datafile = "C:\Users\589016\Desktop\Test.csv"
countTable = read.delim(datafile, sep = ";", header = F)
countTable
     V1  V2  V3   V4
b1  1,2   4  40   30
b2  3,2 3,4  50  3,1
b3    4 4,3  20 30,2
b4    5   3 3,2  3,4
b5  3,2 4,5   4  4,1
b6    0   5 2,1    3
b7  4,5   0 3,4  2,1
b8    3   0 0,1  2,1
b9    2 3,2 0,1    0
b10 0,4   0 0,1    1

ExpDesign = data.frame(row.names = colnames(countTable), condition = c("control", "control", "case", "case"))
ExpDesign
   condition
V1   control
V2   control
V3      case
V4      case
cds = DESeqDataSetFromMatrix(countData = countTable, colData=ExpDesign$condition, design=~ExpDesign$condition)

Error in `rownames<-`(`*tmp*`, value = c("V1", "V2", "V3", "V4")) :
  attempt to set 'rownames' on an object with no dimensions