Protein-B2-calc-ML-2019

sample	run 1	run 2	run 3	Average	blanked	ug/ml	ug/L	FORMULA
Culture T= 0	1.745	1.562	1.462	1.590	1.173	2780.333333	2780333.333	y=0.0004x+0.0612
filtrate T= 0	1.207	1.096	1.213	1.172	0.756	1736.166667	1736166.667	x=(y-0.0612)/0.0004
Culture at stationary	1.642	1.558	1.558	1.586	1.170	2772	2772000
culture filtrate (from us)	1.337	1.316	1.319	1.324	0.908	2117	2117000
Culture Supernatant (at stationary)	1.5	1.598	1.453	1.517	1.101	2599.5	2599500
Lyate 	-	-	-			-
Lysate supernatant (applied to column)	0.659	0.791	0.64	0.697	0.281	548.6666667	548666.6667
tube 1	0.5	0.495	0.491	0.495	0.079	45.33333333	45333.33333
tube 2	0.667	0.699	0.757	0.708	0.292	576.1666667	576166.6667
tube 3 (free dried with PBS dilution)	0.551	0.523	0.593	0.556	0.140	196.1666667	196166.6667

std (ug/ml)	blanked
125	0.051
250	0.085
500	0.309
750	0.498
1000	0.544
1500	0.760
2000	0.823