AIM 1: To characterize the macrophage polarization potential of Resveratrol, Shikonin,
Thymoquinone, Chlorogenic acid, Neem leaf extract : Azadirachtin
RAW 264.7 (murine macrophages)
Cell culture : check for cellular proliferation and viability
Stimulation with LPS/IFN—y (M1) and IL-4 (M2), Treatment with difl'erent
concentrations of test compounds
Check for presence of phenotype specific markers :
PCR, western blotting
Optimisation of compound concentration
2 experimental sets :
1. RAW 264-7 + LPS/IL-4 + test compounds
2- RAW 264-7 + test compounds

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