use lib '/home/egstation/applications/bioperl-live'; #we check out the latest SVN code use Bio::TreeIO; use Bio::Tree::Tree; use Data::Dumper; use Bio::Tree::Draw::Cladogram; my $treeio = Bio::TreeIO->new(-file => "HSF.phyloxml", -format => "phyloxml"); my $tree = $treeio->next_tree; my @nodes = $tree->get_nodes; my $nodeid; for (my $i=0; $i<=$#nodes;$i++){ if($nodes[$i]->id eq 'LOC_Os08g43334.2'){ $nodeid=$i; } } my $ance = $nodes[$nodeid]->ancestor; my $count=0; for my $child($ance->get_all_Descendents){ if($child->id){ $count++; } } my $copy = $ance; while($count<5){ $count=0; my $new_anc = $copy->ancestor; for my $child($new_anc->get_all_Descendents){ if($child->id){ $count++; } } $copy = $new_anc; } my @children; for my $child($copy->get_all_Descendents){ push(@children,$child); } my $new_root = $tree->get_lca( -nodes => \@children); my $subtree = Bio::Tree::Tree->new(-root => $new_root, -nodelete => 1); my $image_output = "test.eps"; my $obj1 = Bio::Tree::Draw::Cladogram->new(-tree => $subtree, -top => 10, -bottom => 10,); $obj1->print(-file => $image_output);