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- AIM 1: To characterize the macrophage polarization potential of Resveratrol, Shikonin,
- Thymoquinone, Chlorogenic acid, Neem leaf extract : Azadirachtin
- RAW 264.7 (murine macrophages)
- Cell culture : check for cellular proliferation and viability
- Stimulation with LPS/IFN—y (M1) and IL-4 (M2), Treatment with difl'erent
- concentrations of test compounds
- Check for presence of phenotype specific markers :
- PCR, western blotting
- Optimisation of compound concentration
- 2 experimental sets :
- 1. RAW 264-7 + LPS/IL-4 + test compounds
- 2- RAW 264-7 + test compounds
- Free Online OCR newocr.com
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