Views on a 10000000-length Rle subject
views:
start end width
[1] 1 1000 1000 [100 100 100 100 100 100 100 100 100 100 ...]
[2] 1001 2000 1000 [190 190 190 190 190 190 190 190 190 190 ...]
[3] 2001 3000 1000 [280 280 280 280 280 280 280 280 280 280 ...]
[4] 3001 4000 1000 [370 370 370 370 370 370 370 370 370 370 ...]
[5] 4001 5000 1000 [460 460 460 460 460 460 460 460 460 460 ...]
... ... ... ... ...
[9996] 995001 9996000 9001000 [89650 89650 89650 89650 89650 89650 ...]
[9997] 996001 9997000 9001000 [89740 89740 89740 89740 89740 89740 ...]
[9998] 997001 9998000 9001000 [89830 89830 89830 89830 89830 89830 ...]
[9999] 998001 9999000 9001000 [89920 89920 89920 89920 89920 89920 ...]
[10000] 999001 10000000 9001000 [90010 90010 90010 90010 90010 90010 ...]
[1] 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
library('GenomicRanges')
# generating data frame
df=data.frame(chrom=rep('Chr1',100000),start=seq(1,1000000,by=1000),end=seq(1000,10000000,by=1000),strand=rep("+",100000))
# making GRanges object
gr=GRanges(seqnames=as.vector(df[,1]),IRanges(start=df[,2],end=df[,3]),strand=df[,4])
# obtaining coverage using function coverage in the form of RLE object
gr.cov=coverage(gr)
# generating views for specific start and end
gr.views=Views(gr.cov[[1]],start=seq(1,1000000,by=1000),end=seq(1000,10000000,by=1000))
# putting in temp variable
d=gr.views
# this following code calculates the matrix (where each line is 20 points) for 10 lines
# reduce or increase the number in the outermost sapply loop to increase/decrease the lines to be calculated
sapply(1:10,function(j)
sapply(1:20,
function(i)as.numeric(
format(
mean(
as(d[[j]][(
seq(0,length(d[[j]]),floor(length(d[[j]])/20))+1)[i]:
c((seq(0,length(d[[j]]),floor(length(d[[j]])/20)))[
-length((seq(0,length(d[[j]]),floor(length(d[[j]])/20))))
],length(d[[j]]))[i+1]],
"RangedData")$score),
digits=2)
)
)
)
genes <- GRanges(seqnames, IRanges(geneStarts, geneEnds))
n <- 50L
starts0 <- Map(function(...) floor(seq(...)), start(genes), end(genes),
MoreArgs=list(length.out=n + 1L))
ends <- lapply(starts0, function(x) floor(x)[-1])
starts <- lapply(starts0, function(x) x[-length(x)])
v <- Views(gr.cov[[1]], start=unlist(starts), end=unlist(ends))
split(viewMeans(v), rep(seq_along(genes), each=n))
> (x <- Rle(c(rep(100, 10), rep(200, 10))))
numeric-Rle of length 20 with 2 runs
Lengths: 10 10
Values : 100 200
> runValue(x)
[1] 100 200
> runLength(x)
[1] 10 10