in cytoplasm
  38. Ribosomes: 70s
  39. Organelles: very few organelles present. No membrane bound organelles present.
  40. Cell wall: Present consisting of either murein or peptidoglycan
  41.  
  42. Eukaryotes
  43. Average diameter: typically 40 micrometres
  44. DNA: contained in nucleus, linear, associated to histone proteins forming chromosomes
  45. Ribosomes: 80s
  46. Organelles: many types of organelles present (extensive compartmentalisation)
  47. Cell wall: Maybe present. (Cellulose cell wall in plants, chitin cell wall in fungi)
  48.  
  49. 2. Biological molecules
  50.  
  51. Macromolecule is a large biological molecule (protein, polysaccharide, nucleic acid, lipids)
  52.  
  53. Polymer is a giant molecule made from several, similar, repeating subunits of monomers (lipids are NOT polymers)
  54.  
  55. Monomer is a relatively simple molecule which is used as the basic building block for the synthesis of a polymer; many monomers are joined together to make a polymer, usually by a condensation reaction (monosaccharides, amino acids, nucleotides)
  56.  
  57. Glycosidic bond A C-O-C link between two monosaccharide molecules, formed by a condensation reaction
  58.  
  59. Amylose is a polymer of alpha glucose monomers linked by 1-4 linkages, forming a linear, curved, compact, helical chain is a constituent of starch
  60.  
  61. Amylopectin is a polymer of alpha glucose monomers linked by both 1-4 and 1-6 linkages, forming a branched chain is a constituent of starch
  62.  
  63. Glycogen is a polysaccharide made of alpha glucose, similar in structure to amylopectin however tend to be even more branched (have more 1-6 linkages). Glycogen granules act as glucose stores in the liver and muscles
  64.  
  65. Cellulose is a polysaccharide made up of beta glucose monomers linked by l-4 linkages. Hydrogen bonds can form between cellulose molecules. 60-70 cellulose molecules can be tightly cross-linked by hydrogen bonds to form micro fibrils are held together by further hydrogen bonding to form cellulose fibres
  66.  
  67. Triglyceride is a lipid whose molecules are made up of a glycerol and three fatty acids
  68.  
  69. Phospholipids is a substance whose molecules are made up of a glycerol, two fatty acids and a phosphate group; a bilayer of phospholipids forms the basic structure of all cell membranes
  70.  
  71. Peptide bond is a C-N link between two amino acid molecules formed by a condensation reaction
  72.  
  73. Polypeptide is a long chain of amino acids formed by condensation reactions between one or more amino acids; proteins are made up of one or more polypeptide chains
  74.  
  75. Primary structure is the sequence of amino acids in a polypeptide chain
  76.  
  77. Secondary structure is the structure of a protein molecule resulting from the regular coiling of folding of the amino acid chain
  78.  
  79. Alpha helix is the helical structure of polypeptide chain held in place by hydrogen bonding
  80.  
  81. Beta pleated sheet is the loose, sheet like structure formed by hydrogen bonding between parallel polypeptide chains
  82.  
  83. Tertiary structure the compact structure of a protein molecule resulting from the three dimensional coiling of the already folded amino acid chain due to; Hydrogen bonds, disulfide bridges, ionic bonds, and weak hydrophobic interactions
  84.  
  85. Quaternary structure the three dimensional arrangement of two or more polypeptides, or of a polypeptide and a prosthetic group (non-protein component)
  86.  
  87. Globular protein is a protein whose molecules are folded into a roughly spherical shape, which is often water soluable and metabolically active, as hydrophobic R groups point inwards maintaining the shape of the molecule, whereas hydrophilic R-groups point outwards and are on the surface of the molecule maintaining soluability (haemoglobin)
  88.  
  89. Haemoglobin the red pigment found in RBC, whose molecule contains four iron atoms within a globular protein made up of four polypeptides (two alpha globin, and two beta globin molecules), and that combines reversibly with oxygen
  90.  
  91. Fibrous protein is a protein molecule whose molecules who have a relatively long, thin structure that is generally insoluable and metabolically inactive and whose function is usually structural (keratin, collagen)
  92.  
  93. Collagen is a fibrous protein consisting of three polypeptide chains each in the shape of a helix. Three helical polypeptides are wound around each other to form a triple helix. Almost every third amino acid is glycine. Triple helix is held together by hydrogen bonds and some covalent bonds. Covalent bonds form between adjacent triple helix amino acids R-groups, forming fibrils. Staggered fibrils lie alongside each other forming fibres.
  94.  
  95. Water the god send
  96. Good solvent of ions and polar molecules
  97. Transport medium (in vascular systems of plants and animals)
  98. High specific heat capacity makes water resistant to temperature changes
  99. High latent heat of vapourisation allowing organisms to use evaporation as cooling mechanism
  100. Water is most dense at 4 degrees Celsius, so ice floats; reducing the tendency of large bodies of water to freeze completely
  101. High surface tension and cohesion (important in flow of water in vascular tissue)
  102. Water as a reagent important during photosynthesis and hydrolysis mechanisms
  103.  
  104. 3. Enzymes
  105.  
  106. Enzyme is a globular protein molecule that acts as a biological catalyst
  107.  
  108. Active site is a cleft/depression on the outer surface of the enzyme to which the specific substrate molecule is allowed to bind
  109.  
  110. Lock and Key Hypothesis Is the idea that the enzyme has a particular shape into which the substrate fits exactly
  111.  
  112. Induced fit mechanism adds the idea that the enzyme, and substrate can change shape slightly as the substrate molecule enters the enzyme in order to ensure a perfect fit caused by interactions between R-groups at the active site and the substrate
  113.  
  114. Activation Energy is the minimum amount of energy required for particles to collide successfully in order for the reaction to proceed
  115.  
  116. Competitive inhibitor is a molecule which has a shape similar to the substrate and complementary to the active site, and so can occupy the active site and prevent the substrate from binding. This reduces the Km of a substrate but Vmax is the same
  117.  
  118. Non-competitive inhibitor is a molecule that can bind to a site on the enzyme other than the active site (allosteric site). The binding results in a distortion of enzyme shape that ripples across to the active site, and prevents substrate from binding. This reduces the Vmax but the Km of substrate remains the same.
  119.  
  120. 4. Cell membranes and transport
  121.  
  122. Diffusion the net movement of molecules form a region of higher concentration to a region of lower concentration down a concentration gradient, as a result of the random movement of particles.
  123.  
  124. Facilitated diffusion is the diffusion of substance through transport proteins in a cell membrane; the proteins provide hydrophilic areas that allow molecules or ions to pass through which would otherwise be less permeable to them
  125.  
  126. Osmosis is the net movement of water molecules form a region of higher water potential to a region of lower water potential, down the water potential gradient, through a partially permeable membrane
  127.  
  128. Water potential is a measure of tendency of eater to move from one place to another
  129.  
  130. Active transport is the movement of molecule or ions through transport proteins across a cell membrane, against their concentration gradient, using energy from hydrolysis of ATP
  131.  
  132. Endocytosis is the bulk movement of substance into a cell, by the infolding of cell surface membrane to form vesicles containing the substance. It is an active process
  133.  
  134. Exocytosis is the bulk movement of substance out of a cell, by the fusion of vesicles containing the substance with the cell surface membrane. It is an active process
  135.  
  136. Phospholipids is the main constituent of the bilayer. Hydrophobic interior acts as barrier to the movement of polar substances across the cell membrane. Length and saturation of fatty acid tails determines the fluidity of the cell surface membrane. Hydrophilic phosphate heads form hydrogen bonds with the water in aqueous environment, stabilising the membrane
  137.  
  138. Cholesterol is a small molecule with a hydrophilic head and hydrophobic tail that regulates the fluidity and mechanical stability of the bilayer
  139.  
  140. Glycolipid is a phospholipid attached to a short carbohydrate chain that can act as an antigen marker or take part in cell adhesion
  141.  
  142. Glycoprotein is a membrane protein attached to a short carbohydrate chain that can act as a receptor for cell signalling or take part in cell recognition
  143.  
  144. Channel proteins transmembrane proteins that allow facilitated diffusion to occur
  145.  
  146. Carrier proteins membrane proteins that allow active transport to occur through conformational changes in shape
  147.  
  148. 5. Nucleic acids and protein synthesis
  149.  
  150. Nucleotide is the basic monomer unit that make up nucleic acid polymers (DNA, RNA). Nucleotides have 3 components; a nitrogenous base, a phosphate group, and a pentose sugar.
  151. DNA molecules are made of two polynucleotide strands lying side by side, running in opposite directions, held by hydrogen bonding between complementary purines and pyrimidines.
  152. (Purine A pairs with pyrimidine T (or U in RNA) with 2 H bonds)
  153. (Purine G always pairs with pyrimidine C with 3 H bonds)
  154.  
  155. DNA (Deoxyribonucleic acid) is a polynucleotide that contains the deoxyribose pentose sugar, and contains four bases; Adenine (A), Thymine (T), Guanine (G), Cytosine (C).
  156. RNA (Ribonucleic acid) is a polynucleotide that contains the ribose pentose sugar, and contains four bases; Adenine (A), Uracil (U), Guanine (G), Cytosine (C)
  157. Polynucleotide is the polymer resulting from the formation of phosphodiester bonds between nucleotides by condensation reaction linking the 5-carbon of one sugar and the 3-carbon of the next, and so the polynucleotide is said to have 3’ and 5’ ends
  158. DNA replication is the process of synthesising two new molecules of DNA from a parent molecule of DNA through semi-conservative replication, in which the new molecule consists of one new strand and one strand from the parent DNA molecule
  159.  
  160. 1. DNA double helix unwinds as the hydrogen bonds between the bases break (catalyse by DNA helicase)
  161. 2. In nucleoplasm there are free activated nucleotides
  162. 3. Bases of activated nucleotides pair up with its complementary base on the old DNA strands
  163. 4. DNA polymerase enzyme seals the sugar phosphate back bone by forming phosphodiester bonds between adjacent nucleotides
  164. 5. Extra phosphates are released from activated nucleotides
  165. 6. Two DNA molecules are formed consisting of one new strand and one parent strand
  166.  
  167. Protein synthesis is the process by which the genetic code is used to determine the sequence of amino acids in the formation of proteins. It is a two step-process comprising of transcription and translation
  168.  
  169. Transcription is the process in which genetic information on DNA is copied into an mRNA molecule
  170.  
  171. 1. DNA double helix unwinds as hydrogen bonds between bases break
  172. 2. RNA polymerase enzyme binds to promoter region
  173. 3. Free RNA molecules’ nitrogenous bases bind to complementary bases on DNA template strand through hydrogen bonding
  174. 4. RNA polymerase seals sugar phosphate backbone by forming phosphodiester bonds between adjacent nucleotides
  175. 5. RNA polymerase reaches terminator sequence and unbinds from DNA
  176. 6. H bonds between nitrogenous bases break, mRNA forms
  177. 7. DNA double helix reformed at end of transcription
  178.  
  179. Translation is the process in which genetic information carried on mRNA is used to synthesize polypeptide molecule
  180.  
  181. 1. mRNA molecules attach to ribosomes in cytoplasm
  182. 2. tRNA molecule in cytoplasm have site for protein attachment on one end, and a specific anticodon on the other end
  183. 3. tRNA carries specific amino acid to mRNA molecule
  184. 4. anticodon of tRNA binds to specific codon on mRNA
  185. 5. second tRNA attaches to mRNA molecule
  186. 6. Peptide bonds formed between amino acids of adjacent tRNA molecules
  187. 7. Peptide bond formation catalysed by enzyme peptidyl transferase
  188.  
  189. 6. Mitotic cell cycle
  190.  
  191. Chromosomes is a thread like structure consisting of DNA tightly coiled around histone proteins that support its structure.
  192.  
  193. Cell cycle is the series of events that take place leading to its division and duplication into two daughter cells consisting of; interphase, nuclear division and cell division
  194.  
  195. 1. During interphase cell carries out its normal function
  196. 2. Sell is signalled in order to replicate
  197. 3. DNA replicates so each chromosome consists of two sister chromatids in S phase
  198. 4. During G1 cells make RNA, enzymes and other proteins needed for growth
  199. 5. During G2 New DNA is checked for errors, and preparations are made for replication
  200. 6. Nucleus divides into two at M phase
  201.  
  202. Mitosis is the process by which the nucleus replicates consisting of 4 stages; Prophase, Metaphase, Anaphase, and Telophase
  203.  
  204.  
  205.  
  206.  
  207.  
  208.  
  209. Prophase
  210.  
  211. 1. Chromosomes condense- chromatids coil up and become shorter and thicker
  212. 2. Nuclear envelope breaks down
  213. 3. Nucleolus breaks down
  214. 4. Centrosomes replicate just before prophase and move to opposite poles of the cell to set up spindle apparatus
  215.  
  216. Metaphase
  217.  
  218. 1. Centrosomes reach poles and form spindle apparatus
  219. 2. Chromosomes line up across equator of spindle, attached to spindle by the centromere
  220.  
  221. Anaphase
  222.  
  223. 1. Spindle fibres constrict (microtubules break down)
  224. 2. Sister chromatids migrate to opposite poles centromere first, pulled by spindle fibres
  225.  
  226. Telophase
  227.  
  228. 1. Nucleolus begins to reform
  229. 2. Nuclear envelope begins to reform
  230. 3. Remains of spindle broken down
  231. 4. Chromatids reach poles of spindle and uncoil
  232.  
  233. Cytokinesis is the division of cytoplasm and cell into two by constriction of the edges of the cell membrane
  234.  
  235. Biological significance of mitosis
  236.  
  237. 1. Growth
  238. 2. Replacement of cells and repair of tissue
  239. 3. Asexual reproduction (vegetative propagation)
  240. 4. Immune response
  241.  
  242. Centromere is the constricted central region holding two sister chromatids together to which microtubules of spindle attach via kinetochore
  243.  
  244. Kinetochores are protein molecules that bind specifically to DNA at centromere and microtubules
  245.  
  246. Centrosome is an organelle that consists of a pair of centrioles surrounded by a large number of proteins that control the production of microtubules that act as MTOCs for spindle construction
  247.  
  248. Telomere is a structure that seals the ends of chromosomes in order to ensure that when DNA is replicated the ends of the molecules are included
  249.  
  250. Telomerase is the enzyme that adds extra DNA to the molecule that acts as the molecules telomere
  251.  
  252. Stem cell is an undifferentiated cell which is capable of giving rise to indefinitely more cells of the same type, and from which certain other cells arise by differentiation
  253.  
  254. Cancer is a disease caused by an uncontrolled division of abnormal cells in the body
  255.  
  256. 1. Carcinogens cause mutations in cell, transforming oncogenes
  257. 2. Cancerous cell does not respond to growth inhibition signals so continues to divide
  258. 3. Cancerous cells rapidly divide mitotically
  259. 4. Cancerous cells are not removed by immune system , and take up nutrients for division
  260. 5. Rapid mitosis takes place
  261. 6. Tumour becomes larger, characteristics of cell become abnormal
  262. 7. Tumour supplied with blood and lymph
  263. 8. Tumour metastasizes, cells invade other tissue and form secondary cancers
  264.  
  265. 7. Transport in plants
  266.  
  267. Transpiration is the loss of water vapour from a plant to its environment, by diffusion down a water potential gradient; most transpiration takes places though the stomata in the leave
  268.  
  269. Factors affecting transpiration:
  270.  
  271. Humidity
  272. Air movement
  273. Light intensity
  274. Temperature
  275.  
  276. Parenchyma is thin walled cells used as packing tissue; forming cortex in roots and stems, modified parenchyma containing chloroplast make up leaf mesophyll.
  277. Have many functions such as;
  278. 1. food storage (starch),
  279. 2. when turgid they help support plant preventing wilting,
  280. 3. air spaces between cells allow gas exchange
  281.  
  282. Collenchyma Is modified parenchyma with extra cellulose deposits ate corners of the cell providing extra strength. Forms midrib of leaves
  283.  
  284. Sclerenchyma fibres are long elongated cells with lignified walls that help support the plant
  285.  
  286. Mesophyll is the internal tissue of a leaf blade consisting of an upper layer of palisade mesophyll (main photosynthetic tissue) and a lower spongy mesophyll with large air spaces for gas exchange
  287. Upper epidermis is a thin transparent layer allowing light to reach mesophyll, covered with thick waxy cuticle to prevent water loss
  288. Lower epidermis contains stomata for gas exchange
  289. Stoma is a pore in the epidermis of a leaf, bounded by two guard cells and is needed for efficient gas exchange
  290. Transport of water from leaf to atmosphere
  291. 1. Water vapour diffuses from an air space through an open stoma. Carried away from leaf surface by air movements, marinating water potential gradient
  292. 2. Water evaporates from mesophyll cell walls into the sub stomatal air space
  293. 3. Water moves through mesophyll cell wall, or from cytoplasm into cell wall
  294. 4. Water leaves a xylem vessel through a pit and enter the cytoplasm or cell wall of mesophyll cell
  295. 5. Water moves up the xylem vessel to replace the water lost from leaf
  296. Xylem consists primarily of xylem vessel elements and is responsible for mechanical support and transport of water and mineral ions.
  297. Vessel elements are elongated, dead cells with an empty lumen, lignified walls, and no end walls between neighbouring vessel elements forming a long hollow continuous tube called the xylem vessel.
  298.  
  299. Xylem vessel elements have pits not laid down with lignin that allow lateral movement of water to neighbouring cells to prevent an air lock, and allow water to reach surrounding living cells
  300.  
  301. The movement of water up through xylem vessels is by mass flow
  302. Water molecules form hydrogen bonds with each other (cohesion)
  303. Water molecules are also attracted to cellulose and lignin (adhesion)
  304. Cohesive and adhesive forces allow water to move through xylem as a continuous column
  305.  
  306. Transport of water from root to stem and leaf
  307.  
  308. 1. Removal of water from xylem vessels in the leaf reduces the hydrostatic pressure
  309. 2. Hydrostatic pressure at base of plant is high
  310. 3. Water moves up xylem vessel in continuous channels due to difference in pressures
  311.  
  312. Root pressure is the increase in hydrostatic pressure at the base of plant, in which cells surrounding the xylem pump solutes into xylem by active transport. Decreasing the water potential in xylem causes more water to enter xylem by osmosis increasing the hydrostatic pressure
  313. Endodermis is a one cell thick layer that surrounds vascular tissue in stems and roots
  314. From root hair to xylem
  315. 1. Water potential in xylem vessels is lower than in cortex cells
  316. 2. Water can move across cortex either through the apoplast or symplast pathways
  317. a. Apoplast pathway is when water moves through cells along cell walls
  318. b. Symplast pathway is when water moves through cell through cytoplasm and the interconnecting plasmodesmata
  319. 3. Once the water reaches the endodermis the apoplast pathway is blocked as cells in the endodermis have a thick waxy band of suberin deposited in their cell walls (Casparian strip)
  320. 4. Water can only move through passage cells that do not have extensive suberin depositing through symplast pathway.
  321. 5. Once across endodermis water moves down concentration gradient through pericycle into xylem
  322. From soil into root hair
  323. 1. Water moves into root hairs by osmosis down a water potential gradient
  324. 2. Cytoplasm of root hairs has large quantities of dissolved inorganic ions and organic substances to maintain the water potential gradient
  325. Translocation is the process of transporting assimilates from the source to the sink by mass flow. A hydrostatic pressure gradient is created by the active loading of sucrose at the source, and the active unloading of sucrose at sink.
  326. Loading sucrose into phloem
  327. 1. Sucrose is loaded into a companion cell or directly into a sieve tube element by active transport
  328. 2. Hydrogen ions are actively pumped out of companion cell into its cell wall adjacent to a source
  329. 3. This creates a large excess of hydrogen ions in the apoplast pathway
  330. 4. Hydrogen ions move back into the companion cell down the concentration gradient through co-transporter protein for hydrogen and sucrose
  331. 5. Sucrose carried into companion cell against its concentration gradient
  332. 6. Sucrose molecules diffuse into sieve tube element through plasmodesmatal links
  333.  
  334. Phloem primarily consists of sieve tube elements with associated companion cells, responsible for translocation of assimilates such as sucrose through a plant
  335. Sieve tube element is a living cell that has cell wall, cell surface membrane, cytoplasm, ER and mitochondria. However; the amount of cytoplasm is very small and only forms a thin layer lining the inside of the cell wall. There is no nucleus or ribosomes. Sieve elements form sieve plates with neighbouring sieve elements
  336. Companion cell has the ‘typical’ structure of a plant cell and is associated closely to a sieve tube element by numerous plasmodesmata
  337. Xerophytes are plant species that have adapted to survive in extreme conditions
  338. Main adaptations of xerophytes
  339. 1. Thick waxy cuticle to reduce water loss
  340. 2. Epidermal hairs to retain moisture around leaf surface
  341. 3. Leaves reduced to spines to reduce surface area (cacti)
  342. 4. Stomata are found in pits
  343. 8. Transport in mammals
  344. Closed double circulatory system Blood always remains within the blood vessels so is closed, and for one completer journey around the body the blood flows through the heart twice for the systemic circulation and the pulmonary circulation.
  345. Artery is a blood vessel that transports blood, swiftly and at high pressures, to tissues
  346.  
  347.  
  348. Arteries and Veins both have walls made up of 3 layers:
  349. 1. An inner endothelium (squamous epithelium) which is smooth to minimise friction with moving blood
  350. 2. A tunica media containing smooth muscle, collagen, and elastic fibres
  351. 3. A tunica externa containing collagen and elastic fibres
  352. Structure and function of Artery
  353. 1. Thick tunica externa capable of withstanding high blood pressure that develop in artery
  354. 2. Thick tunica media has large amounts of elastic fibres, that allow the artery to stretch and recoil while blood is moving at high pressures in order to maintain and even out blood flow
  355. Arterioles forms as arteries branch and help control the volume of blood reaching into tissue
  356. Capillaries allow exchange of substance between blood and tissues. Capillaries are made up of a single layer of endothelial cells and has an approximate diameter of 7 micrometres. Red blood cells squeeze through capillaries and a brought to close proximity with respiring cells
  357. Veins return blood to the heart
  358. Structure and function of Vein
  359. 1. Veins have semi-lunar valves at regular intervals to prevent back flow of blood
  360. 2. Pressure in the vein is produced by skeletal muscles contracting close to it
  361. Red Blood Cells (RBC) cell containing haemoglobin pigment for transport of oxygen around the body
  362. Structure and function of RBC
  363. 1. RBC have a biconcave disc shape providing large surface area to volume ratio
  364. 2. RBC are very small so can squeeze through capillaries
  365. 3. RBC have no nucleus, no mitochondria and no ER to maximise space for haemoglobin
  366. 4. RBC are flexible
  367. Haemoglobin dissociation curve
  368. The S shaped curve is caused by the effect of binding of oxygen to haemoglobin. When an oxygen molecule binds to haemoglobin, the haemoglobin molecule distorts is shape and has a greater affinity for a second oxygen molecule to bind and so on and so forth.
  369. The Bohr shift is the movement of the oxygen dissociation curve to the right at high partial pressures of carbon dioxide. At high partial pressures of carbon dioxide oxyhaemoglobin dissociates more readily and can be explained as so:
  370. 1. Carbon dioxide is continually reproduced by respiring cells
  371. 2. Carbon dioxide diffuses from the cells into the cytoplasm, from where some of which diffuses into RBC
  372. 3. In cytoplasm of RBC carbonic anhydrase enzyme rapidly catalyses reaction between carbon dioxide and oxygen forming carbonic acid
  373. 4. Carbonic acid readily dissociates into hydrogen and bicarbonate ions
  374. 5. Hydrogen ions have a greater affinity from oxygen causing oxyhaemoglobin
  375. 6. Hydrogen combines with haemoglobin forming hameoglobinic acid, and oxygen is released
  376. Acclimatisation is the process in which the body compensates for low partial pressures of oxygen at high altitudes by producing greater number of red blood cells, to increase haemoglobin concentration per unit volume of blood and volume of oxygen absorbed
  377. The heart is the hollow muscular organ that pumps blood through the circulatory system through rhythmic contractions and dilations. The heart is made of cardiac muscle.
  378. Aorta is the largest artery and supplies blood to systemic circulation from heart
  379. Pulmonary artery supplies deoxygenated blood to pulmonary circulation
  380. Venae cava return deoxygenated blood from systemic circulation to heart
  381. Pulmonary vein returns oxygenated blood from pulmonary circulation
  382. Coronary arteries are on the surface of the heart providing oxygenated blood to cardiac tissue
  383. Septum acts as a wall separating the left side of the wall from the right side
  384. The upper chamber in the heart are the atria and the lower chambers are the ventricles
  385. The left atrium and left ventricle are separated by the Bicuspid valve (atrioventricular valve)
  386. The right atrium and right ventricle are separated by the Tricuspid valve (atrioventricular valve)
  387. Cardiac cycle is the sequence of events that occur as the heart beats
  388. Control of the heart beat
  389. 1. Each cardiac cycle begins at the right atrium at the sinoatrial node (SAN) which is myogenic
  390. 2. As the muscle in the SAN contracts it produces an electrical excitation wave which sweeps through all of the muscles in the atria of the heart. This excitation wave causes the atria to contract (atrial systole)
  391. 3. The excitation wave reaches another patch of cells at the base of the right atrium called the atrioventricular node (AVN)
  392. 4. The AVN delays the impulse for a fraction of a second before it travels down into the ventricles (this ensures ventricles contract after atria)
  393. 5. The excitation wave moves swiftly down through the septum, along purkyne tissue
  394. 6. Once the excitation reaches the base of the septum it sweeps upwards, through the ventricle walls causing them to contract (ventricular systole)
  395. 9. Gas exchange and smoking
  396. Functions carried out by the human gas exchange system
  397. 1. Clean and warm the air that enter during breathing
  398. 2. Maximise the surface are for diffusion of oxygen and carbon dioxide between the blood and atmosphere
  399. 3. Minimise the distance for this diffusion
  400. 4. Maintain adequate gradients for this diffusion
  401.  
  402.  
  403. The lungs are in the thoracic cavity surrounded by pleural membrane that enclose an airtight space. The pleura act to lubricate the lungs to provide friction free movement as the lungs are ventilated by the movement of the diaphragm and ribs
  404. Leading from the throat to the lungs is the trachea. At the base of the trachea are two bronchi, which subdivide extensively forming bronchioles. Bronchioles divide to form even narrower respiratory bronchioles which supply alveoli with air. Cartilage in the trachea and bronchi prevent airways from collapsing due to the changes in pressure caused by breathing.
  405. Trachea: C-shaped cartilage, goblet cells, smooth muscle, cilia
  406. Bronchi: ring cartilage, goblet cells, smooth muscle, cilia
  407. Bronchiole: smooth muscle, cilia
  408. Alveoli: site of gas exchange
  409.  
  410. Warming and cleaning the air
  411. 1. As air enters, it is warmed to body temperature, and moistened by evaporation to protect delicate surfaces from desiccation
  412. 2. Particles larger than 5-10 micrometres are trapped in nasal hairs and trapped in mucus lining nasal passage
  413. 3. In trachea and bronchi, mucus is produced by goblet cells of the ciliated epithelium. Each goblet cell is swollen with mucin droplets which have been secreted by the cell
  414. 4. Respiratory macrophages are present to destroy any antigenic particles in lining
  415. Alveoli Structure and function
  416. 1. Alveolar walls contain elastic fibres which stretch and recoil during breathing
  417. 2. Alveoli have one cell thick squamous epithelial wall. Pressed closely against alveoli wall are a network of capillaries with one cell thick endothelial wall (Gas diffuses quickly through short distances)
  418. 3. Breathing constantly replaces expired air with inspired air. Blood in capillaries is constantly being replaced with deoxygenated blood from heart, and oxygenated blood is carried away. (this maintains the concentration gradient for the gases to diffuse)
  419. Tobacco smoke
  420. Main components: Tar (contains carcinogens), carbon monoxide, and nicotine
  421. Chronic Obstructive Pulmonary Diseases (COPD)
  422. Chronic Bronchitis
  423. 1. Tar in cigarette smoke stimulates goblet cells and mucus glands to enlarge and secrete more mucus
  424. 2. Tar inhibits the cleaning action of ciliated epithelium by destroying cilia
  425. 3. Mucus accumulates in bronchioles
  426. 4. Bacteria, viruses, and dirt begin to accumulate in mucus
  427. 5. Damaged epithelia are replaced with scar tissue
  428. 6. Smooth muscles surrounding becomes thicker narrowing air passage
  429.  
  430.  
  431. Emphysema
  432. 1. Phagocytes release enzyme that destroys elastin in alveoli in order to reach infected site in lungs
  433. 2. Elastin is responsible for the recoil of the alveoli during expiration
  434. 3. In absence of elastin, alveoli cannot efficiently stretch and recoil
  435. 4. Bronchioles collapse during expiration, trapping air inside alveoli, which often burst
  436. 5. Large spaces appear in absence of alveoli, reducing surface area of gas exchange system
  437. Lung cancer
  438. 1. Tar in tobacco smoke is carcinogenic
  439. 2. Cause mutations in epithelial cells, which leads to tumour formation (see mitotic cell cycle)
  440. 3. As the cancer develops, it spreads through the bronchial epithelium and enters lymphatic tissue
  441. 4. Cells may break away and form secondary growths (metastasises)
  442. Nicotine
  443. 1. Absorbed readily into blood
  444. 2. Stimulates the nervous system to reduce the diameter of arterioles and release the hormone adrenaline from adrenal glands
  445. 3. Heart rate and blood pressure increase, with reduced blood supply to extremities
  446. 4. Increases damage to arteriole lining resulting in plaque formation (atherosclerosis)
  447. 5. Increases the risk of blood clotting (thrombosis)
  448. Carbon monoxide
  449. 1. Diffuses into RBC and binds permanently to haemoglobin form carboxyhaemoglobin
  450. 2. Reduces the amount of oxygen transported by blood
  451. 10. Infectious disease
  452. Diseases is a physiological or psychological, illness or disorder that leads to poor health; associated to a particular set of signs and symptoms and may or may not be communicable
  453. Cholera:
  454. 1. Pathogen: Vibrio cholerae
  455. 2. Method of transmission: food-borne, water-borne
  456. 3. Site of action of pathogen: wall of small intestine (secrets choleragen toxin, that disrupts function of epithelial lining of intestines causing salts and water to leave the blood)
  457. 4. Clinical features: Severe diarrhoea, loss of water and salts, dehydration, weakness
  458. Transmission of cholera
  459. 1. People do not have access to proper sanitation and uncontaminated food
  460. 2. Infected people who are symptomless carriers contaminate water supply, or handle food or cooking utensils without washing their hands
  461. 3. Eating poorly prepared contaminated seafood
  462.  
  463.  
  464. Treating cholera
  465. 1. Salts and glucose are given intravenously
  466. 2. If people can drink, the they are given oral rehydration therapy
  467. Preventing cholera
  468. 1. Development of sewage and water treatment systems
  469. 2. Do not use human sewage in irrigation of vegetables
  470. Malaria:
  471. 1. Pathogen: Plasmodium falciparum, P.vivax,, P.oval, P.malariae
  472. 2. Method of transmission: insect vector (female anopheles mosquito)
  473. 3. Site of action of pathogen: liver, red blood cells, brain
  474. 4. Clinical features: fever, anaemia, nausea, headaches, muscle pains, shivering, sweating, enlarged spleen
  475. Transmission of malaria
  476. 1. Female anopheles mosquito feed on human blood
  477. 2. If person is infected with plasmodium, mosquito will take up gametes in blood meal
  478. 3. Male and female gametes fuse in the mosquitos gut and develop to infective stages which move to mosquitos salivary glands
  479. 4. The infective stages pass from the mosquito to an uninfected person through mosquito saliva
  480. 5. Plasmodium can also cross across the placenta from mother to foetus
  481. Treating malaria
  482. 1. Anti-malarial drugs used for treatment and as prophylactic drugs
  483. 2. Drugs prescribed must be changed if strain becomes resistant to currently prescribed drug
  484. Preventing malaria
  485. 1. Reduce the number of mosquitos (Biological control measures)
  486. a. Stocking permanent bodies of water (ponds, irrigation, ditches) with fish that feed on mosquito larvae
  487. b. Spraying a preparation containing bacillus thuringinesis, which kills mosquito larvae but is not toxic to other forms of life
  488. 2. Avoid being bitten by mosquitos
  489. a. Sleep beneath mosquito nets
  490. b. Soaking mosquito nets in insecticide
  491. 3. Use drugs to prevent parasite from infecting people
  492. Worldwide control of malaria
  493. Was not successful for two reason:
  494. 1. Plasmodium became resistant to the drugs used to control it
  495. 2. Mosquitos became resistant to DDT and other insecticides that were used at the time
  496.  
  497.  
  498. Worldwide concern over malaria because:
  499. 1. An increase in drug resistant forms of plasmodium
  500. 2. An increase in the proportion of cases caused by P.falciparum (severe malaria)
  501. 3. Difficulty in developing vaccine against malaria
  502. 4. Increasing number of epidemics due to climate change
  503. Factors that may lead to improvement of control of malaria:
  504. 1. Use of modern techniques in gene sequencing and drug design
  505. 2. Development of vaccines targeting different stages of pathogens life cycle
  506. 3. Renewed international will to remove burden of disease in poor parts of the world
  507. Acquired Immune deficiency syndrome (AIDS)
  508. 1. Pathogen: human immunodeficiency virus
  509. 2. Method of transmission: in semen and vaginal fluids during sexual intercourse, infected blood or blood products, contaminated hypodermic syringes, mother to foetus across placenta, at birth, mother to infant in breast milk
  510. 3. Site of action of pathogen: T helper lymphocytes, macrophages, brain cells
  511. 4. Clinical features:
  512. a. HIV infection- flu-like symptoms and then symptomless
  513. b. AIDS- opportunistic infections including; pneumonia, TB, and cancers; weight loss, diarrhoea, fever, sweating, dementia
  514.  
  515. Treating HIV/AIDS
  516.  
  517. 1. No as of yet known cure
  518. 2. Drug therapy can slow down onset
  519. 3. Drugs are expensive and have a variety of side-effects
  520. 4. Drugs are similar to DNA nucleotides and act in order to stop viral replication
  521.  
  522. Preventing HIV/AIDS
  523.  
  524. 1. People are educated about infection to stop spread
  525. 2. Use of condoms, femidoms, and dental dams during sexual intercourse
  526. 3. Contact tracing to identify others who might be HIV+ and control disease spread
  527. 4. Needle exchange schemes to stop spread of disease among drug users
  528. 5. Blood collected from blood donors is heat treated to destroy any virus present
  529. 6. Woman are encouraged not to breast feed to prevent transmission from mother to infant if she is HIV+
  530.  
  531.  
  532.  
  533.  
  534.  
  535.  
  536.  
  537.  
  538.  
  539. Tuberculosis (TB)
  540.  
  541. 1. Pathogen: Mycobacterium tuberculosis, m.bovis
  542. 2. Method of transmission: airborne droplets (m.tuberculosis), via unpasteurised milk and undercooked meat (m.bovis)
  543. 3. Site of action of pathogen: primary infection in lungs; secondary infection in lymph bode, bones and gut
  544. 4. Clinical features: racking cough, coughing blood, chest pain, shortness of breath. Fever, sweating, weight loss
  545.  
  546. Transmission of TB
  547. Increase of incidence in TB worldwide due to:
  548. 1. Drug resistant strains of TB
  549. 2. HIV/AIDS pandemic
  550. 3. Poor housing in inner cities and homelessness
  551. 4. Breakdown of TB control programmes
  552.  
  553. Treating TB
  554.  
  555. If TB is confirmed:
  556. 1. Patient is isolated at most infective stage (particularly if drug resistant strain)
  557. 2. Treatment involves using several drugs to ensure all the bacteria is killed
  558. 3. People who do not complete the course of antibiotics harbour drug resistant bacteria which they can then spread to others
  559.  
  560. Preventing TB
  561.  
  562. 1. Contact tracing
  563. 2. BCG vaccine use to prevent children from getting disease
  564. 3. Cattle are routinely tested for TB and any found to be infected are destroyed
  565.  
  566. Measles
  567.  
  568. 1. Pathogen: Morbillivirus
  569. 2. Method of transmission: droplet infection (highly contagious)
  570. 3. Site of action of pathogen: upper respiratory tract
  571. 4. Clinical features: rash, runny nose, cough, conjunctivitis
  572. 5. Treatment: bed rest and taking medicine to lower the fever
  573.  
  574. Difficulty of eradicating measles
  575.  
  576. 1. One-dose-vaccination programme has not been effective due to poor response. Children require several boosters to develop full immunity
  577. 2. Requires a very high percentage coverage to achieve herd immunity >90%
  578. 3. Vaccine is not thermostable
  579.  
  580.  
  581. Antibiotics are a type of antimicrobial drug that used to destroy microorganisms or inhibit their growth by interfering with some aspect of cell growth or cell metabolism
  582.  
  583. Penicillin
  584.  
  585. 1. Penicillin prevents the formation of cross-links between peptidoglycan polymers in the cell walls of bacteria by inhibiting enzymes that build cross links
  586. 2. Bacterial cell secretes autolysins which make holes in the cell wall
  587. 3. Holes allow walls to stretch so new polypeptide chains can link together
  588. 4. Penicillin prevents cross links from forming
  589. 5. Cell wall becomes progressively weaker
  590. 6. Water enters cell by osmosis causing cell to burst
  591.  
  592. Antibiotic resistance is when a strain of bacteria is no longer sensitive to an antibiotic, either achieved through horizontal transmission (resistance passed to daughter cells during binary fission) or vertical transmission (passed from one species of bacteria through another through conjugation) through plasmids containing genes providing resistance
  593.  
  594. Immunity
  595.  
  596. An antigen is a substance that is foreign to the body and stimulates and immune response
  597.  
  598. An antibody is a glycoprotein (immunoglobulin) made by plasma cells derived from B-lymphocytes, secreted in response to an antigen; the variable region of the antibody molecule is complimentary in shape to its specific antigen
  599.  
  600. The immune response is the complex series of responses of the body to the entry of a foreign antigen; it involves the activity of lymphocytes and phagocytes
  601.  
  602. Non-self refers to any substance or cell that is recognised by the immune system as being foreign and will stimulate an immune response
  603.  
  604. Self refers to substances produced by the body that the immune system does not recognise as foreign; so immune system is not stimulated
  605.  
  606. Phagocytes are white blood cells that protect the body by ingesting foreign, potentially harmful particles (phagocytosis)
  607.  
  608. Neutrophils are a kind of phagocyte that travel through the walls of capillaries and ‘patrol tissue’
  609. During an infection neutrophils are released in large numbers from their stores, but they-are short lived cells
  610.  
  611. Macrophages are a kind of phagocyte that are larger than neutrophils and tend to be found in organs (lungs, liver, lymph, kidney, spleen). After they are made in the bone marrow, they travel in the blood as monocytes, which mature into macrophages once they settle in the organs.
  612. Are long lived cells and play a crucial role in initiating an immune response by becoming antigen presenting cells (APCs)
  613.  
  614. Phagocytosis
  615.  
  616. 1. If pathogens invade the body and cause an infection, some of the cells release histamine
  617. 2. Histamines and antigens released by pathogens attract neutrophils to site of infection
  618. 3. Neutrophils move towards the pathogen which may be clustered together and covered in antibodies
  619. 4. The antibodies further stimulate neutrophils to attack the pathogens
  620. 5. When neutrophil attach to pathogen, the cell surface membrane engulfs the pathogen and traps it within a phagocytic vacuole (endocytosis)
  621. 6. Digestive enzymes are secreted into the vacuole and the pathogen is destroyed
  622.  
  623. Lymphocytes
  624.  
  625. B-lymphocyte remains in the bone marrow until they are mature and then spread throughout the body, concentrating in the lymph nodes and spleen
  626.  
  627. Primary immune response
  628. 1. When an antigen enters the body for the first time (antigen presentation)
  629. 2. Small number of B-lymphocytes in blood with antibody receptor complimentary to antigen bind to antigen and take part in immune response (clonal selection)
  630. 3. Selected B cells divide by mitosis. Some of the daughter cells divide into plasma cells, and others develop into memory cells
  631. 4. Plasma cells secrete antibodies against target antigen to be neutralised
  632. Secondary immune response
  633. 5. The antigen re-enters the body
  634. 6. Memory cells produced in primary response, respond to produce a larger number of plasma cells and memory cells
  635. 7. The response is much faster and a greater concentration of antibodies is produced, thereby suppressing the infection
  636.  
  637. Immunological memory- refers to the ability of the immune system to respond more rapidly and effectively to a pathogen that has been encountered previously
  638.  
  639. Antibody is a globular glycoproteins consisting of four polypeptide chains (two heavy chains and two light chains). Disulphide bonds hold the chains together. Each molecule has two identical antigen binding sites which are formed by both light and heavy chains. The antigen binding sites form the variable region which is different on each type of antibody molecule. The hinge region gives the flexibility for the antibody molecule to bind around the antigen
  640.  
  641. Mechanisms of antibody action:
  642. 1. Combine with antigen preventing it from entering cell
  643. 2. Attach to flagella of bacteria reducing their activity
  644. 3. Agglutination of bacteria preventing spread
  645. 4. Punch holes in bacterial cell walls
  646. 5. Coat bacteria increasing ease of phagocytosis
  647. 6. Combine and neutralise antigens
  648.  
  649.  
  650. T-lymphocytes leave the bone marrow, and collect in the thymus where they mature. T-lymphocytes have t-cell receptors that are specific to an antigen, and are activated if the antigen is encountered on one of the host cells.
  651.  
  652. 1. Antigen enters the body
  653. 2. Macrophage engulfs antigen and displays antigen on its cell surface membrane becoming an antigen presenting cell (APC)
  654. 3. T-lymphocytes with T-receptor complimentary to the antigen, bind to the antigen on an APC and are activated
  655. Helper T cells
  656. 4. Helper T cells divide by mitosis to form helper T cells and memory cells
  657. 5. T helper cells secrete cytokines that;
  658. a. Stimulate appropriate B cells to divide, develop into plasma cells and secret antibodies
  659. b. Stimulate macrophages to carry out phagocytosis more vigorously
  660. Killer T cells
  661. 4. Killer T cells divides by mitosis to form killer T cells and memory cells
  662. 5. T cells recognise the antigen, attach themselves to the surface of infected cells, and secret toxic substances (hydrogen peroxide), killing the body cell and the pathogen inside it
  663.  
  664. Active immunity is immunity gained wen an antigen enters the body, an immune response occurs and antibodies are produced by plasma cells
  665.  
  666. Passive immunity is immunity gained without an immune response; antibodies against pathogen are introduced into the body instead
  667.  
  668. Natural immunity is immunity gained by being infected or by receiving from the mother across the placenta or through breast milk
  669.  
  670. Passive immunity is immunity gained either by vaccination or by injecting antibodies
  671.  
  672. Vaccination is giving a vaccine containing antigens for a disease, either by injection or by mouth; vaccination confers artificial active immunity
  673.  
  674. Problems with Vaccines
  675.  
  676. 1. Poor response as a result of weak immune system, or malnutrition
  677. 2. Live virus may be passed out in faces during primary response and capable of spreading (solution is Herd immunity, where a large number of people are vaccinated at the same time, interrupting transmission in a population so that those who haven’t received vaccine are also protected
  678. 3. Antigenic variation occurs when a pathogenic strain mutates regularly (virus) resulting in antigenic drift (which could lead to antigenic shift), as a result vaccine is no longer effective. Pathogen cannot be targeted as it does not have a stable antigen to develop vaccine against
  679. 4. Antigenic concealment is when pathogens evade attack by the immune system by living inside host cells/ using host cells to evade detection
  680.  
  681.  
  682. How the eradication programme of smallpox succeeded
  683.  
  684. 1. Variola virus was stable; it did not mutate and change its surface antigens, so same vaccine can be used throughout campaign so therefore it was cheap to produce in bulk
  685. 2. The vaccine was made from a harmless strain of a similar virus so it was more effective as virus was ‘live’
  686. 3. The vaccine was freeze-dried, and thermostable so could be kept it high temperatures , so could be used in the tropics
  687. 4. Infected people were easy to identify
  688. 5. Vaccine was easy to administer and was even more effective after the development of the stainless-steel reusable needle
  689. 6. Smallpox did not linger in the body after infection (no reservoir for infection)
  690. 7. Virus did not affect animals, made it easier to break the transmission cycle
  691. 8. Teenagers were enthusiastic vaccinators and educators, being especially effective in remote areas
  692.  
  693. Autoimmune disease is a condition in which the body produces antibodies against self-antigens, leading to the deterioration and sometimes destruction of tissue
  694.  
  695. Myasthenia Gravis is an autoimmune condition resulting in progressive muscle weakness. Antibodies are secreted that bind to acetylcholine receptors on the cell surface membrane of muscle cells blocking the transmission from the motor neurone
  696.  
  697. Monoclonal antibodies is the large scale production of a particular antibody using hybridoma cells.
  698.  
  699. 1. Antigen injected into mouse spleen to stimulate immune response in mouse resulting in plasma cells that produce antibodies against specific antigen
  700. 2. Plasma cells from mouse spleen are collected
  701. 3. Plasma cells are fused with myeloma cells producing hybridoma cells
  702. 4. Hybridoma cells are tested to identify plasma cells that produces specific antibody
  703. 5. Hybridoma cell is isolated and cultured
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